Chicken infectious bursal disease virus VP2 protein and preparation method and application thereof and kit

Abstract

The invention provides chicken infectious bursal disease virus VP2 protein and a preparation method and application thereof and a kit. Particularly, a nucleotide sequence for coding the VP2 protein contains the sequence as shown in SEQ ID No.1. The method for preparing the VP2 protein comprises the following steps of chemically synthesizing the SEQ ID No.1 sequence, constructing an expression vector and transfecting sf9 cells. The VP2 protein can be used for preparing a vaccine for a chicken infectious bursal disease. The SEQ ID No.1 is obtained by conducting codon optimization on a chicken IBDV VP2 gene according to insect cell preference codon, and therefore compared with a general VP2 gene which is amplified from an IBDV virus, the SEQ ID No.1 is more suitable for being expressed in insect cells and can increase the protein expression quantity.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a chicken infectious bursal disease virus VP2 protein, its preparation method, application and kit. Background technique [0002] Chicken infectious bursal disease (Infectious Bursal Disease, IBD) is an acute, highly contagious infectious disease caused by infectious bursal virus (Infectious Bursal Disease Virus, IBDV), mainly against chicks and young chickens Bursa of Fabricius. The disease not only causes the death of chickens but also leads to immunosuppression, which is one of the main infectious diseases endangering the poultry industry in the world. Infectious bursal disease virus is a double-stranded double-segment dsRNA virus, and its genome is composed of two double-stranded RNA segments, A and B. The length of fragment A is 3200bp, and the length of fragment B is about 2800bp. Fragment A has two open reading frames, the large ORF1 encodes the three proteins VP2, VP...

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Problems solved by technology

[0003] There are many ways to express VP2 gene in exogenous systems, such as prokaryotic expression system, which has a high expression level, but the product is often an inclusion body and has no biological activity; the glycosylation modification of the expression product by the yeast expression system is incomplete; and Although the mammalian cell expression system can modify the expression product such as glycosylation to produce an active protein, its expression level is low, it is not easy to scale up, and the cell growth conditions are relatively high.

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