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Single strain of brevundimonas sp.2B and application thereof

A technology of brevundimonas and bacteria, applied in the direction of application, bacteria, biochemical equipment and methods, etc., can solve problems such as drug residues and environmental pollution that cannot be effectively avoided, shorten the production cycle, increase the thickness of the cap, and increase the economy benefit effect

Active Publication Date: 2020-01-14
QUFU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For the deficiencies in the prior art, the technical problem to be solved by this invention is to provide a strain of bacteria Brevundimonas 2B that promotes the growth of Pleurotus ostreatus, which is a suitable edible fungus growth-promoting bacterium; another technical problem to be solved by this invention is to provide The application of Brevundimonas 2B in promoting the growth of Pleurotus ostreatus to solve the problems in the prior art that drug residues and environmental pollution cannot be effectively avoided while promoting the growth of fungi

Method used

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  • Single strain of brevundimonas sp.2B and application thereof
  • Single strain of brevundimonas sp.2B and application thereof
  • Single strain of brevundimonas sp.2B and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0024] 1) Sample collection and storage

[0025] The soil samples of different fruiting bodies, fungus bags, and soil-covered edible fungi were randomly collected at the planting base of Edible Fungi Science and Technology Industrial Park in Zhuyang Town, Tai'an, Shandong Province. All soil samples were collected by aseptic operation and put into sterile bags after collection. Store at ℃.

[0026] 2) Purification and preservation of bacteria

[0027] Accurately weigh 1g of the soil sample to be tested, put it into a sterilized test tube filled with 9mL of sterile water, place it on a shaker for 20min, set the shaker at 28°C, and rotate at 170r / min to disperse the microbial cells, and let it stand for 20- 30s, Serve 10 -1 diluent; then use a 1mL sterile pipette to draw 10 -1 Dilute 1mL, transfer it into a test tube filled with 9mL sterile water, blow and aspirate the bacterial solution, mix evenly, and serve as 10 -2 Diluent; change another sterile pipette to draw 10 -2 Di...

Embodiment 2

[0043] Application of Pleurotus ostreatus Growth Promoting Bacteria

[0044] The strain of Pleurotus ostreatus was selected from Tai'an Edible Fungi Research Institute, and was cultivated in the solar greenhouse of the edible fungi base. The temperature and water content in the base are moderate, which is suitable for the growth of normal Pleurotus ostreatus strains.

[0045] Embodiment Three groups of repeated bacteria addition groups and one group of control groups were set up.

[0046] Put the mixed compost into a 23cm×36cm polyethylene bag by machine bagging method, each bag is filled with 2kg of compost, then put a collar on one end of the bag and then seal it with a plastic film, and extinguish it at 100°C under normal pressure Bacteria 8h. After sterilization, the control group was inoculated when the material temperature dropped to normal temperature naturally.

[0047] Inoculation group A was first inoculated with Brevundimonas 2B bacteria liquid, and then inoculat...

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Abstract

The invention discloses a single strain of brevundimonas sp.2B and application thereof, and relates to the technical field of edible mushroom biology. A classification name of the bacteria is brevundimonas sp.2B, and the bacteria is preserved in China center for type culture collection, of which the preservation number is CCTCC No: M 2019638, the preservation date is August, 16th, 2019, and the preservation address is Wuhan University, Wuhan province, China. The bacteria of the brevundimonas sp.2B is applied to growth promotion of oyster mushrooms, daily growth speed of mycelium, yield of single bag and thickness of pileus are increased, the days of full bag of the mycelium is shortened, growth of the mycelium of the oyster mushrooms can be effectively promoted, the production period of the oyster mushrooms is shortened, the problems of residues of chemical pesticides and environmental pollution can be effectively avoided while the growth of fungi is promoted at the same time, and development and application of the brevundimonas sp.2B in the business field can be facilitated in the future.

Description

technical field [0001] The invention relates to the field of edible fungus biotechnology, in particular to a strain of Brevundimonas 2B and its application. Background technique [0002] Pleurotus otreatus, also known as Pleurotus otreatus, Oyster Mushroom, Black Peony Mushroom, is a species of Pleurotaceae under the Basidiomycota Agaricaceae. It is a fairly common gray edible mushroom and is deeply loved by consumers. Pleurotus ostreatus is rich in nutrients, and every 100 grams of oyster mushroom contains 20-23 grams of protein, and the amino acid composition and variety are complete. The taste of Pleurotus ostreatus is delicious because it contains many kinds of amino acids, which can stimulate people's taste organs to produce a sense of umami taste, which is much better than the umami taste of monosodium glutamate contained in monosodium glutamate, and it will not cause Thirst phenomenon. Studies have shown that flat mushrooms also contain physiologically active substa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01G18/00C12R1/01
CPCA01G18/00C12R2001/01C12N1/205
Inventor 李冠喜张璐于颖媛焦玉茹李洁李慧琳程振豪郭帅
Owner QUFU NORMAL UNIV
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