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Method for determining concentration of licorice glycoside C2 in blood plasma

A technology of liquiritin and blood plasma, which is applied in the field of measuring the concentration of liquiritin C2 in plasma, and achieves the effects of high sensitivity, simple method and simple operation

Inactive Publication Date: 2019-11-15
JIANGSU PROVINCIAL HOSPITAL OF TCM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is no literature report on the in vivo assay method of liquiritin C2

Method used

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  • Method for determining concentration of licorice glycoside C2 in blood plasma
  • Method for determining concentration of licorice glycoside C2 in blood plasma
  • Method for determining concentration of licorice glycoside C2 in blood plasma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0031] Determination of Liquiritin C2 Concentration in Rat Plasma

[0032] 1. Experimental materials and instruments

[0033] Liquiritin C2 (MUST-19080103) and naringenin (MUST-19032406) were purchased from Chengdu Mansite Biotechnology Co., Ltd. Test water: ultrapure water; Methanol: chromatographically pure (Merck Company).

[0034] API 4000LC-MS / MS triple quadrupole mass spectrometer, equipped with electrospray ionization source (ESI), chromatography workstation: Analyst 1.6; Agilent 1200 high performance liquid chromatography; AE240 electronic balance (Shanghai Mettler-Toledo Co., Ltd. company); MICRO-17R refrigerated centrifuge (Thermo, USA); WH-2 micro-vortex mixer (Shanghai Huxi Analytical Instrument Factory); Drict-Q5 ultrapure water machine (Millipore, France).

[0035] 2. Liquid conditions

[0036] 1. Liquid chromatography conditions

[0037] Chromatographic column: Waters XTerra MS C18 column (dp 3.5μm, 50mm ID×2.1mm); mobile phase: acetonitrile-2mmol L -1 Ammon...

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Abstract

The invention discloses a method for determining concentration of licorice glycoside C2 in blood plasma. A liquid chromatography-mass spectrometry system is adopted for determination, firstly, a to-be-determined sample is taken, a certain quantity of organic solvent protein precipitation solutions are added, a chromatographic column is used for separation after pretreatment, and a mass spectrometry detector is used for detection. The method is rapid, accurate, high in sensitivity, simple and convenient to operate and suitable for determining the concentration of licorice glycoside C2 in the blood plasma.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a method for measuring the concentration of liquiritin C2 in blood plasma. Background technique [0002] Licorice glycoside C2 is a new flavonoid compound isolated from the dry root and rhizome of Glycyrrhiza uralensis Fisch., a perennial herb of the genus Licorice. In vitro experiments have proved that liquiritin C2 has significant anti-ulcer, anti-AIDS, anti-inflammatory and other biological activities, and is a new drug source compound with good development prospects. Its structural formula is as follows: [0003] [0004] There is no literature report on the in vivo assay method of liquiritin C2. The invention establishes a method for measuring liquiritin C2 in rats, and conducts exploratory research on drug metabolism of liquiritin C2 through the method. Contents of the invention [0005] Purpose of the invention: Aiming at the problems exist...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 戴国梁张农山刘史佳居文政高骁君于茜吴磊
Owner JIANGSU PROVINCIAL HOSPITAL OF TCM
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