Virginiamycin monoclonal antibody hybridoma cell strain YSL and application thereof

Abstract

The invention discloses a virginiamycin monoclonal antibody hybridoma cell strain YSL and application thereof, and belongs to the field of food safety immunodetection. The produced virginiamycin monoclonal antibody hybridoma cell strain YSL is preserved in China General Microbiological Culture Collection Center, the preservation date is March 7, 2019, and the preservation number is CGMCC No.17397.According to the virginiamycin monoclonal antibody hybridoma cell strain YSL and application thereof, by conducting mixing emulsification on a complete antigen of virginiamycin and the same amount ofFreund's adjuvant, conducting multi-point injection immunization on BALB / c mice, then screening out cells through an indirect competitive euzymelinked immunosorbent assay method and conducting subcloning for three times, the monoclonal antibody hybridoma cell strain YSL is obtained finally. A monoclonal antibody secreted by the provided cell strain YSL has good specificity and detection sensitivity (an IC50 value is 40 ng / mL) for VIR, the residual amounts of VIR in chicken, livers of chickens and feed of the chickens can be detected, and a raw material is provided for immunodetection of residual VIR in food, so that the virginiamycin monoclonal antibody hybridoma cell strain YSL has practical application values.

Description

technical field [0001] The invention relates to a virginiamycin monoclonal antibody hybridoma cell line YSL and an application thereof, belonging to the field of food safety immunoassay. Background technique [0002] Virginiamycin (Virginiamycin, VIR), also known as pure mycin, can only inhibit Gram-positive bacteria. The mechanism of action is to inhibit the bacterial ribosome (ribosome) and prevent it from protein synthesis to achieve a bactericidal effect. After oral administration, it only stays and acts on the intestinal tract, and is mainly excreted in feces with a small residual amount. Oral LD50 for rats is 7500 mg per kilogram of body weight, once orally, when the dose for mice reaches 1550 mg per kilogram of body weight, no adverse reactions have been found, and the maximum safe dose for pigs is 800 mg per kilogram of body weight. The dose of 500 mg was continuously fed to pigs for 3 months without adverse reactions. [0003] Virginiamycin has been widely used i...

AI Technical Summary

Problems solved by technology

However, these standards are all detected by high-performance liquid chromatography, which is cumbersome and complicated. In order to protect the interests of consumers, it is necessary to establish an efficient and rapid detection method for VIR, and enzyme-linked immunoassay (ELISA) Simple pre-treatment, low cost, rapid detection of a large number of samples, and low requirements for sample purity

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