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A kind of Bacillus cereus strain with anti-nematode activity and application thereof

A kind of Bacillus cereus, anti-root knot nematode technology

Active Publication Date: 2021-11-30
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the biological control of root-knot nematode has been studied for many years, there is still no significant progress in the control of root-knot nematode.

Method used

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  • A kind of Bacillus cereus strain with anti-nematode activity and application thereof
  • A kind of Bacillus cereus strain with anti-nematode activity and application thereof
  • A kind of Bacillus cereus strain with anti-nematode activity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1: Isolation, purification and identification of Bacillus cereus

[0048] Soil samples were evenly taken from the rhizosphere of the cucumber planting in Langfang, a total of six parts, 10g each, mixed evenly; 60g soil was added to 1000mL distilled water, stirred evenly, let stand for 1min, and the supernatant was taken at 80°C Water bath for 30 minutes, fully and stir when heating, so that the heat is even, let stand for 15 minutes, cool to room temperature, shake well, take 1mL supernatant, dilute 100 times with sterilized water, and then take 0.25mL of the diluted liquid and spread on LB plate (yeast powder 5g / L, peptone 10g / L, NaCl 10g / L, agar powder 1.3-1.5g / 100mL, dilute to 1L, seal. 121℃, 20min, high temperature and high pressure sterilization, in a petri dish Spread it on a plate), smear it evenly, place it upside down in a constant temperature incubator at 28°C for 2-4 days, and check the size of the colony every day. After the tissue blocks grew co...

Embodiment 2

[0056] Embodiment 2: the nematocidal effect of bacillus cereus Bc-cm103 bacterial strain fermented liquid

[0057] 1. Preparation of Bacillus cereus Bc-cm103 strain fermentation broth

[0058] Bacillus cereus Bc-cm103 strain (concentration of 10 6 cfu / ml) was inoculated into a 500mL Erlenmeyer flask containing 100mL of LB liquid medium, cultured with shaking (220rpm) at 28°C for 2 days, and the fermentation broth and its 10-fold and 100-fold dilutions were respectively treated with root-knot nematodes (Meloidogyne spp .) Second-instar larvae, to determine the lethal effect of fermentation products of Bacillus cereus on root-knot nematodes.

[0059] 2. Preparation of nematodes for experiment

[0060] Root-knot nematodes were collected from the greenhouse of the Vegetable and Flower Research Institute of the Chinese Academy of Agricultural Sciences. Take out the root system of the root-knot nematode-infested pepper, rinse it gently with water, carefully remove the egg mass fr...

Embodiment 3

[0068] Example 3: Isolation, Identification and Nematicidal Effect of Nematicidal Substances of Bacillus cereus Bc-cm103 Strain

[0069] 3-1. Preparation of nematicidal substances

[0070] a. Liquid fermentation of Bacillus cereus Bc-cm103 strain

[0071] Activation of Bacillus cereus Bc-cm103 strain. LB solid medium: Yeast powder 5g / L, peptone 10g / L, NaCl 10g / L, agar powder 1.3-1.5g / 100mL, purified water 1000mL, autoclaved at 121°C for 30 minutes. LB liquid medium: yeast powder 5g / L, peptone 10g / L, NaCl 10g / L, purified water 1000mL, sterilized by high pressure steam at 121°C for 30 minutes. The Bacillus cereus Bc-cm103 strain stored in the -80°C refrigerator was spread on the LB plate by the coating method, and cultured overnight at 37°C. A single colony of Bacillus cereus Bc-cm103 grown on the LB plate was picked and inoculated in LB liquid medium, and cultivated overnight at 37°C to obtain the strain of Bacillus cereus Bc-cm103.

[0072] Liquid fermentation of Bacillus ...

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Abstract

The invention provides a Bacillus cereus strain with anti-root-knot nematode activity and application thereof. The strain is Bacillus cereus strain Bc-cm103, and its preservation number is CGMCCNO.17580. The strain was isolated from the rhizosphere soil of Ceratia japonica. The present invention provides a new strain of Bacillus cereus Bc-cm103, which has high anti-root-knot nematode activity, has a good control effect on root-knot nematode, is safe in application, and is suitable for Biological control of root-knot nematodes. The invention obtains three anti-root-knot nematode compounds by fermenting the Bacillus cereus Bc-cm103 strain, and the compounds have high lethality to the root-knot nematode.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a bacillus cereus strain with anti-nematode activity and application thereof. Background technique [0002] Root-knot nematode is an important plant parasitic nematode with a wide range of hosts and serious damage. It is one of the important diseases in agricultural production. Root-knot nematodes can infest as many as 3,000 plant species and cause severe economic losses to plants. With the adjustment of planting structure and methods in northern my country, the occurrence of root-knot nematode diseases is becoming more and more common and serious under the environmental conditions of protected cultivation. Therefore, root-knot nematode seriously threatens normal agricultural production. The most common are M. incognita, M. javanica, M. arenaria, and M. hapla. [0003] The harm of root-knot nematodes infecting plants is mainly manifested in two aspects: on t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P13/02A01N63/22A01N37/18A01P5/00C07C233/05C12R1/085
CPCA01N37/18A01N63/00C07C233/05C12P13/02C12N1/205C12R2001/085
Inventor 茆振川李彦谢丙炎凌键殷楠杨宇红赵建龙
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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