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A kind of dna probe and making method

A technology of DNA probe and production method, which is applied in the direction of material excitation analysis, fluorescence/phosphorescence, instruments, etc., to achieve the effect of small structure, high sensitivity and simple production process

Active Publication Date: 2022-03-18
SHENZHEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using molecular beacons as probe DNA, the process of preparing optical fiber SERS gene probes is relatively simple, and there is currently no such DNA probe and production method

Method used

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  • A kind of dna probe and making method
  • A kind of dna probe and making method
  • A kind of dna probe and making method

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Embodiment 1

[0031] The optical fiber 1 is one of ordinary refractive index gradient type, refractive index graded single-mode fiber, multimode fiber, and silica fiber with a core size of 10 microns to 600 microns, preferably a silica fiber with a core size of 200 μm. For the optical fiber, the metal nanoparticle substrate 2 includes but not limited to spherical, triangular, plate-shaped, and cubic metal particles. In this embodiment, the metal nanoparticle material is silver. The probe sensitive layer 3 includes, A single-stranded DNA molecule 301 with complementary ends, one end is modified with a sulfhydryl (-S) or sulfhydryl (-SH) functional group 302, and one end is modified with a fluorescent molecule 303, the fluorescent molecule 303 can be FAM, Cy5, ROX, etc., This kind of DNA molecule coils into a hairpin structure in a free state.

[0032] The above-mentioned tapered optical fiber Raman DNA probe can be made by the following steps:

[0033] Such as image 3 As shown in , the op...

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Abstract

The present invention is applicable to the field of optical fiber sensing, and provides a tapered optical fiber Raman DNA probe structure and a manufacturing method thereof. The probe structure includes a pyramid-shaped optical fiber substrate 1 with a roughened surface, and a layer of metal nanoparticle-reinforced substrate. 2 and a DNA probe sensitive layer 3 of a hairpin structure, the present invention also provides a method for making this tapered optical fiber Raman DNA probe, comprising the following steps: first utilize femtosecond laser micromachining technology to make surface roughening Pyramid-shaped optical fiber 1; using electrostatic adsorption or light-induced growth methods to modify and grow metal nanoparticles 2 on the surface of the tapered optical fiber; finally, the highly sensitive optical fiber is realized by assembling the hairpin structure DNA probe sensitive layer 3 The DNA probe, the tapered optical fiber Raman DNA probe provided by the present invention has a simple structure and high sensitivity, and can realize highly sensitive and specific detection of DNA target fragments.

Description

technical field [0001] The invention belongs to the field of optical fiber sensing, in particular to a tapered optical fiber Raman DNA probe and a manufacturing method. Background technique [0002] As the carrier of genetic information, genes can control the traits of individual organisms by guiding protein synthesis, and pass genetic information to offspring through replication. Therefore, gene analysis and detection have important application value in the fields of medical diagnosis and eugenics. For diseases that seriously affect human health, more than half of the symptoms are related to genetic genes; therefore, it is of great significance to realize the clinical detection of disease-causing genes. So far, there are many methods for genetic detection, such as high-throughput sequencing and melting curve analysis , denaturing liquid chromatography analysis, etc., but these methods are cumbersome or difficult to achieve clinical detection. In contrast, fiber optic DNA pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N21/65
CPCG01N21/645G01N21/6428G01N21/658
Inventor 耿优福许译文王丽娜易多洪学明李学金
Owner SHENZHEN UNIV
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