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Application of isorhamnetin in preparing medicine for inhibiting pulmonary artery smooth muscle cell proliferation

The technology of isoindolinone and compound is applied in the application field of isoindolinone compounds in the preparation of medicines for preventing and treating osteolytic diseases, which can solve the problems of increased bone fragility, carcinogenesis and the like, and achieve the effect of great clinical application value.

Active Publication Date: 2020-01-10
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, the drug treatment of osteolytic diseases mainly includes drugs that inhibit bone resorption, drugs that promote bone formation, and basic supplements. However, bisphosphonates, the first-line drugs that inhibit bone resorption, excessively promote bone mineralization, and long-term use also leads to bone mineralization. Increased fragility; and bone-promoting drugs are often accompanied by adverse reactions such as palpitations and cancer

Method used

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  • Application of isorhamnetin in preparing medicine for inhibiting pulmonary artery smooth muscle cell proliferation
  • Application of isorhamnetin in preparing medicine for inhibiting pulmonary artery smooth muscle cell proliferation
  • Application of isorhamnetin in preparing medicine for inhibiting pulmonary artery smooth muscle cell proliferation

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Embodiment 1

[0028] In this example, to explore the inhibitory activity of isoindolinone compounds on osteoclast differentiation, the following tests were carried out.

[0029] (1) Cell culture: bone marrow-derived macrophage BMMs were isolated from C57BL / 6 femur and tibia, cultured in a medium containing 10% fetal bovine serum, streptomycin (100 μg / mL), penicillin (100 units / ml) In α-MEM medium, routine maintenance culture and subculture were carried out at 37°C and 5% carbon dioxide concentration.

[0030] (2) TRAP staining experiment: BMMs cells were plated in a 96-well plate at a density of 3×103 / well, and after waiting for 24 hours for the cells to adhere to the wall, the drug was added to test the effect of different concentrations of isoindolinone compounds on RANKL (50ng / well). mL) and MCSF (30ng / mL) induced osteoclast formation, and the osteoclasts were stained with the TRAP kit, and the osteoclasts (TRAP + The number of cells with nuclei ≥ 3) reflects the inhibitory activity of ...

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Abstract

The invention discloses an application of isorhamnetin in preparation of a medicine for inhibiting pulmonary artery smooth muscle cell proliferation. The experiment results show that the isorhamnetincan be used in a safe dosage range; 100 [mu] M of aloperine can significantly inhibit the proliferation of human pulmonary artery smooth muscle cells, and significantly adjusts the expression of BMP signal pathway related proteins, so that isorhamnetin inhibits pulmonary vascular remodeling, and further inhibits pulmonary hypertension, cardiac blood abnormal tubes or chronic obstructive pulmonarydiseases.

Description

technical field [0001] The invention relates to the technical field of compounds and medicines, in particular to the application of isoindolinone compounds in the preparation of medicines for preventing and treating osteolytic diseases. Background technique [0002] Osteolytic disease refers to a systemic bone disease in which bone density and bone quality decrease and bone microstructure is destroyed due to the increase in the number and activity of osteoclasts, resulting in increased bone fragility and prone to fracture. At present, a large number of literatures have shown that the functional filling of osteoclasts mediated by RANKL is closely related to various types of pathological osteolysis, such as: bone destruction caused by tumors, inflammatory osteolysis, aseptic osteolysis after artificial joint replacement, etc. Sexual prosthesis loosening, postmenopausal osteoporosis, Page's disease, psoriatic arthritis and ankylosing spondylitis, etc. With the advent of the gl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4035A61P19/08A61P19/10A61P17/06
CPCA61K31/4035A61P19/08A61P19/10A61P17/06
Inventor 顾琼佘志刚华沛崔辉蔡润林
Owner SUN YAT SEN UNIV