Preparation process of sheep placenta extract and application thereof
A preparation process and sheep placenta technology, applied in the field of preparation technology of sheep placenta extract, can solve the problems of improving skin condition, single efficacy, unable to fully obtain sheep embryo high activity sheep placenta, etc., and achieve the effect of delaying aging
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Embodiment 1
[0023] A preparation process of sheep placenta extract, comprising the steps of:
[0024] (1) Wash the sheep embryo and sheep placenta in purified water to remove impurities and blood, then mince them with a meat grinder, and use ultrasonic cleaning to remove blood.
[0025] (2) Select a compound enzyme preparation consisting of trypsin, papain, lipase, neutral protease, bromelain and chymotrypsin, and add it to the ground raw materials of sheep embryos and sheep placenta according to 3% of the ground raw materials , and then adjust the pH to 3-8, and perform enzymatic hydrolysis at 30°C-60°C for 5h-6h;
[0026] (3) Add 0.1% of the mass of the stressed yeast in turn to the raw material treated with enzyme inactivation, then add 0.5% of glucose, stir and mix evenly, and ferment for 12h to 16h; After being incubated for 30 minutes, the centrifuge was centrifuged at a speed of 5000 r / min for 10 minutes, and the supernatant was collected to obtain the sheep placenta extract.
Embodiment 2
[0028] A preparation process of sheep placenta extract, comprising the steps of:
[0029] (1) Wash the sheep embryo and sheep placenta in purified water to remove impurities and blood, then mince them with a meat grinder, and use ultrasonic cleaning to remove blood.
[0030] (2) select the compound enzyme preparation that trypsin, papain, lipase, bromelain and chymotrypsin form for use, and it is added in the ground raw material of sheep embryo and sheep placenta according to 4% of ground raw material, then pH Adjust to 3~8, enzymatically hydrolyze at 30°C~60°C for 4h~7h; after enzymolysis, carry out enzyme inactivation treatment at 60°C~90°C for 30min.
[0031] (3) Add 0.15% of the mass of the stressed yeast in turn to the raw material treated with enzyme inactivation, then add 0.5% of glucose, stir and mix evenly, and ferment for 12h to 16h; After being incubated for 30 minutes, the centrifuge was centrifuged at a speed of 5000 r / min for 15 minutes, and the supernatant was ...
Embodiment 3
[0033] A preparation process of sheep placenta extract, comprising the steps of:
[0034] (1) Wash the sheep embryo and sheep placenta in purified water to remove impurities and blood, then mince them with a meat grinder, and use ultrasonic cleaning to remove blood.
[0035] (2) Select a compound enzyme preparation composed of trypsin, papain, lipase, neutral protease, bromelain and chymotrypsin, and add it to the ground raw materials of sheep embryos and sheep placenta according to 5% of the ground raw materials , and then adjust the pH to 3-8, and perform enzymatic hydrolysis at 30°C to 60°C for 6h to 8h; after enzymatic hydrolysis, carry out an enzymatic inactivation treatment at 60°C to 90°C for 40min.
[0036] (3) Add 0.15% of the mass of the stressed yeast in turn to the raw material treated with enzyme inactivation, then add 0.8% of glucose, stir and mix evenly, and ferment for 14h to 18h; After being incubated for 40 minutes, the centrifuge was centrifuged at a speed ...
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