A kind of kit and method for simultaneous determination of ft3 and ft4 in blood

A technology of FT4 and reagent kits, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of low detection accuracy, narrow dynamic range, and large sample volume, and achieve low quantitative lower limit, wide dynamic range, and simple operation Effect

Active Publication Date: 2022-07-12
HEFEI NOVA-MS BIOTECH MEDICAL EQUIP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the large sample volume required for the detection of FT3 and FT4 in the prior art, the sample needs to be derivatized, the detection takes a long time, the detection accuracy is not high, and the operation is complicated, the specificity is low, the difference between batches is large, the sensitivity is low, and the dynamic range is low. Narrow technical problem, the application provides a kit for simultaneous determination of FT3 and FT4 in blood, including ultrafiltration device, buffer, FT3 and FT4 mixed calibrator, FT3 and FT4 mixed quality control, FT3 and FT4 mixed stable isotope Internal standard solution, this protocol is easy to operate, has high specificity, small batch-to-batch difference, high sensitivity, and wide dynamic range, which can meet the clinical requirements for FT3 and FT4 detection

Method used

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  • A kind of kit and method for simultaneous determination of ft3 and ft4 in blood
  • A kind of kit and method for simultaneous determination of ft3 and ft4 in blood
  • A kind of kit and method for simultaneous determination of ft3 and ft4 in blood

Examples

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Effect test

Embodiment 1

[0039] A kit for simultaneous determination of FT3 and FT4 in blood of this embodiment, the kit includes the following components:

[0040] Table 1 Components of the kit for simultaneous determination of FT3 and FT4 in blood

[0041]

[0042]

[0043] In this embodiment, the molecular weight cut-off of the ultrafiltration device of component 1 is 50K.

[0044] The preparation of component 2 buffer solution includes: 12.570g 4-hydroxyethylpiperazineethanesulfonic acid and 0.900g sodium hydroxide, dissolved in 1L purified water, shaken, and ultrasonically mixed; take 15mL of the above buffer and put it into a clean brown glass bottle, closed with parafilm.

[0045] Components 3 to 8 are mixed calibrators of FT3 and FT4 with different concentrations, which are used to draw calibration curves. Among them, the concentrations of FT3 calibrators are 40, 20, 10, 4, 2, and 1 pg / mL, and the concentrations of FT4 calibrators are 160, 80, 40, 16, 8, 4 pg / mL.

[0046] Components 9...

Embodiment 2

[0051] A kit for simultaneous determination of FT3 and FT4 in blood of this example is basically the same as Example 1, the difference is that sodium chloride, potassium dihydrogen phosphate, magnesium sulfate, urea, calcium chloride are added to component 2 .

[0052] The specific preparation includes: 12.570g 4-hydroxyethylpiperazineethanesulfonic acid, 5.265g sodium chloride, 0.224g potassium dihydrogen phosphate, 0.275g magnesium sulfate heptahydrate, 0.300g urea, 0.275g calcium chloride dihydrate, dissolved In 1L of purified water, shake and sonicate to mix evenly; take 15mL of the above buffer into a clean brown glass bottle, and seal it with parafilm.

[0053] In this example, additional components are added to enhance the pH buffering capacity and ionic strength of the buffer, so that the parallelism of the ultrafiltration effect of different clinical serum samples is increased by 5-10% compared with Example 1, that is, different clinical serum samples are used in this...

Embodiment 3

[0055] A kit for simultaneous determination of FT3 and FT4 in blood in this embodiment is basically the same as that in Embodiment 2, except that the molecular weight cut-off of the ultrafiltration device is 3K.

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Abstract

The invention belongs to the technical field of hormone detection, and relates to a kit and method for simultaneously measuring FT3 and FT4 in blood. In view of the large sample volume required for the detection of FT3 and FT4 in the prior art, the sample needs to be derivatized, the detection takes a long time, the detection accuracy is not high, and the operation is complicated, the specificity is low, the batch-to-batch variation is large, the sensitivity is low, and the dynamic range is low. Narrow technical problem, this application provides a kit for simultaneous determination of FT3 and FT4 in blood, including ultrafiltration device, buffer, FT3 and FT4 mixed calibrator, FT3 and FT4 mixed quality control, FT3 and FT4 mixed stable isotope As an internal standard solution, this protocol is easy to operate, has high specificity, small batch-to-batch variation, high sensitivity, and wide dynamic range, which can meet the clinical detection of FT3 and FT4. The present application also provides a method for simultaneous determination of FT3 and FT4 in blood without derivatization treatment, which simplifies the detection steps.

Description

technical field [0001] The invention belongs to the technical field of hormone detection, and in particular, relates to a kit and method for simultaneously measuring FT3 and FT4 in blood. Background technique [0002] The thyroid gland is an endocrine gland that secretes thyroid hormones, which have extensive and strong physiological effects on the body. Triiodothyronine (T3) and thyroxine (T4) are two main thyroid hormones, which can promote individual growth and development, increase the body's basal metabolic rate, improve the oxidative decomposition of sugar, fat and protein, increase the In addition, thyroid hormone also has important physiological effects on the central nervous system, cardiovascular system, and digestive system. About 10% of T3 and T4 in blood come from the thyroid gland, and the remaining about 90% of T3 is produced by T4 after deiodination metabolism in peripheral tissues. In the blood circulation, T3 and T4 exist in two forms: bound and free, mos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8822
Inventor 彭军江振作陈文慧刘超谢沙莎
Owner HEFEI NOVA-MS BIOTECH MEDICAL EQUIP CO LTD
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