Gene sequencing chip and gene sequencing device

Abstract

The invention provides a gene sequencing chip. According to the gene sequencing chip, grooves are formed in a cover, and 4 channels or 2 channels are formed between the grooves and a silicon chip, sothat the unit flux is high; the silicon chip is provided with a plurality of reaction chambers, each reaction chamber can capture and fix a DNA molecule, so that the DNA molecule cannot move along with fluid, and high-flux optical sequencing is realized; the 4-channel chip not only can guarantee data flux and save cost, but also can separately load different test piece samples, and can meet the applications of complete genome sequencing, complete exon sequencing, transcriptome sequencing and the like; the test piece consumption and the reagent consumption of the 2-channel chip are reduced by half, so that the 2-channel chip can be used for quick detection of small and short test pieces; and besides, own positioning holes and a clamping hole protecting frame are arranged on the periphery ofthe chip, so that the chip can be accurately positioned, a clamping hole can also be stretched to enable the protecting frame to be in micro deformation, and the chip is convenient to load; and the assembling production efficiency is high, and the gene sequencing chip is suitable for batch large-scale production.

Description

technical field [0001] The invention relates to the technical field of gene sequencing, in particular to a gene sequencing chip and a gene sequencing device. Background technique [0002] With the development of gene sequencing technology, the second-generation sequencing technology, which performs continuous cycles of chemical reactions such as fluorescent labeling, fluorescent imaging, deblocking, and removal of fluorophores, has been widely used in whole genome sequencing. Transcriptome sequencing, metagenomic sequencing, etc., the corresponding sequencing platforms and products are constantly changing. As an important device in the process of sequencing the base sequence of specific DNA fragments, the gene sequencing chip is moving towards high-throughput and low-cost driven by sequencing technology. Rapid development. [0003] Traditional sequencing chips are usually equipped with a large overall liquid flow channel, which requires a large amount of DNA molecule sample...

AI Technical Summary

Problems solved by technology

[0003]Traditional sequencing chips are usually equipped with a large overall liquid flow channel, which requires a large amount of DNA molecule samples and chemical reaction reagents, which makes the cost of sequencing reagents high ; Moreover, the unit throughput of this type of chip is low, and at the same time, the time cost is high, which is not conducive to rapid measurement and limits the application; in terms of preparation methods, the traditional sequencing chip is directly bonded by glass and silicon chips through glue, and it is easy to dislocate or The scrap rate of impurities such as air bubbles is high; there are also glues replaced with sealants, but because there are many types of sealants, and they may react with various reagents at the same time, the selection is difficult, and none of them can meet the requirements of large-scale batch production; and ordinary Adhesive glue or sealant solidified and shaped thicker, together with the depth of the flow channel becomes larger, increasing the consumption of various reagents; in addition, the prior art has recorded an integrated structure chip, which processes the flow channel or 3D on the surface of the silicon wafer. Printing or injection molding pressurized cover plate, the molding process of the internal flow channel structure of the chip is complex and difficult, and at the same time, due to the high price of silicon, the material loss and high cost of opening the flow channel are large, so the above-mentioned sequencing chips are difficult to meet High-throughput and low-cost requirements for next-generation sequencing chips

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