Construction method and application of synovial sarcoma heterogenous transplanting mouse model

Abstract

The invention provides a construction method and application of a synovial sarcoma heterogenous transplanting mouse model. The method comprises the steps of firstly, collecting a synovial sarcoma patient tumor tissue block, and performing heterogenous in-situ transplanting of the fresh patient tumor tissue block into an immune deficiency mouse body under the sterile condition, wherein heterogenousin-situ planting includes the steps of selecting a 2-6-week old immune deficiency mouse, the mouse is consistent with the patient in gender, the collected fresh patient tumor tissue block is soaked with a sterile incubation solution containing fetal calf serum, the mouse is fixed after anesthesia, the patient tumor tissue block is planted into the joint capsule of the mouse, an incision is sewn,and the mouse is bred so that a tumor in the joint capsule can grow. Compared with subcutaneous modeling, the in-situ modeling condition is more real, the screened medicine scheme can be better used for the patient, and on the basis of the in-situ modeling theory, tumor metastasis is more likely to happen compared with subcutaneous modeling.

Description

technical field [0001] The invention relates to the field of establishment of a tumor transplantation mouse model, in particular to a construction method and application of a synovial sarcoma xenotransplantation mouse model. Background technique [0002] Synovial sarcoma (SS) is a highly malignant soft tissue sarcoma, accounting for about 7-10% of soft tissue sarcoma. Due to the complex origin of SS tissue, no clear risk factors, variable pathological morphology, and difficulty in dividing benign from malignant, its diagnosis, differential diagnosis, and classification have become clinical difficulties. SS is common in men aged 20-30 (male / female ratio is about 1.2:1). It mainly occurs near the large joints of the limbs, but it can occur in almost any part of the body, and SS hardly occurs in the joints, and it is also similar to normal slippery joints. Membrane tissue doesn't matter. About 80% of primary SS occurs in the hands and feet, and about 20% occurs in non-hands a...

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Problems solved by technology

The study found that the cells continuously adapt to the in vitro environment during the passage process, and the biological characteristics are not consistent with the tumor cells in the body. Defects lead to a high failure rate in clinical trials of anticancer drugs

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