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Method for preparing hovenia dulcis thunnb., hovenia acerba lindl. and hovenia trichocarpa chun et tsiang mature seed extract by enzymolysis method

A technology of Hovenia dulcis fruit and extract, which is applied in the field of preparing Hovenia dulcis fruit extract by enzymatic hydrolysis, which can solve the problems of unfriendly environment, high cost, and impossibility of direct application, and achieve easy dissolution, mild process conditions, and great application value Effect

Inactive Publication Date: 2020-01-07
SHANGHAI NOVANAT BIORESOURCES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented inventions use bio-enzymes from plants called bacteria or fungi for making extraction products like auranthodies (a type of herb known commonly found throughout India). These methods have been developed over time but they still require expensive processes involving high temperatures, chemical reagents, and complicated equipment. However these techniques help break down complex structures inside vegetables without damaging their nutritional content.

Problems solved by technology

The technical problem addressed in this patents is how to efficiently prepare certain types of herbs called Harrow' s Mining Bone Tree(HB) Decay White Spotting Ptermaceae Root Black Lipsilodes Fruttae).

Method used

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  • Method for preparing hovenia dulcis thunnb., hovenia acerba lindl. and hovenia trichocarpa chun et tsiang mature seed extract by enzymolysis method
  • Method for preparing hovenia dulcis thunnb., hovenia acerba lindl. and hovenia trichocarpa chun et tsiang mature seed extract by enzymolysis method
  • Method for preparing hovenia dulcis thunnb., hovenia acerba lindl. and hovenia trichocarpa chun et tsiang mature seed extract by enzymolysis method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048]Take 1kg Hovenia dulcis (purchased from Hunan Songlingtang Chinese Herbal Pieces Co., Ltd., after testing, the total flavonoid content is 2.4%), make a coarse powder (through a 10-mesh sieve), put it in an extraction tank, add 10L of reverse osmosis to drink Pure water, start stirring, heat up to 48-52 ℃, add 0.2g cellulase+0.1g papain (purchased from Shanghai Ruji Biotechnology Development Co., Ltd., cellulase activity 300,000 IU / g, papain activity 20 10,000 IU / g), extract for 5h. After the extraction, pass through a 200-mesh filter bag to filter out most of the residue, and the filtrate is centrifuged in a high-speed tubular centrifuge (model: Bosheng GQ75RS), and the speed is adjusted to 7000r / min to obtain the centrifugal supernatant, which is at 58-60 At ℃, keep the vacuum at -0.09-0.095Mpa, concentrate to a solid content of 25% feed liquid 608g, take it out, at 58-60℃, keep the vacuum at -0.09-0.095Mpa, vacuum dry to get 152g of yellow Hovenia dulcis fruit extract...

Embodiment 2

[0054] Take 5kg Hovenia dulcis (purchased from Hunan Songlingtang Chinese Herbal Pieces Co., Ltd., after testing, the total flavonoid content is 2.4%), make a coarse powder (through a 10-mesh sieve), put it in an extraction tank, and add 50L of reverse osmosis to drink Pure water, start stirring, heat up to 48-52°C, add 1g pectinase + 0.5g bromelain (purchased from Shanghai Ruji Biotechnology Development Co., Ltd., pectinase activity 300,000 IU / g, bromelain activity 50,000 IU / g), extract for 5h. After the extraction is completed, pass through a 200-mesh filter bag to filter out most of the residue, and the filtrate is centrifuged in a high-speed tubular centrifuge (model: Bosheng GQ75RS), and the speed is adjusted to 8000r / min to obtain the centrifugal supernatant. At ℃, keep the vacuum degree at -0.09-0.095Mpa, concentrate to 2480g of solid content 29.8% liquid, take it out, at 58-60℃, keep the vacuum degree at -0.09-0.095Mpa, vacuum dry to obtain 740g yellow Hovenia dulcis ...

Embodiment 3

[0060] Take 10kg Hovenia dulcis (purchased from Hunan Songlingtang Chinese Herbal Pieces Co., Ltd., after testing, the total flavonoid content is 2.4%), make coarse powder (through a 10-mesh sieve), put it in an extraction tank, add 100L of reverse osmosis to drink Purified water, start stirring, heat up to 48-52°C, add 2g hemicellulase+1g glucosidase (purchased from Shanghai Ruji Biotechnology Development Co., Ltd., hemicellulase activity 200,000 IU / g, bromelain activity 100,000 IU / g), extract for 5h. After the extraction is completed, pass through a 200-mesh filter bag to filter out most of the residue, and the filtrate is centrifuged in a high-speed tubular centrifuge (model: Bosheng GQ75RS), and the speed is adjusted to 10000r / min to obtain the centrifugal supernatant, which is at 58-60 At a temperature of -0.09-0.095Mpa at a temperature of -0.09-0.095Mpa, it is concentrated to a solid content of 32.2% and 4560g of the feed liquid is taken out. At a temperature of 58-60°C,...

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Abstract

The invention provides a method for preparing a hovenia dulcis thunnb., hovenia acerba lindl. and hovenia trichocarpa chun et tsiang mature seed extract by an enzymolysis method. The method comprisesthe steps of adding deionized water to hovenia dulcis thunnb., hovenia acerba lindl. and hovenia trichocarpa chun et tsiang mature seed powder, performing stirring, adding composite enzymes, performing extraction at 50-60 DEG C, and performing filtering; filtering filtrate through a high speed centrifugation machine at 60 DEG C, and performing vacuum concentration at 58-60 DEG C; and performing vacuum drying on a concentrated solution to obtain the hovenia dulcis thunnb., hovenia acerba lindl. and hovenia trichocarpa chun et tsiang mature seed extract being good in dissolubility. A biologicalenzymolysis technique is adopted, so that plant cell walls can be selectively destroyed, digesting of effective components is facilitated, the clarity of the extract is greatly improved, and the recovery rate of total flavone in the hovenia dulcis thunnb., hovenia acerba lindl. and hovenia trichocarpa chun et tsiang mature seeds can be notably increased. The hovenia dulcis thunnb., hovenia acerbalindl. and hovenia trichocarpa chun et tsiang mature seed extract product can be widely applied to foods, beverages and health-care foods, and is good in practicality. The method is simple to operateand mild in technology condition, cost is greatly saved, industrial production is easy to realize, and the method has high application value.

Description

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Claims

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Application Information

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Owner SHANGHAI NOVANAT BIORESOURCES CO LTD
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