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A method for detecting maize stalk strength and its specific transposon

A technology of transposon and corn stalk, applied in the field of method and its specific transposon, detecting the strength of corn stalk

Active Publication Date: 2021-03-23
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And in theory, taller crops will have higher biomass and yield if they are not lodging, while the lower biomass of dwarf crops will eventually become a bottleneck for high yield

Method used

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  • A method for detecting maize stalk strength and its specific transposon
  • A method for detecting maize stalk strength and its specific transposon
  • A method for detecting maize stalk strength and its specific transposon

Examples

Experimental program
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Effect test

Embodiment 1

[0067] Example 1. Discovery of a transposon in the promoter region of the corn stalk strength gene Stiff1 and its correlation with stalk strength

[0068] 1. Discovery of a transposon in the promoter region of the maize stalk strength gene Stiff1

[0069] The research found that compared with the Kill inbred line, in the Stiff1 gene (sequence 9) promoter region (sequence 1) of the typical hard stalk inbred line B73, there is a -1094-bp upstream from the start codon. 27.3 kb Ty1 / Copia transposon insertion.

[0070] The nucleotide sequence of the 27.3kb Ty1 / Copia transposon is No. 2156-29434 of Sequence 1 in the Sequence Listing.

[0071] Taking the Ki11 inbred line as a standard, the sequence of the Ki11stiff1 promoter region is the DNA molecule shown in Sequence 2 in the sequence table; it does not contain the transposon, and its hardness is not as high as that of B73.

[0072] 2. Correlation analysis of 27.3kb Ty1 / Copia transposon insertion and stem strength

[0073] A pop...

Embodiment 2

[0098] Example 2. Application of the 27.2 kb Ty1 / Copia transposon in the promoter region of the maize stalk strength gene Stiff1

[0099] 1. Application of the 27.2kb Ty1 / Copia transposon in the promoter region of the corn stalk strength gene Stiff1

[0100] 1. Transposon-specific primers identify the stalk strength of maize to be tested

[0101] The corn inbred line 52220 (https: / / plants.usda.gov / core / profile?symbol=ZEMA), the corn inbred line 178 (Wei et al., A new allele of the Brachytic2 gene in maizecan efficiently modify plant architecture.Heredity,121,75-86(2018).), zheng58 and chang72(Wei et al.,A new allele of the Brachytic2 gene in maize can efficiently modify plant architecture.Heredity,121,75-86(2018).) of genomic DNA.

[0102]Using the genomic DNA of each of the above inbred lines as a template, PCR amplification was performed with the above-mentioned I1, I2 and I3 primers.

[0103] If primer I1 has amplified products, and primers I2 and I3 have no amplified pr...

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Abstract

The invention discloses a method for detecting the stalk strength of maize and a specific transposon thereof. The invention provides application of matter for detecting a maize gene Stiff1promoter region whether contains a 27.2-Kb Ty1 / Copia transposon or not in identification or assisting identification the stalk strength of to-be-detected maize, or, application of the matter for detecting the maize gene Stiff1promoter region whether contains the 27.2-Kb Ty1 / Copia transposon or not in preparation identification and assisting identification of the stalk strength of the to-be-detected maize. Thenucleotide sequence of the 27.2-Kb Ty1 / Copia transposon is a 2156th-29434th site in a sequence 1. According to the method and the application, it is found that the gene Stiff1promoter region containsa 27.3-Kb Ty1 / Copia transposon, and the transposon is related to the stalk strength; and therefore, the stalk strength of the maize can be determined by detecting the transposon in the gene Stiff1 promoter region.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for detecting the strength of corn stalks and a specific transposon. Background technique [0002] In the 1870s, with the extensive use of chemical fertilizers in agriculture, the semi-dwarf gene was widely used around the world, and the lodging resistance of crops was greatly improved, which doubled the yield of rice and corn at that time. Effectively avoided a potential food crisis. In subsequent studies, it was found that the semi-dwarf genes that were widely used at that time were all related to gibberellin (GA), that is, Semi Dwarf-1 (SD-1) in rice and Reduced height-1 (Rht -1), the disappearance or weakening of the gibberellin signal can cause the reduction of plant height without causing other unfavorable morphological and physiological traits. Therefore, the model of reducing plant height through gibberellin-related genes to improve lodging resistance of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11C12N15/113
CPCC12N15/8226C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 林中伟
Owner CHINA AGRI UNIV