A liquid chromatography-mass spectrometry screening method for multi-target antithrombotic active substances

A screening method and anti-thrombotic technology, applied in the field of liquid chromatography-mass spectrometry screening, can solve the problems of affecting the sensitivity of mass spectrometry, increasing operating steps, increasing reagent costs, etc., to save analysis time, strong anti-interference ability, and save reagent costs Effect
CN110568117BActive Publication Date: 2022-03-22MARINE BIOMEDICAL RES INST OF QINGDAO CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
MARINE BIOMEDICAL RES INST OF QINGDAO CO LTD
Publication Date
2022-03-22

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Abstract

The invention discloses a liquid chromatography-mass spectrometry screening method for multi-target antithrombotic substances based on dried blood spot samples, which belongs to the field of drug screening. The method comprises the following steps: rehydrating the dried blood spot sample, optionally several substrates of key target enzymes involved in the thrombus formation process, mixing several substrate standard solutions with the substances to be screened and then adding them to the rehydrated In the dried blood spot sample, the reaction solution is processed and analyzed by liquid chromatography-mass spectrometry to screen for substances with multi-target antithrombotic activity. The screening method of the present invention is simple and quick to operate, has high accuracy and strong specificity, and can continuously analyze a large number of samples. The target of substance action is clear, which lays a theoretical foundation for the subsequent drug development. The reagents used are easy to obtain, the degree of operation automation is high, clinical promotion and popularization are easy, and the commercial value is high.
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Description

technical field

[0001] The invention relates to the field of drug screening, in particular to a liquid chromatography-mass spectrometry screening method for multi-target antithrombotic active substances based on dried blood spot samples. Background technique

[0002] According to reports, stroke, coronary heart disease and other thrombotic diseases have become the main killers threatening human health. Studies have shown that abnormal activation of platelets leads to platelet aggregation and a series of coagulation factors are activated successively to convert fibrinogen into fibrin, and then form a network and recruit red blood cells to rapidly increase the thrombus mass, and finally combine tightly with platelets to form a firm thrombus , which is the main cause of arterial thrombotic disease and venous thrombotic disease. From the perspective of thrombus formation mechanism, a variety of enzymes and receptors are involved in the process of thrombus formation. Theoretica...

Claims

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