Biological mixed artificial blood vessel and preparation method thereof

An artificial blood vessel and hybrid technology, which is applied in the field of bioengineering to achieve the effects of facilitating angiogenesis, promoting endothelial repair, and regulating angiogenesis

Active Publication Date: 2020-02-07
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current artificial blood vessels still have shortcomings in the repair and immune regulation after damage. Therefore, it is necessary to develop an artificial blood vessel with good biocompatibility, which is conducive to blood vessel repair and immune regulation.

Method used

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  • Biological mixed artificial blood vessel and preparation method thereof
  • Biological mixed artificial blood vessel and preparation method thereof
  • Biological mixed artificial blood vessel and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The preparation of embodiment 1 biohybrid artificial blood vessel

[0040] 1. Preparation of Inner Main Body Vascular Stent

[0041] 1) Preparation of natural polymer artificial blood vessels by electrospinning: prepare a PCL electrospinning solution with a concentration of 10 g / ml with hexafluoroisopropanol, and stir until completely dissolved; the instrument used in the experiment is a YFSP-T high-voltage electrospinning machine . Put the electrospinning solution in a 10ml syringe, select the No. 7 needle, set the voltage to 15KV, the advancing speed of the syringe pump is 1ml / h, the receiving distance is 15cm, the drum receiving device is connected to a 3mm steel pipe for collection, and the drum speed is 150r / h min, the obtained polymer tubular fiber polymer is used as the inner main body vascular stent;

[0042] Prepared by the acellular matrix method:

[0043] 2) The prepared allogeneic acellular matrix stent is derived from the pig's cardiovascular system. Aft...

Embodiment 2

[0049] Example 2 Artificial blood vessels promote the growth and activity of vascular endothelial cells

[0050] ① Preparation of HUVECs culture and extract

[0051] HUVECs were cultured in RPMI1640 with 1% double antibody (penicillin / streptomycin) and 10% FBS serum at 37°C and 5% CO2, and the state of the cells was observed regularly for medium replacement or passage.

[0052] Peracetic acid was diluted with sterile PBS to a final concentration of 0.1% (v / v). It is best to use it now. Cut the prepared Gelatin, GelMA, and PCL materials into 1cm×1cm size, soak in peracetic acid for 1h. Pour off the peracetic acid, carefully rinse the material with sterile PBS, shake to wash off the peracetic acid, wash 3 times, 1 min each time. Soak in sterile PBS for 3 hours, discard the PBS, and finally soak in sterile PBS and store at 4°C. According to ISO10993-12:2012(2012), the ratio of the surface area of ​​the material to the extraction medium is 6cm 2 / ml, the material was soaked i...

Embodiment 3

[0055] Example 3 Artificial blood vessels promote the migration of vascular endothelial cells

[0056] The effects of Glatin, GelMA and PCL materials on cell migration were observed by scratch method. Digest cells in logarithmic growth phase to make 1x10 5 cells / ml cell suspension, inoculate 12-well plate with 1ml / well; when the HUVECs in the well grow to a single layer, starve the cells with serum-free medium for 4 hours; then use a 10ml pipette tip to mark "ten" on the well plate After adding PBS to wash for 3 times, add medium and extract, randomly select 6 fields of view to take pictures under the microscope, and record it as the scratch width of 0h; cultivate in 37℃, 50% COZ incubator for 12h and 36h respectively Take pictures to observe the migration of cells. Such as Figure 4 As shown, compared with the control group, the GelMA and PCL groups significantly promoted the migration of vascular endothelial cells after culturing for 12h and 36h. Therefore, it is enough t...

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Abstract

The invention belongs to the technical field of bioengineering, and particularly relates to a biological mixed artificial blood vessel, a preparation method thereof and an application of Sca-1<+> vascular stem cells in preparation of the biological mixed artificial blood vessel. The biological mixed artificial blood vessel is divided into an inner layer and an outer layer, an inner layer main bodyintravascular stent is prepared from a polymer material PCL through electrostatic spinning or through an acellular matrix method, heparinization is conducted on a blood vessel main body in a covalentbinding mode, cell infiltration and angiogenesis are facilitated, and the risk of thrombosis is reduced; and the outer layer is GelMA hydrogel encapsulating Sca-1<+> vascular stem cells, and the hydrogel has good cell adhesion, so that the cells are not easy to fall off. The artificial blood vessel can help blood vessel repair and promote endothelialization when the blood vessel is damaged. The Sca-1<+> vascular stem cells on the outer membrane are generally firstly activated, and the Sca-1<+> vascular stem cells differentiated into endothelial cells influence the structure and tension of thevascular wall and can participate in endothelial repair, vascular regeneration, immunoregulation and the like.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, in particular to a biological hybrid artificial blood vessel and its preparation method and Sca-1 + Application of vascular stem cells in the preparation of biohybrid artificial blood vessels. Background technique [0002] According to the latest report data of "China Cardiovascular Disease Report 2018", the prevalence and mortality of cardiovascular disease in China are still on the rise. The number of patients with cardiovascular diseases is as high as 290 million, and the mortality rate of cardiovascular diseases ranks first, higher than that of tumors and other diseases, accounting for more than 40% of the residents' disease deaths. Especially in rural areas, the mortality rate of cardiovascular diseases has continued to higher than the city. As the number of patients increases, the clinical demand for vascular grafts is increasing in China. The demand for peripheral vascular injury...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/07
CPCA61F2/07A61F2002/072A61F2240/001
Inventor 王贵学刘宛灵尹铁英
Owner CHONGQING UNIV
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