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Construction method and application of synovial sarcoma heterogenous transplanting mouse model

A synovial sarcoma and mouse model technology, applied in the field of establishment of tumor transplantation mouse models, can solve the problems of high failure rate of clinical trials of anti-tumor drugs, lack of immune system, etc., to prevent medical social burden, easy tumor metastasis, The effect of increasing effectiveness

Active Publication Date: 2019-11-22
THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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Problems solved by technology

The study found that the cells continuously adapt to the in vitro environment during the passage process, and the biological characteristics are not consistent with the tumor cells in the body. Defects lead to a high failure rate in clinical trials of anticancer drugs

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  • Construction method and application of synovial sarcoma heterogenous transplanting mouse model
  • Construction method and application of synovial sarcoma heterogenous transplanting mouse model
  • Construction method and application of synovial sarcoma heterogenous transplanting mouse model

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Embodiment Construction

[0029]After being reviewed and approved by the Hospital Ethics Committee, all patients signed an informed consent form before sample collection. The collected tissues underwent histopathological examination. The tumor tissue blocks of patients with synovial sarcoma confirmed by pathology were stored in sterile normal saline and placed on ice. superior. The tissue blocks were divided into 3 parts, the first part was directly processed under sterile conditions (see below for details) and xenografted into immunodeficient mice; the second part was inserted into 10% DMSO (dimethyl sulfoxide), 90% FBS (fetal bovine serum) and 1% NEAA (non-essential amino acids) in DMEM (a medium containing various amino acids and glucose), gradient frozen to -80 degrees, and stored in liquid nitrogen; Part 3 put Quick-frozen in liquid nitrogen, and then stored in liquid nitrogen. Among them, the first part is used for modeling, the second part is used for resuscitation and vaccination, and the thir...

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Abstract

The invention provides a construction method and application of a synovial sarcoma heterogenous transplanting mouse model. The method comprises the steps of firstly, collecting a synovial sarcoma patient tumor tissue block, and performing heterogenous in-situ transplanting of the fresh patient tumor tissue block into an immune deficiency mouse body under the sterile condition, wherein heterogenousin-situ planting includes the steps of selecting a 2-6-week old immune deficiency mouse, the mouse is consistent with the patient in gender, the collected fresh patient tumor tissue block is soaked with a sterile incubation solution containing fetal calf serum, the mouse is fixed after anesthesia, the patient tumor tissue block is planted into the joint capsule of the mouse, an incision is sewn,and the mouse is bred so that a tumor in the joint capsule can grow. Compared with subcutaneous modeling, the in-situ modeling condition is more real, the screened medicine scheme can be better used for the patient, and on the basis of the in-situ modeling theory, tumor metastasis is more likely to happen compared with subcutaneous modeling.

Description

technical field [0001] The invention relates to the field of establishment of a tumor transplantation mouse model, in particular to a construction method and application of a synovial sarcoma xenotransplantation mouse model. Background technique [0002] Synovial sarcoma (SS) is a highly malignant soft tissue sarcoma, accounting for about 7-10% of soft tissue sarcoma. Due to the complex origin of SS tissue, no clear risk factors, variable pathological morphology, and difficulty in dividing benign from malignant, its diagnosis, differential diagnosis, and classification have become clinical difficulties. SS is common in men aged 20-30 (male / female ratio is about 1.2:1). It mainly occurs near the large joints of the limbs, but it can occur in almost any part of the body, and SS hardly occurs in the joints, and it is also similar to normal slippery joints. Membrane tissue doesn't matter. About 80% of primary SS occurs in the hands and feet, and about 20% occurs in non-hands a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027A61K49/00
CPCA01K67/0271A61K49/0008A01K2207/12A01K2207/15A01K2227/105A01K2267/0331A01K2267/0393
Inventor 黎志宏涂超任晓磊陈瑞奇刘忠越
Owner THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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