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Reducibility protein polypeptide and preparation method and application thereof

A protein polypeptide and protein technology, applied in the field of preparation of reduced protein polypeptide, can solve the problems of no biological activity, unfavorable absorption and utilization, unfavorable cell absorption and utilization, etc., and achieve the effect of small particle size and favorable absorption.

Pending Publication Date: 2019-11-08
GUANGZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The elemental selenium prepared by the reduction of inorganic selenium is often easy to aggregate, forming gray or black insoluble aggregated elemental selenium, which has no biological activity and is not conducive to cell absorption and utilization
The latest research shows that nano-selenium particles can be prepared by using macromolecules such as protein or chitosan as a dispersant or embedding agent for elemental selenium, and can prevent the aggregation of elemental selenium, but the particle size of the formed macromolecular nano-selenium particles is relatively small. Big, not conducive to the body's absorption and utilization, nutrition and functionality are relatively simple

Method used

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  • Reducibility protein polypeptide and preparation method and application thereof
  • Reducibility protein polypeptide and preparation method and application thereof
  • Reducibility protein polypeptide and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Preparation of protein and polypeptide: collect tuna meat, wash it to make minced meat, and mix it with water according to the solid-liquid mass ratio of 1:5. Adjust the pH to 7, add 2.0% protease, and enzymolyze at 50°C for 30 minutes; place in a water bath at 90°C, inactivate for 20 minutes, centrifuge after cooling, and take the supernatant to obtain the reduced protein polypeptide.

[0033] Preparation of active nano-selenium: Take 80 mL of the reducing protein polypeptide with a concentration of 5.0 mg / mL, mix it with 40 mL of 0.005 M sodium selenite or sodium selenate with a pH of 6.5, and react at 60 ° C for 30 minutes to obtain the active A solution of nano-selenium.

Embodiment 2

[0035] Preparation of protein and polypeptide: collect tuna meat, wash it to make minced meat, and mix it with water according to the solid-liquid ratio of 1:20. Adjust the pH to 9.5, add 10% protease, and hydrolyze at 25°C for 500 minutes; place in a water bath at 90°C, inactivate for 20 minutes, centrifuge after cooling, and take the supernatant to obtain the reduced protein polypeptide.

[0036] Preparation of active nano-selenium: Take 100 mL of the reducing protein polypeptide with a concentration of 500 mg / mL, mix it with 0.1 mL of 10M sodium selenite or sodium selenate with a pH of 7.0, and react at 4°C for 600 minutes to obtain The solution.

Embodiment 3

[0038] Preparation of protein polypeptide: Weigh the egg white liquid and mix it with water according to the solid-liquid ratio of 1:10. Adjust the pH to 6, add 3% protease, and enzymolyze at 37°C for 350 minutes; place in a water bath at 90°C, inactivate for 20 minutes, centrifuge after cooling, and take the supernatant to obtain the reduced protein polypeptide.

[0039] Preparation of active nano-selenium: Take 100 mL of the reducing protein polypeptide with a concentration of 100 mg / mL, mix it with 10 mL of 0.1M sodium selenite or sodium selenate with a pH of 7.0, and react at 50°C for 200 minutes to obtain the active nano-selenium Selenium solution.

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Abstract

The invention discloses a reducibility protein polypeptide and a preparation method and application thereof. The reducibility protein polypeptide is prepared from enzymatic hydrolysis of egg white proteins and / or tuna meat proteins and / or peanut meal proteins with cysteine proteases and / or ALcalase alkaline proteases and / or neutral proteins, and has reducibility. Through the interaction of the reducibility protein polypeptide with sodium selenite or sodium selenate, active nano selenium with the biological function can be prepared, and two-component absorption and dual-function effects of a body on the active nano selenium can be realized. According to the reducibility protein polypeptide, the particle size of the prepared nano selenium is small, and the absorption and utilization of the nano selenium by the body are facilitated; and the reducibility protein polypeptide also has antioxidant activity, is expected to become a novel, safe and efficient selenium supplement with outstandingfunctions, and is widely used in the fields of animal production, feed production and application or human health food.

Description

technical field [0001] The invention belongs to the field of biochemical industry or biopharmaceuticals, and relates to a reduced protein polypeptide, in particular to a preparation method and application of the reduced protein polypeptide. Background technique [0002] Selenium is one of the essential trace elements for humans and animals. Selenium has an important relationship with the body's antioxidant capacity, anti-cancer effects, anti-virus, and immune functions. A large number of studies and clinical trials have shown that selenium deficiency can cause dysfunction in some important organs in the human body, resulting in various diseases, such as tumors, cardiovascular diseases, and Keshan disease. At present, the main selenium supplement is sodium selenite (Na 2 SeO 3 ) or sodium selenate (Na 2 SeO 4 ) and other inorganic salts, the body's absorption and residual toxicity of inorganic salts are not easy to control, and there is a risk of selenium poisoning. [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C01B19/02A23L33/16A23K20/20
CPCC12P21/06C01B19/02A23L33/16A23K20/30A23V2002/00A23V2200/30A23V2250/1626
Inventor 曾庆祝姜文怡唐红艳
Owner GUANGZHOU UNIVERSITY
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