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Preparation method of probiotic microcapsules for feed

A technology of probiotics and microcapsules, applied in the field of preparation of probiotics capsules, can solve the problems of poor stomach environment tolerance, unfavorable industrialization and application of microcapsule products, destruction of lactic acid bacteria, etc., so as to inhibit the growth of pathogenic bacteria and improve animal feeding. Feeding effect, the effect of enhancing gastric acid resistance

Inactive Publication Date: 2018-05-11
黑龙江益菌生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] To sum up, the existing microcapsule coating technology will cause greater damage to lactic acid bacteria during its implementation, or the microcapsules produced have poor tolerance to the gastric environment, which is not conducive to Industrialization and application of microcapsule products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The specific breeding process of Lactobacillus plantarum tlj-2014 of the present invention is as follows:

[0034] 1. DES mutagenesis selection

[0035] 1) Take the L-ring of Lactobacillus plantarum on the inclined surface of the test tube on the ultra-clean bench, insert it into a 250mL Erlenmeyer flask filled with 50mL medium MRS (no agar, glucose 20g / L), and culture at 200rpm at 37°C for about 12h. Make the bacteria in the early logarithmic growth stage.

[0036] 2) Take 5 mL of the bacterial liquid, centrifuge at 5000 rpm for 10 minutes to collect the bacterial cells, and wash twice with normal saline.

[0037] 3) Dilute to 10 with pH7.0 phosphate buffer 7 individual / mL bacterial suspension.

[0038] 4) Take 32mL of pH7.0 potassium phosphate buffer, 8mL of bacterial suspension, and 0.4mL of DES in a 150mL Erlenmeyer flask pre-placed in the rotor and mix thoroughly so that the final concentration of DES is 1% (v / v).

[0039] 5) React in a shaker at 37°C at 150rpm...

Embodiment 2

[0062] A preparation method of probiotic microcapsules, the probiotic microcapsules are composed of wall material, plantaractobacillus, stachyose, hickory chick enzymolysis powder, the method for coating the probiotic microcapsules is as follows: Add 3% stachyose and 8% hickory chick enzymolysis powder to the fermentation liquid of Lactobacillus plantarum CGMCC NO.9405 after fermentation and cultivation in the fermenter, and then mix it with the wall material solution to obtain a mixed solution; Vacuum spray drying method, the first level of vacuum drying, the second level of inert protective gas plus vibrating fluidized bed drying, and microencapsulation of probiotics.

[0063] The method of ultrasonic spray drying adopts the method disclosed in the invention patent application 201110415081.8. Specifically, to turn on the ultrasonic spray drying equipment, after the vacuum pressure in the drying chamber is maintained at 2.5-3.0kPa, adjust the flow rate to 0-20mL / min, and the ...

Embodiment 3

[0074] A preparation method of probiotic microcapsules, the probiotic microcapsules are composed of wall material, plantaractobacillus, stachyose, hickory chick enzymolysis powder, the method for coating the probiotic microcapsules is as follows: Add 5% stachyose and 5% hickory chick enzymolysis powder to the fermentation liquid of Lactobacillus plantarum CGMCC NO.9405 after fermentation and cultivation in the fermenter, and then mix it with the wall material solution to obtain a mixed solution; Vacuum spray drying method, the first level of vacuum drying, the second level of inert protective gas plus vibrating fluidized bed drying, and microencapsulation of probiotics.

[0075] The composition of the wall material solution is as follows: enzymatically hydrolyzed soybean protein isolate 10%, chitosan 0.5%, pullulan 0.5%, xanthan gum 0.2%, carrageenan 0.5%, glycerin 2%, trehalose 0.2%, The balance is distilled water; the above are weight and volume percentages.

[0076] Indepe...

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Abstract

The invention provides a preparation method of probiotic microcapsules. The probiotic microcapsule is composed of wall material, Lactobacillus plantarum, stachyose, and Morchella enzymolysis powder. The method for coating the probiotic microcapsule product is as follows: adding 3-5% stachyose and 5-8% morel enzymolysis powder to the probiotic fermented liquid after fermentation in the fermenter , and then the fermented liquid is mixed with the wall material solution to obtain a mixed solution; the method of ultrasonic vacuum spray drying is adopted, first-level vacuum drying, second-level inert protective gas plus vibration fluidized bed drying, and microcapsule coating of probiotics. The capsule of the invention adopts modified soybean protein isolate and has good enteric solubility. After the capsule reaches the intestinal tract, it can completely disintegrate and release probiotic bacteria within 1-1.5 hours, and rapidly proliferate into a dominant flora, thereby inhibiting the growth of pathogenic bacteria. and so on. In the present invention, the enzymatic hydrolysis powder of Morchella is used, so that the product has both the nutritional properties of Morchella, has good palatability, and improves the feeding effect of animals.

Description

technical field [0001] The invention relates to a preparation method of probiotic capsules, belonging to the technical field of microbial feed additives. Background technique [0002] Lactic acid bacteria microecological feed additives are an important alternative to antibacterial drugs. Lactic acid bacteria are one of the outstanding representatives of beneficial bacteria in the normal intestinal flora, and have important physiological and health functions: they can antagonize pathogenic microorganisms, regulate the balance of animal digestive tract microflora: activate the immune system, enhance immunity, prevent various The occurrence of diseases and adverse reactions; inhibit the occurrence of tumors, protect the health of animals; synthesize nutrients, produce digestive enzymes, improve the activity of animal digestive enzymes, improve the metabolism of vitamins, neutralize enterotoxins, and reduce the harmful substances such as amines and ammonia. produce etc. Howeve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K20/189
Inventor 邵素英
Owner 黑龙江益菌生物科技有限公司
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