Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method of a photoelectrochemical biosensor for simultaneous detection of dual myocardial markers

A myocardial marker and biosensor technology, which can be used in electrochemical variables of materials, scientific instruments, and material analysis by optical means, etc., can solve problems such as limiting wide application, and achieve the effects of accurate results, high sensitivity and simple operation.

Active Publication Date: 2021-11-30
XINYANG NORMAL UNIVERSITY
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, it has been reported that a photoelectrochemical sensing platform was constructed based on the different photoelectric signals generated by two different enzyme-labeled signal probes in two detection solutions to simultaneously detect two cardiac markers, but this method requires the use of two Different enzymes and detection base solutions containing specific enzymes greatly limit its wide application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of a photoelectrochemical biosensor for simultaneous detection of dual myocardial markers
  • Preparation method of a photoelectrochemical biosensor for simultaneous detection of dual myocardial markers
  • Preparation method of a photoelectrochemical biosensor for simultaneous detection of dual myocardial markers

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0027] The preparation method of the photoelectrochemical biosensor for simultaneously detecting double myocardial markers of the present invention comprises the following steps:

[0028] 1) Deposit nano gold on the surface of ITO electrode to obtain Au / ITO electrode;

[0029] In order to obtain Au / ITO electrodes with better conductivity, under the deposition voltage from 0.7V-1.2V, gold nanoparticles were deposited by cyclic voltammetry on the surface of clean ITO electrodes, and washed with phosphate buffer solution of pH=7.4 Electrode surface, get Au / ITO electrode, the result is as follows figure 1 as shown, figure 1 A- figure 1 F are the SEM images of electrodeposited gold nanoparticles at deposition voltages of 0.7V, 0.8V, 0.9V, 1.0V, 1.1V, and 1.2V, respectively. The inset is a CV (current-voltage curve) graph, in which cyclic voltammetry The parameters are set as follows: the initial potential is -0.2V, the scan rate is 0.1V / S, figure 1 The SEM image in shows the ef...

Embodiment 1

[0039] The preparation method of the photoelectrochemical biosensor for simultaneous detection of double myocardial markers in this embodiment comprises the following steps:

[0040] 1) The deposition voltage is 0.9V, gold nanoparticles are deposited on the surface of a clean ITO electrode, and the electrode surface is washed with a phosphate buffer solution with pH=7.4 to obtain an Au / ITO electrode.

[0041] 2) Dissolve 1.0 mg of CdS nanowires in 1.0 mL of 0.1 mg / mL chitosan solution, the concentration of CdS is 1.0 mg / mL, and 1.0 mg of SnNb 2 o 6 Nanosheets were dissolved in 1.0 mL of 0.1 mg / mL chitosan solution, SnNb 2 o 6 The concentration is 1.0mg / mL, take 20 microliters of the mixed solution of CdS and chitosan and 20 microliters of SnNb 2 o 6 The mixed solution of chitosan and chitosan is added dropwise in two different regions of the Au / ITO electrode obtained in step 1), and dried at 60°C to obtain a region containing both CdS / Au / ITO and SnNb 2 o 6 / Au / ITO modifi...

Embodiment 2

[0044] The preparation method of the photoelectrochemical biosensor for simultaneous detection of double myocardial markers in this embodiment comprises the following steps:

[0045] 1) The deposition voltage is 0.9V, gold nanoparticles are deposited on the surface of a clean ITO electrode, and the electrode surface is washed with a phosphate buffer solution with pH=7.4 to obtain an Au / ITO electrode.

[0046] 2) Dissolve 0.5 mg of CdS nanowires in 1.0 mL of 0.11 mg / mL chitosan solution, the concentration of CdS is 0.5 mg / mL, and 0.5 mg of SnNb 2 o 6 Nanosheets were dissolved in 1.0 mL of 0.11 mg / mL chitosan solution, SnNb 2 o 6 The concentration is 0.5mg / mL, take 20 microliters of the mixed solution of CdS and chitosan and 20 microliters of SnNb 2 o 6 The mixed solution of chitosan and chitosan is added dropwise in two different regions of the Au / ITO electrode obtained in step 1), and dried at 65°C to obtain a region containing both CdS / Au / ITO and SnNb 2 o 6 / Au / ITO modi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention relates to a preparation method of a photoelectrochemical biosensor for simultaneous detection of double myocardial markers, comprising the following steps: 1) depositing nano gold on the surface of an ITO electrode to obtain an Au / ITO electrode; 2) obtaining an Au / ITO electrode in step 1) Two different regions of the electrode are decorated with CdS and SnNb respectively 2 o 6 , resulting in regions containing CdS / Au / ITO and SnNb 2 o 6 / Au / ITO region modified electrode; 3) CdS / Au / ITO region and SnNb modified electrode prepared in step 2) 2 o 6 The / Au / ITO area was modified with glutaraldehyde, the Myo antibody was added dropwise on the CdS / Au / ITO area, and the SnNb 2 o 6 The cTn I antibody was added dropwise on the / Au / ITO region to obtain a photoelectrochemical biosensor for simultaneous detection of dual myocardial markers. The photoelectrochemical biosensor for simultaneous detection of dual myocardial markers prepared by the invention has the advantages of simple operation, accurate results, and the like.

Description

technical field [0001] The invention relates to the technical field of photoelectrochemical analysis, in particular to a method for preparing a photoelectrochemical biosensor for simultaneous detection of double myocardial markers. Background technique [0002] Photoelectrochemical analysis is an analysis method developed on the basis of electrochemistry. It combines photochemical analysis with electrochemical analysis. Due to its advantages such as simple operation, low background signal, easy miniaturization, and high sensitivity, photoelectrochemical analysis is widely used. Protein analysis, cell analysis, nucleic acid analysis, and drug analysis. [0003] Cardiovascular disease has attracted widespread attention due to its high morbidity and mortality. How to detect cardiovascular disease quickly and accurately is very important. In recent years, many cardiac markers including cardiac troponin T, myoglobin (Myo) and cardiac troponin I (cTnI) have been reported for the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/17G01N27/327G01N27/48
CPCG01N21/17G01N27/3278G01N27/48
Inventor 曹俊涛刘洋马燕刘彦明
Owner XINYANG NORMAL UNIVERSITY