Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A tremella fungus strain and its application in the production of tremella polysaccharide

A technology of tremella polysaccharides and tremella strains, applied in the fields of applications, fungal products, fungi, etc., can solve the problems of single molecular size of polysaccharides, high content of protein impurities, single molecular weight of products, etc., and achieve easy control conditions, easy separation and purification, The effect of a short growth cycle

Active Publication Date: 2021-07-09
NANJING TECH UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that the fermentation method has many problems such as low polysaccharide yield, high protein impurity content, single molecular size of the output polysaccharide, and high input-output ratio.
Therefore, it is imperative to screen and obtain a high-yield strain for fermenting tremella polysaccharides, and to solve the problems of high input cost and single molecular weight of the breakthrough product.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A tremella fungus strain and its application in the production of tremella polysaccharide
  • A tremella fungus strain and its application in the production of tremella polysaccharide
  • A tremella fungus strain and its application in the production of tremella polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] figure 1 As shown in (a), the Tremella fungus strain CGMCC No.17675 was smeared in PDA solid medium (glucose agar medium), and cultured at a constant temperature of 26°C for 5 days to obtain a single colony (such as figure 1 Shown in (b)) and its 18s sequence was sequenced, and after Blast comparison, it was determined to be a Tremella fungus strain. The 18s sequence is shown in the sequence listing SEQ ID No.1.

Embodiment 2

[0059] Embodiment 2: the preparation of fermented liquid

[0060] The preparation method of a single colony is the same as in Example 1.

[0061] Solid-state fermentation culture of high-yield strains: Pick the obtained strains into the seed medium (glucose 25g / L, soybean peptone 2g / L, MgSO 4 0.5g / L, KH 2 PO 4 0.46g / L, K 2 HPO 4 1g / L, pH 6.0), 26°C, 170rpm constant temperature culture for 3 days. Inoculate in solid medium (glucose 15g / L, MgSO 4 0.5g / L, KH 2 PO 4 0.5g / L, K 2 HPO 4 0.5g / L, bran 8g, soybean meal powder 12g, relative humidity 80 ± 5%), cultured at 26°C for 6-8 days. The yield of Tremella polysaccharides was 12.9g / L as detected by the sulfate anthrone method, of which the large molecular weight was 1000-1500kDa, accounting for 54% of the total polysaccharides, and the small molecular weight was 100-300kDa, accounting for 46% of the total polysaccharides.

Embodiment 3

[0062] Embodiment 3: the preparation of fermented liquid

[0063] The preparation method of a single colony is the same as in Example 1.

[0064] Solid-state fermentation culture of high-yield strains: Pick the obtained strains into the seed medium (glucose 25g / L, soybean peptone 2g / L, MgSO 4 0.5g / L, KH 2 PO 4 0.46g / L, K 2 HPO 41g / L, pH 6.0), 26°C, 170rpm constant temperature culture for 3 days. Inoculate in solid medium (glucose 15g / L, (NH 4 ) 2 SO 4 3g / L, MgSO 4 0.5g / L, KH 2 PO 4 0.5g / L, K 2 HPO 4 0.5g / L, bran 8g, soybean meal 12g, relative humidity 80±5%), cultured at 26°C for 6-8 days. According to the anthrone sulfate method, the yield of tremella polysaccharides is 22.5g / L, of which the large molecular weight is 1000-1500kDa, accounting for 75% of the total polysaccharides, and the small molecular weight is 100-300 kDa, accounting for 25% of the total polysaccharides.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
molecular weightaaaaaaaaaa
Login to View More

Abstract

In the present invention, a Tremella fuciformis strain (Tremella fuciformis) was screened out, the strain name is XY, and it was deposited in the General Microbiology Center of the China Microbiological Culture Collection Management Committee. The preservation date is April 25, 2019, and the preservation number is CGMCC No.17675. The present invention also provides the application of the above Tremella fungus strains in the production of Tremella polysaccharides, the main process of which is to insert the seed liquid prepared from CGMCC No. 17675 into a solid-state fermentation medium for fermentation to obtain a fermentation liquid. It can prepare high-molecular-weight polysaccharides with a molecular weight of 1000-1500kDa and low-molecular-weight polysaccharides with a molecular weight of 100-300kDa. The total output can reach 15-25g / L, and the protein content is less than 0.3%. Compared with the liquid fermentation method, it has a significant cost advantage. Tremella It has a good application prospect in the industrial production of polysaccharides.

Description

technical field [0001] The invention belongs to the field of microorganisms and fermentation engineering, and relates to a tremella fungus strain and its application in the production of tremella polysaccharides. Background technique [0002] Tremella, also known as white fungus, belongs to the subclass Tremellaceae, which has high nutritional value and medicinal efficacy. Tremella, as a special product of my country, is planted in many provinces such as Zhejiang, Fujian, and Hubei. As the main production area of ​​tremella, Fujian Province has reached more than 90% of the national total output. Tremella contains carbohydrates, proteins, vitamins and a variety of amino acids, and the most important biologically active substance is Tremella polysaccharide, which can be obtained by direct extraction or fermentation from Tremella fruiting bodies. It has immune regulation, anti-tumor, Anti-oxidative aging, lowering blood sugar and blood lipids, anti-coagulation and thrombus, an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01H15/00C12P19/04C12R1/645
CPCA01H15/00C12N1/14C12P19/04C12N1/145C12R2001/645
Inventor 李莎葛辛燕徐虹雷鹏续晓琪
Owner NANJING TECH UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com