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Recombinant turkey herpesvirus strain for expressing chicken infectious bursal disease virus VP2 gene

A technology of turkey herpes virus, chicken infectivity, in the field of molecular biology and genetic engineering vaccines

Pending Publication Date: 2020-01-14
YEBIO BIOENG OF QINGDAO
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, due to the interference of maternal antibodies and the characteristics of IBDV, an inevitable immune blank period is formed in the practical application of traditional vaccines.

Method used

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  • Recombinant turkey herpesvirus strain for expressing chicken infectious bursal disease virus VP2 gene
  • Recombinant turkey herpesvirus strain for expressing chicken infectious bursal disease virus VP2 gene
  • Recombinant turkey herpesvirus strain for expressing chicken infectious bursal disease virus VP2 gene

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Experimental program
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Embodiment 1

[0030] 1 Experimental materials

[0031] 1.1 Strains, strains and plasmids

[0032] The HVT FC-126 vaccine strain was preserved by Qingdao Yibang Bioengineering Co., Ltd.

[0033] IBDV was isolated in the field by Qingdao Yibang Bioengineering Co., Ltd.

[0034] Competent cells Trans-T1 were purchased from Beijing Quanshijin Biotechnology Co., Ltd.; chicken embryo fibroblast cell line (DF-1) was preserved by Qingdao Yibang Bioengineering Co., Ltd.

[0035] Plasmids: pEASY-T3, pEASY-Blunt, and pEASY-M1 were purchased from Beijing Quanshijin Biotechnology Co., Ltd.; PCR2.1 and pT-EGFP were preserved by Qingdao Yibang Bioengineering Co., Ltd., where pT-EGFP contains green fluorescent protein (GFP) expression cassette, with Not I and Avr II restriction sites at both ends of the expression cassette.

[0036] 2 Experimental methods

[0037] 2.1 Construction of intermediate transfer vector pFR

[0038] 2.1.1 Design of homology arm primers

[0039] The UL45-UL46 spacer of HVT wa...

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Abstract

The invention provides a VP2 gene recombinant turkey herpesvirus strain for expressing a chicken infectious bursal disease virus variant. The VP2 gene recombinant turkey herpesvirus strain is preparedby inserting an IBDV VP2 gene into a turkey herpesvirus genome. The recombinant virus rHVT-vVP2 strain is obtained by inserting an expression cassette containing a VP2 gene into the turkey herpesvirus genome by taking a turkey herpesvirus (HVT) FC-126 vaccine strain as a carrier and taking a spacer region of UL45-UL46 of the turkey herpesvirus (HVT) FC-126 vaccine strain as an insertion site. Theimmune protection evaluation results show that after chickens are immunized by the rHVT-vVP2 strain, 100% virus attacking protection can be provided for attacks of IBDV virulent strains and variant strains.

Description

technical field [0001] The invention belongs to the technical field of molecular biology and genetic engineering vaccines, and in particular relates to a recombinant turkey herpes virus strain expressing the VP2 gene of chicken infectious bursal disease virus. The recombinant virus can be used to immunize poultry for chicken infectious bursal disease Virus prevention. Background technique [0002] Marek's disease (MD) and infectious bursal disease (IBD) are two common immunosuppressive diseases affecting poultry performance. In the 1960s, the turkey herpesvirus (herpesvirus of turkey, HVT) FC-126 strain was used as a vaccine to control the occurrence of MD, and it has been widely used worldwide, greatly reducing the incidence of MD. economic loss. As the virulence of MDV increases, the original vaccines can no longer effectively prevent and control it, and new and more effective vaccines have been continuously developed. However, HVT vaccines are still widely used, especia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/01C12N15/40A61K39/12A61P31/14C12R1/93
CPCC12N7/00C07K14/005A61K39/12A61P31/14C12N2720/10022C12N2720/10034C12N2710/16021
Inventor 侯玉超楚电峰孙鹏孙化露李振于晓璐范根成杜元钊
Owner YEBIO BIOENG OF QINGDAO
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