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Application of epimedin B in preparation of medicine for treating dopaminergic neuron damage

A neuron injury, dopaminergic technology, applied in the fields of cell biology and pharmacology, can solve the problems of unreported effects, achieve the goal of inhibiting neuron apoptosis, improving motor coordination ability, and reducing dopaminergic neuron apoptosis Effect

Inactive Publication Date: 2019-12-27
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Other uses and effects of Epimedin B have not been reported

Method used

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  • Application of epimedin B in preparation of medicine for treating dopaminergic neuron damage
  • Application of epimedin B in preparation of medicine for treating dopaminergic neuron damage
  • Application of epimedin B in preparation of medicine for treating dopaminergic neuron damage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1, MPTP prepares PD model mouse

[0032] 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (1-methyl1-4-phenvl-1,2,3,6-tetrahvdropvridine, MPTP) is a commonly used preparation of Parkinson's disease model medicine. The drug itself has no toxic effect on cells, but when it enters the central nervous system through the blood-brain barrier and acts on monoamine oxidase B (MAO-B) in astrocytes, it can rapidly generate pyridine metabolite 1-methyl- 4-Phenyl-pyridinium ion (MPP + ), this metabolite has toxic effects on dopaminergic neurons in the substantia nigra of the midbrain. It can inhibit the mitochondrial electron transport chain complex I, resulting in insufficient mitochondrial ATP production, decreased membrane potential and dysfunction, thereby increasing the production of oxygen free radicals, causing cell damage, and initiating apoptosis. This results in a decrease in the number of dopaminergic neurons in the substantia nigra of the midbrain, leading to ...

Embodiment 2

[0043] Example 2. Behavioral experiments to detect the effect of different concentrations of Epimedin B on improving the movement disorder of PD model mice

[0044] Normal mice have the ability to climb, grasp, and coordinate movements of limbs. In the novel environment, the mice showed exploratory behavior and autonomous activities. In this embodiment, the rotarod test and pole climbing test were used to detect the motor coordination ability of the mice, and the open field test was used to detect the autonomous activities of the mice.

[0045] (1) Rotating rod experiment

[0046] The rotarod test is a method used to evaluate the motor coordination ability of mice. Mice were balanced on a rotarod roller and moved continuously. After the administration of Example 1, the mice in each group on the first 1-2 days underwent adaptive learning for 2-3 times, and the training was carried out 30 minutes before gavage, with a rotational speed of 2-20 r / min, and each training was perf...

Embodiment 3

[0062] Example 3. Western blotting assay to detect the effects of different concentrations of Epimedin B on the expression of TH, Bax and Bcl-2 proteins in the substantia nigra of PD model mice

[0063] 1. Materials and methods

[0064] Rabbit primary anti-Bcl-2 antibody, purchased from Cell signaling company, the ratio is 1:1000;

[0065] Rabbit primary anti-Bax antibody, purchased from Cell signaling company, the ratio is 1:1000;

[0066] Mouse primary anti-β-actin antibody: purchased from Bioss Company, the ratio is 1:8000;

[0067] Rabbit primary anti-TH antibody: purchased from Millipore, the ratio is 1:1000;

[0068] Anti-rabbit secondary antibody: purchased from Tianjin Sanjian Biotechnology Co., Ltd., with a ratio of 1:5000;

[0069] Anti-mouse secondary antibody: purchased from Tianjin Sanjian Biotechnology Co., Ltd., with a ratio of 1:5000.

[0070] The substantia nigra tissue homogenate of each group of mice was centrifuged to obtain the supernatant. Take 1 μL ...

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Abstract

The invention belongs to the fields of cytobiology and pharmacology, and relates to an application of a natural medicine herba epimedii extract epimedin B, in particular to an application of epimedinB in preparation of a medicine for treating dopaminergic neuron damage. Expression of apoptotic protein is promoted by increase of dopaminergic neurons in brain substantia nigra due to dopaminergic neuron damage, and meanwhile, expression of tyrosine hydroxylase and anti-apoptotic protein is reduced. Epimedin B has remarkable anti-apoptotic activity, can be used for preparing medicines for preventing and / or treating neuronal apoptosis and related diseases thereof. The application has higher clinical application value and development prospects.

Description

technical field [0001] The invention belongs to the field of cell biology and pharmacology, and relates to the application of epimedin B, an extract of epimedium, a natural medicine, and in particular to the application of epimedin B in the preparation of medicines for treating dopaminergic neuron damage. Background technique [0002] Parkinson's disease (PD) is a degenerative disease of the central nervous system characterized by the progressive degeneration and loss of dopaminergic neurons in the substantia nigra pars compacta and the formation of Lewy bodies. Motor symptoms such as tremor, bradykinesia, muscle rigidity, and postural disturbance, and non-motor symptoms such as hyposmia, insomnia, and constipation. Parkinson's disease is a serious brain disease that endangers the health of the elderly, and is known as the "third killer" that threatens middle-aged and elderly people. With the aging of the population, the incidence of Parkinson's disease is increasing year b...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P25/16A61P25/00
CPCA61K31/7048A61P25/00A61P25/16
Inventor 张梅陈文芳
Owner QINGDAO UNIV
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