Preparation method of oral animal bifidobacterium microcapsule
A technology of animal bifidobacteria and microcapsules, which is applied in the field of pharmaceutical manufacturing, can solve the problems of gastric acid resistance, bile salts and poor enteric solubility, so as to improve the body's immunity, inhibit potential harmful pathogenic bacteria, and improve tolerance Effects of gastric acid and bile salts
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Embodiment 1
[0027] An oral preparation method of animal bifidobacterium microcapsules, through preparation of animal bifidobacterium suspension, water phase preparation, oil phase preparation, water-in-oil emulsion preparation, solidification, centrifugal washing and freeze drying Step, complete the preparation method of oral animal bifidobacterium microcapsules; the specific steps are as follows:
[0028] (1) Preparation of Bifidobacterium animalis suspension:
[0029] a. Activation and fermentation of bacteria
[0030] The bacteria stored in the refrigerator were inoculated onto the solid medium, cultured anaerobic in a 37°C incubator for 22 hours, and a single colony was selected to inoculate the liquid medium, and cultured in a 37°C incubator for 22 hours, the activated bacteria Seeds were inoculated into liquid culture medium with 1% inoculum, and cultivated for 15h at 37℃;
[0031] b. Centrifuge the cultured bacterial solution under high-speed rotation, discard the supernatant, and resuspe...
Embodiment 2
[0039] An oral preparation method of animal bifidobacterium microcapsules, through preparation of animal bifidobacterium suspension, water phase preparation, oil phase preparation, water-in-oil emulsion preparation, solidification, centrifugal washing and freeze drying Step, complete the preparation method of oral animal bifidobacterium microcapsules; the specific steps are as follows:
[0040] (1) Preparation of Bifidobacterium animalis suspension:
[0041] a. Activation and fermentation of bacteria
[0042] Inoculate the bacteria stored in the refrigerator on the solid culture medium, culture in a 37℃ incubator for 24 hours, select a single colony to inoculate the liquid culture medium, culture in the 37℃ incubator for 24 hours, and activate the good bacteria. Seeds were inoculated into liquid culture medium with 2% inoculum, and cultivated at 37℃ for 24h;
[0043] b. Centrifuge the cultured bacterial liquid under high-speed rotation, discard the supernatant, and resuspend in steri...
Embodiment 3
[0051] An oral preparation method of animal bifidobacterium microcapsules, through preparation of animal bifidobacterium suspension, water phase preparation, oil phase preparation, water-in-oil emulsion preparation, solidification, centrifugal washing and freeze drying Step, complete the preparation method of oral animal bifidobacterium microcapsules; the specific steps are as follows:
[0052] (1) Preparation of Bifidobacterium animalis suspension:
[0053] a. Activation and fermentation of bacteria
[0054] Inoculate the bacteria stored in the refrigerator on the solid culture medium, culture in the 37℃ incubator for 23 hours, select a single colony to inoculate the liquid culture medium, culture in the 37℃ incubator for 23 hours, the activated bacteria Seeds are inoculated into liquid culture medium with 1%-2% inoculum, and cultivated at 37℃ for 19h;
[0055] b. Centrifuge the cultured bacterial liquid under high-speed rotation, discard the supernatant, and resuspend in sterile sa...
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