Construction method of synovial sarcoma xenograft mouse model

A technology of synovial sarcoma and mouse model, applied in the field of tumor transplantation mouse model establishment, can solve the problems of high failure rate of anti-tumor drug clinical trials, lack of immune system, etc., to prevent medical social burden, easy tumor metastasis, The effect of improving effectiveness

Active Publication Date: 2019-11-26
THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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The study found that the cells continuously adapt to the in vitro environment during the passage process, and the biological character

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  • Construction method of synovial sarcoma xenograft mouse model
  • Construction method of synovial sarcoma xenograft mouse model
  • Construction method of synovial sarcoma xenograft mouse model

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[0029] After review and approval by the hospital ethics committee, all patients signed an informed consent form before sample collection. The collected tissues undergo histopathological examination. The tumor tissues of patients with synovial sarcoma confirmed pathologically are stored in sterile saline and placed on ice. on. Divide the tissue block into 3 parts. The first part is directly processed under aseptic conditions (see below) for xenotransplantation into immunodeficient mice; the second part is implanted with 10% DMSO (dimethyl sulfoxide), 90% FBS (fetal bovine serum) and 1% NEAA (non-essential amino acids) in DMEM (a medium containing various amino acids and glucose), freeze the gradient to -80 degrees, and store in liquid nitrogen; the third part is placed Quickly freeze in liquid nitrogen, and then store in liquid nitrogen. Among them, the first part is used for modeling, the second part is used for resuscitation and vaccination, and the third part is used for gen...

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Abstract

The invention provides a construction method of a synovial sarcoma xenograft mouse model. The method includes the steps that a synovial sarcoma patient's tumor tissue block preserved in a liquid nitrogen environment is recovered; then, the patient's recovered tumor tissue block is subjected to heterogenetic orthotopic transplantation into an immune defect mouse's body under the sterile condition,wherein in heterogenetic orthotopic transplantation, the immune defect mouse which is 2-6 weeks old is selected and the mouse's gender is kept consistent with the patient's gender; the patient's recovered tumor tissue block is soaked in a sterile incubation solution containing fetal calf serum, the mouse is fixed after being anesthetized, the patient's tumor tissue block is implanted into the mouse's joint capsule, the incision is sewn, and the mouse is raised to make tumors in the joint capsule grow. Compared with subcutaneous modeling, in-situ modeling is more real, and a drug scheme which is screened out can be better used in the patient; besides, theoretically, tumor metastasis can occur more easily in in-situ modeling than in subcutaneous modeling.

Description

technical field [0001] The invention relates to the field of establishing a tumor transplantation mouse model, in particular to a method for establishing a synovial sarcoma xenograft mouse model. Background technique [0002] Synovial sarcoma (SS) is a highly malignant soft tissue sarcoma, accounting for about 7-10% of soft tissue sarcoma. Due to the complex origin of SS tissue, no clear risk factors, variable pathological morphology, and difficulty in dividing benign from malignant, its diagnosis, differential diagnosis, and classification have become clinical difficulties. SS is common in men aged 20-30 (male / female ratio is about 1.2:1). It mainly occurs near the large joints of the limbs, but it can occur in almost any part of the body, and SS hardly occurs in the joints, and it is also similar to normal slippery joints. Membrane tissue doesn't matter. About 80% of primary SS occurs in the hands and feet, and about 20% occurs in non-hands and feet such as ribs, pericar...

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IPC IPC(8): A01K67/027
CPCA01K67/027A01K2227/105A01K2267/0331
Inventor 黎志宏任晓磊万璐李文逸戚麟
Owner THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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