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Mesenchymal stem cells and exosomes of mesenchymal stem cells and application of mesenchymal stem cells in treating anal fistula

A technology of mesenchymal stem cells and exosomes, applied in the field of stem cells, can solve problems such as differences in curative effects and unstable cell survival rates

Pending Publication Date: 2019-12-24
沈阳细胞治疗工程技术研发中心有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Stem cell therapy has made some progress, the healing rate has been significantly improved, and the recurrence rate has been reduced. However, due to the unstable cell survival rate, the curative effect is different. Therefore, it is necessary to provide a good living environment for the injected cells, improve the cell survival rate, and ensure a stable therapeutic effect.
[0004] Although the use of stem cells to treat anal fistula has been reported at home and abroad, but the use of mesenchymal stem cell exosomes and its preparations for the treatment of anal fistula has not yet been reported.

Method used

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  • Mesenchymal stem cells and exosomes of mesenchymal stem cells and application of mesenchymal stem cells in treating anal fistula
  • Mesenchymal stem cells and exosomes of mesenchymal stem cells and application of mesenchymal stem cells in treating anal fistula
  • Mesenchymal stem cells and exosomes of mesenchymal stem cells and application of mesenchymal stem cells in treating anal fistula

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Embodiment 1

[0023] A mixed preparation for treating anal fistula, comprising the following active ingredients: mesenchymal stem cells (ADSCs), exosomes of mesenchymal stem cells (EXO-ADSCs), autologous platelet-rich plasma (PRP) and synthetic antibacterial polypeptide (AMP).

[0024] The preparation method of the described PRP comprises the following steps: extracting 35ml of peripheral blood from the patient, adding 8ml of anticoagulant, transferring to a 50ml centrifuge tube, centrifuging at 300g / min for 25min, absorbing the upper plasma, middle buffy coat and a small amount of red blood cells to the In a new 50ml centrifuge tube; then centrifuge at 750g / min for 10min, suck out the supernatant to a new centrifuge tube for later use, use the supernatant to adjust the platelet concentration to 800×10 6 Each / mL is PRP.

[0025] The method for extracting exosomes from mesenchymal stem cells comprises the following steps: take the culture supernatant of the amplified third-generation mesench...

Embodiment 2

[0031] A mixed preparation for treating anal fistula, comprising the following active ingredients: mesenchymal stem cells (ADSCs), exosomes of mesenchymal stem cells (EXO-ADSCs), autologous platelet-rich plasma (PRP) and synthetic antibacterial polypeptide (AMP).

[0032] The preparation method of the described PRP comprises the following steps: extracting 50ml of peripheral blood from the patient, adding 2ml of anticoagulant, transferring to a 50ml centrifuge tube, centrifuging at 300g / min for 30min, absorbing the upper plasma, the middle buffy coat and a small amount of red blood cells to the In a new 50ml centrifuge tube; then centrifuge at 700g / min for 10min, suck out the supernatant to a new centrifuge tube for later use, use the supernatant to adjust the platelet concentration to 1000×10 6 Each / mL is PRP.

[0033] The method for extracting exosomes from mesenchymal stem cells comprises the following steps: take the culture supernatant of the amplified third-generation me...

Embodiment 3

[0039] A mixed preparation for treating anal fistula, comprising the following active ingredients: mesenchymal stem cells (ADSCs), exosomes of mesenchymal stem cells (EXO-ADSCs), autologous platelet-rich plasma (PRP) and synthetic antibacterial polypeptide (AMP).

[0040] The preparation method of described PRP comprises the following steps: extracting 20ml of peripheral blood from the patient himself, adding 10ml of anticoagulant, transferring to a 50ml centrifuge tube, centrifuging at 300g / min for 20min, absorbing the upper plasma, the middle buffy coat and a small amount of red blood cells to the In a new 50ml centrifuge tube; then centrifuge at 800g / min for 10min, suck out the supernatant to a new centrifuge tube for later use, use the supernatant to adjust the platelet concentration to 500×10 6 Each / mL is PRP.

[0041] The method for extracting exosomes from mesenchymal stem cells comprises the following steps: take the culture supernatant of the amplified third-generatio...

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Abstract

The invention discloses mesenchymal stem cells and exosomes of the mesenchymal stem cell and application of the mesenchymal stem cells in treating anal fistula, and further discloses a method for treating anal fistula by using an autologous-fat-derived mesenchymal stem cell mixed preparation. The stem cell preparation includes autologous-adipose-derived mesenchymal stem cells (ADSCs) and exosomes(EXO-ADSCs) of the ADSCs, autologous platelet-rich plasma (PRP) and synthetic bacteriostatic peptides (AMP). The stem cells have immunoregulatory functions, the exosomes create a micro-environment which is conducive to recovery, meanwhile, the stem cells are stimulated to release various growth factors, and wound healing is promoted. PROP gel provides support and nutrients and works synergistically with stem cells, the survival rate after stem cell transplantation is improved, and the stem cells can better play a role. The synthetic peptides are single in components and controllable and reducethe incidence of infection. The combined use greatly improves the cure rate of anal fistula.

Description

technical field [0001] The present invention relates to the technical field of stem cells, in particular to a mesenchymal stem cell and its exosome and its application in the treatment of anal fistula. Background technique [0002] Anal fistula is a common complication of inflammatory bowel disease (IBD). It refers to the formation of a conduit between the rectum and the perianal skin. It is affected by multiple factors such as genetic factors, immune regulation, and intestinal flora, resulting in long-term non-healing of the fistula. Conventional treatment has a large wound, is prone to infection, and is prone to recurrence. [0003] Stem cell therapy has made some progress, the healing rate has been significantly improved, and the recurrence rate has been reduced. However, due to the unstable cell survival rate, the curative effect is different. Therefore, it is necessary to provide a good living environment for the injected cells to improve the cell survival rate and ensu...

Claims

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Application Information

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IPC IPC(8): C12N5/0775A61K38/02A61P1/00A61K35/28A61K35/16
CPCC12N5/0667C12N5/0662A61K35/28A61K35/16A61K38/02A61P1/00A61K2300/00
Inventor 张宇荣耀星于艳秋杨柳松
Owner 沈阳细胞治疗工程技术研发中心有限公司
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