Preparation method of test contrast card for rapidly determining residual content of organophosphorus pesticides

A technology of organophosphorus and test cards, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions, can solve the problem of being unable to determine the approximate content of pesticide residues, and meet the professional quality requirements The effect of low cost, good effect and simple preparation method

Inactive Publication Date: 2019-11-19
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The method of the present invention solves the problem that the conventional pesticide residue detection card cannot determine the approximate content of pesticide residues; by the method of the present invention and utilizing the existing pesticide residue detection card, a group of color test control cards for different organic phosphorus can be produced, from the comparison The color of the card can determine the concentration range of the sample to be tested. The method of the present invention is universal; the process of the present invention is simple and easy to operate; it is suitable for industrial production after being made into a finished product; it can be qualitative and quantitative

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: the preparation method of methimphos test control card, concrete operations are as follows:

[0020] (1) Take the powder of the standard phenimin, add acetonitrile to dissolve and make a 0.104mg / mL solution;

[0021] (2) Preparation of diluent: Take 13.61g of potassium dihydrogen phosphate, add distilled water to dissolve it, and distill it to 500mL to obtain a 0.2mol / L potassium dihydrogen phosphate solution, take 4g of sodium hydroxide, add distilled water to dissolve it And dilute to 500mL with distilled water to get 0.2moL / L sodium hydroxide solution, mix 50mL of 0.2moL / L potassium dihydrogen phosphate solution with 35ml of 0.2moL / L sodium hydroxide solution, dilute with freshly boiled cold water to 200mL, shake well to get a buffer solution with pH 7.2 as a diluent;

[0022] (3) Take the solution in step (1) and dilute it with the diluent in step (2) to a concentration of 0.104×10 -2 mg / mL, 0.208×10 -3 mg / mL, 0.104×10 -3 mg / mL, 0.521×10 -4 mg / mL ...

Embodiment 2

[0025] Embodiment 2: the preparation method of parathion test contrast card, concrete operations are as follows:

[0026] (1) Take parathion standard powder, add acetonitrile to dissolve and make a 0.128mg / mL solution;

[0027] (2) Preparation of diluent: Take 2g of sodium hydroxide, add distilled water to dissolve it, and dilute to 500mL with distilled water to obtain a 0.1mol / L sodium hydroxide solution, take 1.36g of potassium dihydrogen phosphate, add 0.1mol / L hydrogen Sodium oxide 79mL, dilute to 200mL with distilled water to obtain a pH7.4 buffer solution, as a diluent;

[0028] (3) Take the solution in step (1) and dilute it with the diluent in step (2) to a concentration of 0.128×10 -2 mg / mL, 0.256×10 -3 mg / mL, 0.128×10 -3 mg / mL, 0.640×10 -4 mg / mL parathion solution, while the diluent is used as a blank control solution;

[0029] (4) Pipette 50 μL of parathion solution and blank control solution of different concentrations in step (3) and add them dropwise on the ...

Embodiment 3

[0031] Embodiment 3: the preparation method of methyl parathion test contrast card, concrete operation is as follows:

[0032] (1) Take methyl parathion standard powder, add acetonitrile to dissolve and make a 0.120 mg / mL solution;

[0033] (2) Preparation of diluent: Take 5.59g of dipotassium hydrogen phosphate and 0.41g of potassium dihydrogen phosphate, add distilled water to dissolve them, and dilute to 1000mL to obtain a buffer solution with a pH of 8.0 as a diluent;

[0034] (3) Take the solution in step (1) and dilute it with the diluent in step (2) to a concentration of 0.120×10 -2 mg / mL, 0.240×10 -3 mg / mL, 0.150×10 -3 mg / mL, 0.8×10 -4 mg / mL methyl parathion solution, while the diluent is used as a blank control solution;

[0035](4) Pipette 50 μL of different concentrations of methyl parathion solution and blank control solution in step (3) and add them dropwise on the pesticide residue test card. After standing at room temperature for 15 minutes, pinch with your ...

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PUM

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Abstract

The invention discloses a preparation method of a test contrast card for rapidly determining the residual content of organophosphorus pesticides. According to the method, a phosphate buffer solution with a pH value ranging from 7.2 to 8.0 is used as a diluent to dilute an organic phosphorus standard substance dissolved by acetonitrile, so that organic phosphorus solutions with different concentrations ranging from 0.2*10<-4> to 0.2*10<-2>mg/mL can be obtained; the organic phosphorus solutions with different concentrations are added dropwise onto pesticide residue test cards respectively, and at the same time, a diluent is dropwise added to a pesticide residue test card, so that the pesticide residue test card can serve as a blank contrast card; and after the pesticide residue test cards show colors, the plurality of pesticide residue test cards which show colors in the concentration range of 0.2*10<-4> to 0.2*10<-2> mg/mL are test contrast cards. With the test contrast card prepared bythe preparation method of the invention adopted, the general range of organic phosphorus pesticide residue content in a to-be-detected sample can be rapidly determined. The preparation method of thecontrast card is simple, is suitable for outdoor and laboratory rapid contrast, and has a bright application prospect.

Description

technical field [0001] The invention belongs to the technical field of organophosphorus pesticide residue detection, in particular to a method for preparing a test control card for quickly determining the approximate range of organophosphorus pesticide residue content in a substance to be tested. Background technique [0002] Along with economic development, people have higher and higher requirements on material level, and the problem of pesticide residues in food has gradually attracted more and more attention. Organophosphorus pesticides usually refer to organic compounds such as phosphorous (phosphonic) esters or thiophosphonic esters. They have been around for decades and have always played an important role in pesticides because of their high efficiency, rapidity, and broad spectrum. , its use accounts for about 70% of all pesticides, but it also causes some pollution to the environment and seriously affects food safety while promoting agricultural development. Organoph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 胡旭佳陈丽丽
Owner KUNMING UNIV OF SCI & TECH
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