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High-performance phospholipase A2 detection kit

A detection kit and phospholipase technology, applied in the field of biomedical testing, can solve the problems of large influence of human factors, long reaction time, and influence on accuracy

Pending Publication Date: 2019-12-03
WUHAN HANHAI NEW ENZYMES BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides a high-performance phospholipase A2 detection kit, which solves the problem that the ELISA detection method is greatly affected by human factors, and simultaneously detects phospholipas

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Examples

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Example Embodiment

[0026] Example 1

[0027] S1. Wavelength setting of the automatic biochemical analyzer: wavelength setting of the automatic biochemical analyzer: select a suitable automatic biochemical analyzer, and at the same time set the main wavelength on the automatic biochemical analyzer to 405nm and the secondary wavelength to 505nm , after the setting is completed, start the detection work;

[0028]S2. Determination of absorbance A1: Determination of absorbance A1: put 50mmol / L buffer solution, 20mmol / L inhibitor, 2g / L polyethylene glycol 6000 and 20% v / v protective agent in reagent R1 into high speed Then add the serum sample into the disperser, mix evenly at the speed of 300r / min, and incubate at 35°C for 3min after mixing evenly. After the incubation, measure the absorbance A1 and read it. Take, wait for spare;

[0029] S3. Determination of absorbance A2: Determination of absorbance A2: add 50mmol / L buffer solution, 0.05% w / v preservative, 20% v / v co-solvent and 30mmol / L substra...

Example Embodiment

[0031] Example 2

[0032] S1. Wavelength setting of the automatic biochemical analyzer: select a suitable automatic biochemical analyzer, and set the main wavelength on the automatic biochemical analyzer to 405nm and the secondary wavelength to 505nm. After the setting is completed, start the detection work;

[0033] S2, the measurement work of absorbance A1: 80mmol / L buffer solution, 25mmol / L inhibitor, 4g / L polyethylene glycol 6000 and 30% v / v protective agent in reagent R1 are put into high-speed dispersion machine, then in Add serum samples into the disperser, mix evenly at a speed of 400r / min, and incubate at 36°C for 4 minutes after mixing evenly. After the incubation is complete, measure the absorbance A1 and read it, waiting for use;

[0034] S3, the measurement work of absorbance A2: add 80mmol / L buffer solution, 0.06%w / v preservative, 25%v / v co-solvent and 40mmol / L substrate in reagent R2 to the mixed solution obtained according to S2, Continue to mix, and set the s...

Example Embodiment

[0036] Example 3

[0037] S1. Wavelength setting of the automatic biochemical analyzer: select a suitable automatic biochemical analyzer, and set the main wavelength on the automatic biochemical analyzer to 405nm and the secondary wavelength to 505nm. After the setting is completed, start the detection work;

[0038] S2, the measurement work of absorbance A1: 100mmol / L buffer solution, 30mmol / L inhibitor, 5g / L polyethylene glycol 6000 and 40% v / v protective agent in reagent R1 are put into high-speed dispersion machine, then in Add the serum sample into the disperser, mix evenly at the speed of 500r / min, and incubate at 37°C for 5 minutes after mixing evenly. After the incubation is completed, measure the absorbance A1 and read it, waiting for use;

[0039] S3, the measurement work of absorbance A2: add 100mmol / L buffer solution, 0.07%w / v preservative, 30%v / v co-solvent and 50mmol / L substrate in reagent R2 to the mixed solution obtained in S2, Continue to mix, and set the spe...

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Abstract

The invention discloses a high-performance phospholipase A2 detection kit which comprises a kit including a reagent R1 and a reagent R2. The reagent R1 is formed by a buffer solution, an inhibitor, polyethylene glycol 6000 and a protective agent, and the pH value of the reagent R1 is 7.5. The reagent R2 is formed by a buffer solution, a preservative, a cosolvent and a substrate, and the pH value of the reagent R2 is 5.5. The buffer solutions in the reagent R1 and the reagent R2 are HEPES buffer solutions, and the concentration of the buffer solutions is 50 to 100 mmol/L. The invention relatesto the technical field of biomedical examination. According to the high-performance phospholipase A2 detection kit, a whole blood sample is replaced with a serum sample, interference factors in bloodplasma can be eliminated, the treatment time of the whole blood sample is reduced, meanwhile, the inhibitor and the cosolvent are added into the detection kit, so that the reaction speed and the sensitivity of the reagents can be effectively improved, the detection kit is not easily interfered by external factors, the rapid detection can be carried out on a full-automatic biochemical analyzer, andthe actual use requirements are met.

Description

technical field [0001] The invention relates to the technical field of biomedical testing, in particular to a high-performance phospholipase A2 detection kit. Background technique [0002] Phospholipase A2 is a hydrolase that can catalyze the diacyl group on the phospholipid glycerol molecule. The lipid mediator plays a key role in the activation of membrane channels, information transmission, hemodynamics, and pathophysiological processes during inflammation and tissue damage, as well as in the regulation of intracellular and extracellular metabolism. In acute pancreatitis, septic shock, trauma PLA2 activity in the serum of patients with multiple organ failure (MSOF) increases. In multiple trauma, the change of PLA2 activity may be an early signal parameter in the process of tissue damage in MSOF. Phospholipase A2 (PLA2) is based on the Ca2+-dependent PLA2 The distribution in the body can be divided into secretory type (PLA2-I and PLA2-II) and cytoplasmic type (PLA2-III an...

Claims

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Application Information

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IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 杨广宇谢渊胡鹏高
Owner WUHAN HANHAI NEW ENZYMES BIOLOGICAL TECH CO LTD
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