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An electrochemical ratiometric sensor for detecting peroxynitrite anion and its preparation and application

A peroxynitroso, electrochemical technology, applied in the direction of material electrochemical variables, etc., can solve the problems of poor dispersion and limited application, and achieve the effects of low detection limit, wide detection range and strong anti-interference performance

Active Publication Date: 2021-06-29
NORTHWEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its poor dispersion in water greatly limits its application

Method used

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  • An electrochemical ratiometric sensor for detecting peroxynitrite anion and its preparation and application
  • An electrochemical ratiometric sensor for detecting peroxynitrite anion and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, the preparation of electrochemical ratiometric sensor

[0025] (1) Preparation of platinum / conjugated organic microporous material (Pt-CMP): the reactant H 2 PtCl 6, surfactant Triton-100, and hydrochloric acid were mixed and dissolved under ultrasound at a molar ratio of 1:3:5000; ascorbic acid (AA) with 0.0013 times the total molar weight of reactants was added, and the solution mixture was dissolved in a water bath under ultrasonic treatment Heated to 45°C and kept for 5h; cooled to room temperature after the reaction, and centrifuged and washed 4 times; the product was dried and ground and then mixed with conjugated organic microporous material (CMP) at a mass ratio of 1:3 to obtain platinum / conjugated organic Microporous material (Pt-CMP).

[0026] (2) Pretreatment of the glassy carbon electrode: the glassy carbon electrode is polished into a mirror surface with 0.30 μm and 0.05 μm Al2O3 suspension in turn, and then ultrasonically cleaned with 95% e...

Embodiment 2

[0028] Example 2. Electrochemical ratio sensor (Pt-CMP-CTS / GCE) detects ONOO in solution - concentration

[0029] (1) Preparation of sample solution: 1.3M H 2 o 2 and 0.1M HCl followed by adding 1.0M NaNO 2 middle. Next, the pH of the solution was adjusted to 3 and stirring was continued for 5 minutes. Then the same volume of 7M NaOH was added. Next, add 0.70g MnO 2 powder to remove excess H 2 o 2 . The product was stored at 4°C and its concentration was determined by UV-Vis absorption spectroscopy at 302.0 nm. The known concentration of ONOO - The solution was diluted to 3.51×10 -9 M.

[0030] (2) ONOO in solution - Concentration detection: the modified electrode Pt-CMP-CTS / GCE prepared in the example is used as the working electrode, the platinum column is used as the counter electrode, and the saturated calomel electrode is used as the reference electrode to form a three-electrode system. Potassium cyanide 0.2M pH=7.68 phosphate buffer solution, and use it as ...

Embodiment 3

[0031] Example 3. Electrochemical ratio sensor (Pt-CMP-CTS / GCE) detects ONOO in solution - concentration

[0032] (1) Preparation of sample solution: 1.3M H 2 o 2 and 0.1M HCl followed by adding 1.0M NaNO 2 middle. Next, the pH of the solution was adjusted to 3 and stirring was continued for 5 minutes. Then the same volume of 7M NaOH was added. Next, add 0.70g MnO 2 powder to remove excess H 2 o 2 . The product was stored at 4°C and its concentration was determined by UV-Vis absorption spectroscopy at 302.0 nm. The known concentration of ONOO - The solution was diluted to 5.68×10 -5 M.

[0033] (2) ONOO in solution - Concentration detection: the modified electrode Pt-CMP-CTS / GCE prepared in the example is used as the working electrode, the platinum column is used as the counter electrode, and the saturated calomel electrode is used as the reference electrode to form a three-electrode system. Potassium cyanide 0.2M pH=7.68 phosphate buffer solution, and use it as ...

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Abstract

The invention provides an electrochemical ratio sensor for detecting peroxynitrite anion, which is to disperse platinum / conjugated organic microporous material Pt-CMP in the acetic acid solution of chitosan, and the concentration of Pt-CMP is 0.2 ~10mg·mL ‑1 The dispersion liquid; and the dispersion liquid is drop-coated on the pretreated bare glassy carbon electrode, and dried at room temperature, and the prepared modified electrode Pt-CMP-CTS / GCE is an electrochemical ratiometric sensor. The modified electrode Pt-CMP-CTS / GCE electrode is used as the sensing platform, and peroxynitrite anion and potassium ferricyanide are used as two active substances. At a working potential of -0.2~1.0V, scan with differential pulse voltammetry; at a specific ONOO ‑ Concentration range of ONOO ‑ The response current (I ONOO ‑ ) and K 3 [Fe(CN) 6 ] The response current (I Fe ) ratio with ONOO ‑ Concentration showed a good linear relationship.

Description

technical field [0001] The invention relates to a preparation method of an electrochemical ratio sensor for detecting peroxynitrite anions. Background technique [0002] Peroxynitrite anion (ONOO - ) is produced by nitric oxide radicals (NO • ) and superoxide anion (O 2 •- ) are rapidly formed and exhibit a wide range of biochemical reactions, including (a) nitration of proteins (tyrosine residues), carbohydrates and nucleic acids, (b) oxidation of lipids, sulfhydryl groups, Fe / S and Zn / S centers, (c) Conversion of oxyhemoglobin to methemoglobin. Due to its broad reactivity, ONOO - Associated with several pathological conditions such as acute ischemia-reperfusion injury, arthritis, carcinogenesis, Alzheimer's and Parkinson's diseases, and AIDS, among others. Therefore, more and more scholars are devoted to the detection and research of peroxynitrite anion. The reported detection methods for peroxynitrite anion include oxidation of fluorescent probes, chemiluminescence...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26
CPCG01N27/26
Inventor 刘秀辉李琳张彬雁刘福鑫
Owner NORTHWEST NORMAL UNIVERSITY
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