Preparation method of oral animal bifidobacterium microcapsule

A technology of animal bifidobacteria and microcapsules, which is applied in the field of pharmaceutical manufacturing, can solve the problems of gastric acid resistance, bile salts and poor enteric solubility, so as to improve the body's immunity, inhibit potential harmful pathogenic bacteria, and improve tolerance Effects of gastric acid and bile salts

Inactive Publication Date: 2020-02-07
JIANGSU HFQ BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the microcapsules prepared by many current methods have poor resistance to gastric acid, bile salts and enteric solubility.

Method used

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  • Preparation method of oral animal bifidobacterium microcapsule
  • Preparation method of oral animal bifidobacterium microcapsule
  • Preparation method of oral animal bifidobacterium microcapsule

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] An oral preparation method of animal bifidobacterium microcapsules, through preparation of animal bifidobacterium suspension, water phase preparation, oil phase preparation, water-in-oil emulsion preparation, solidification, centrifugal washing and freeze drying Step, complete the preparation method of oral animal bifidobacterium microcapsules; the specific steps are as follows:

[0028] (1) Preparation of Bifidobacterium animalis suspension:

[0029] a. Activation and fermentation of bacteria

[0030] The bacteria stored in the refrigerator were inoculated onto the solid medium, cultured anaerobic in a 37°C incubator for 22 hours, and a single colony was selected to inoculate the liquid medium, and cultured in a 37°C incubator for 22 hours, the activated bacteria Seeds were inoculated into liquid culture medium with 1% inoculum, and cultivated for 15h at 37℃;

[0031] b. Centrifuge the cultured bacterial solution under high-speed rotation, discard the supernatant, and resuspe...

Embodiment 2

[0039] An oral preparation method of animal bifidobacterium microcapsules, through preparation of animal bifidobacterium suspension, water phase preparation, oil phase preparation, water-in-oil emulsion preparation, solidification, centrifugal washing and freeze drying Step, complete the preparation method of oral animal bifidobacterium microcapsules; the specific steps are as follows:

[0040] (1) Preparation of Bifidobacterium animalis suspension:

[0041] a. Activation and fermentation of bacteria

[0042] Inoculate the bacteria stored in the refrigerator on the solid culture medium, culture in a 37℃ incubator for 24 hours, select a single colony to inoculate the liquid culture medium, culture in the 37℃ incubator for 24 hours, and activate the good bacteria. Seeds were inoculated into liquid culture medium with 2% inoculum, and cultivated at 37℃ for 24h;

[0043] b. Centrifuge the cultured bacterial liquid under high-speed rotation, discard the supernatant, and resuspend in steri...

Embodiment 3

[0051] An oral preparation method of animal bifidobacterium microcapsules, through preparation of animal bifidobacterium suspension, water phase preparation, oil phase preparation, water-in-oil emulsion preparation, solidification, centrifugal washing and freeze drying Step, complete the preparation method of oral animal bifidobacterium microcapsules; the specific steps are as follows:

[0052] (1) Preparation of Bifidobacterium animalis suspension:

[0053] a. Activation and fermentation of bacteria

[0054] Inoculate the bacteria stored in the refrigerator on the solid culture medium, culture in the 37℃ incubator for 23 hours, select a single colony to inoculate the liquid culture medium, culture in the 37℃ incubator for 23 hours, the activated bacteria Seeds are inoculated into liquid culture medium with 1%-2% inoculum, and cultivated at 37℃ for 19h;

[0055] b. Centrifuge the cultured bacterial liquid under high-speed rotation, discard the supernatant, and resuspend in sterile sa...

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Abstract

The invention discloses a preparation method of an oral animal bifidobacterium microcapsule, which comprises the steps of preparation of an animal bifidobacterium suspension, preparation of a water phase, preparation of an oil phase, preparation of water-in-oil emulsion, solidification, centrifugal washing and freeze-drying. A preparation technology is simple and efficient; the prepared animal bifidobacterium microcapsule can resist gastric acid and bile salt well and is good in enteric solubility; and quick reproduction can be achieved in an intestinal tract. The animal bifidobacterium microcapsule is not disintegrated in a stomach after oral administration and smoothly reaches small intestine finally; bifidobacteria are tightly combined with intestinal epithelial cells through competitive antagonism and grow and reproduce in the intestinal tract; normal physiological bacteria are directly supplemented; potential harmful pathogenic bacteria in the intestinal tract are inhibited; florabalance of the intestinal tract is adjusted; and the body immunity is improved.

Description

Technical field [0001] The invention relates to the field of pharmaceutical manufacturing, in particular to a method for preparing oral animal bifidobacterium microcapsules. Background technique [0002] Probiotics, defined by British scholar Fuller in 1989 as: a kind of microbial additives that play a beneficial effect on the host by regulating and improving the intestinal microecological balance. Probiotics have attracted wide attention because of their superior physiological functions. [0003] Studies have found that probiotics need to consume 10 6 -10 7 g -1 At the same time, its activity will also be affected by the viability of the bacteria, acidic environment, oxygen, temperature and other factors. Therefore, scientists have proposed many methods to maintain the activity of probiotics, and microcapsules are recognized as the most efficient way to maintain probiotics. Methods of bacterial viability. [0004] However, the microcapsules prepared by many methods now have poor re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/50A61K47/36A61K35/745C12N1/20C12R1/01
CPCA61K9/5036A61K35/745C12N1/20
Inventor 阮晓洋廖洪袁芳
Owner JIANGSU HFQ BIO TECH CO LTD
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