Primers, kit and identifying method for simultaneously and rapidly identifying 4 types of taenia solium larvae

Abstract

The present invention provides primers for simultaneously and rapidly identifying 4 types of taenia solium larvae of cysticercus cellulosae, taenia asiatica cysticercus, cysticercus tenuicollis and echinococcus granulosus, and also provides a corresponding detection kit and a detection method. The designed specific primers for the taenia solium larvae can quickly identify 4 types of the widely prevalent taenia solium larvae in China in one PCR reaction system, overcome a problem that insect bodies are similar in forms and cannot be accurately identified by morphology, and also overcome a defect of piece weight PCR identification of a single species, besides, PCR products do not need sequencing and sequence alignment, molecular identification of the 4 types of the taenia solium larvae can be realized only according to sizes of electrophoretic fragments of the PCR products, which greatly shortens time of identification diagnosis, and the primers can be used for rapid identification diagnosis of the 4 types of taenia solium larvae and epidemiological investigation of metacestodiasis.

Description

technical field [0001] The invention belongs to the technical field of microbial determination and inspection methods, and in particular relates to primers, kits and identification methods for rapidly identifying four kinds of Taenia larvae at the same time, capable of rapidly identifying cysticerci and Taenia saginata cysts in the same PCR reaction system cercariae, cysticercosis and echinococcus granulosus. Background technique [0002] Taenia solium, Taenia asiatica, Taenia hydatigena and Echinococcus granulosus are all tapeworms of the family Taeniidae. The middle stage larvae (cysticerci or echinococcosis) of Taeniaceae are commonly known as tapeworm larvae. Cysticercus porcine (cysticercosis), cysticercus asiatica, echinococcosis granulosus and cysticercus cerevisiae are all tapeworm larvae parasitic in pigs, and they can all parasitize in the liver of pigs. Cysticercus and Taeniasis cysticercosis) and waste of internal organs (Echinococcosis granulosus), and Cystice...

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Problems solved by technology

[0003] In order to solve the problems existing in the morphological identification of the existing tapeworm larvae, the present invention provides a multiplex method for the differential diagnosis of 4 kinds of tapeworm larvae (cysticercus porcine, cysticercus asiatica, cysticercus lentinococcus and echinococcus granulosus) PCR primers, reagents and identification methods, PCR amplification in the same reaction system, PCR products do not need to be sequenced, only through the difference in the size of the amplified fragments of different species of tapeworm larvae, the species of tapeworm larvae infected can be quickly identified, overcoming the morphological The defects of the single-plex PCR identification of single species and single species provide a strong technical guarantee for the epidemiological investigation and traceability of swine tapeworm larvae

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