A kind of method for obtaining potato leaf roll virus genus Brassica yellow virus by using peach aphid

Abstract

The invention relates to a method for obtaining Brassica yellows virus (BrYV) of the genus Brassica yellows virus (BrYV) by using Myzus persicae. The specific steps are as follows: S1: obtaining a virus transgenic plant carrying Brassica yellows virus ; S2: obtaining non-toxic aphids for feeding poison; S3: using the non-toxic green peach aphids to obtain viruses from transgenic plants containing virus genomes and frozen diseased leaves; S4: detecting the virus transmission ability of the green peach aphids after obtaining the virus. Potato leaf curl virus such as brassica yellowing virus and other virus acquisition methods established by the present invention can well overcome the difficulty of long-term preservation, space occupation, inconvenient transportation and easy toxin source of plants existing in peach aphid by feeding diseased plants. The technical bottlenecks such as mixing have broadened the simplicity, speed and reliability of vector insect poisoning and species resistance identification.

Description

technical field [0001] The invention belongs to the field of agricultural science and technology. A kind of potato leafroll virus (Brassicayellows virus, Brassicayellows virus, BrYV) method. Background technique [0002] Brassica yellow virus (BrYV) is a newly discovered virus of the genus Polerovirus (Polerovirus), and some scholars also call it a strain of turnip yellows virus (Turnip yellows virus). Distributed in provinces and cities, BrYV mainly infects cabbage (Brassica oleracea var. ) and other cruciferous crops, causing crop yellowing and leaf curling symptoms in the field (Xiang et al., 2011). Wang et al. reported that the virus can also infect field Solanaceae plant tobacco (Nicotianatabacum L.) (Wang et al., 2015). There are three main genotypes of the virus: BrYV-A, BrYV-B and BrYV-C (Xiang et al., 2011; Zhang et al., 2014), the identity is about 93%. Zhang et al. (Zhang et al., 2015) respectively constructed full-length invasive cDNA clones of these three ge...

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Problems solved by technology

However, there is no report on the use of aphids to obtain infectious potato leaf curl virus from frozen diseased leaves, and there is no formal report on the successful application of transgenic plants with the full-length genome of the virus as a virus source.

BrYV can only be transmitted by aphids under natural conditions. Although there are virus-infectious cDNA clones mediated by Agrobacterium, the inoculation efficiency is unstable and cannot completely replace the natural mediator inoculation conditions. Therefore, it is necessary to solve the problem of virus source preservation and can The method of aphid transmission

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