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Phenol-degrading bacterium immobilization spherical granules and preparation method and application thereof

A spherical particle, phenol-reducing bacteria technology, applied in chemical instruments and methods, fixed on/in organic carriers, water pollutants, etc., can solve the problem of low mechanical strength, low pollutant degradation performance and cycle performance, Bacteria leakage and other issues

Active Publication Date: 2020-02-07
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after the agar hydrogel is formed into balls, the pore size is too large, which is prone to bacterial leakage, and on the other hand, its mechanical strength is low; therefore, the degradation performance and cycle performance of pollutants after it is loaded with microorganisms are not high.

Method used

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  • Phenol-degrading bacterium immobilization spherical granules and preparation method and application thereof
  • Phenol-degrading bacterium immobilization spherical granules and preparation method and application thereof
  • Phenol-degrading bacterium immobilization spherical granules and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Preparation of immobilization solution

[0040] 2% agar + 2% carrageenan: Weigh 2g agar and 2g carrageenan and mix, dissolve in distilled water, make up to 100mL, sterilize at 121°C for 30min, cool to 40-45°C after sterilization.

[0041] 2. Enrichment culture of reducing phenolic bacteria

[0042] Use an inoculation loop to pipette the depressor bacteria preserved in the 3-ring solid medium into a conical flask containing 100mL sterilized MPYE liquid medium, mix well, seal the mouth of the conical flask with a sealing film, and place it in a shaker. In the bed, the temperature is 30°C, the rotation speed is 200r / min, and the cultivation is carried out for 24 hours to obtain the enriched culture solution of reducing phenolic bacteria.

[0043] 3. Preparation of phenolic bacteria concentrate

[0044] Centrifuge the depletion bacteria enriched culture solution in a high-speed centrifuge at a speed of 8000r / min for 5 minutes, discard the supernatant, collect the bacte...

Embodiment 2

[0048] 1. Preparation of immobilization solution

[0049] 1.5% agar + 1.5% carrageenan: Weigh 1.5g agar and 1.5g carrageenan and mix, dissolve in distilled water, dilute to 100mL, sterilize at 121°C for 30min, cool to 40-45°C after sterilization.

[0050] 2. Enrichment culture of reducing phenolic bacteria

[0051] Use an inoculation loop to pipette the depressor bacteria preserved in the 3-ring solid medium into a conical flask containing 100mL sterilized MPYE liquid medium, mix well, seal the mouth of the conical flask with a sealing film, and place it in a shaker. In the bed, the temperature is 30°C, the rotation speed is 200r / min, and the cultivation is carried out for 24 hours to obtain the enriched culture solution of reducing phenolic bacteria.

[0052] 3. Preparation of phenolic bacteria concentrate

[0053] Centrifuge the depletion bacteria enriched culture solution in a high-speed centrifuge at a speed of 8000r / min for 5 minutes, discard the supernatant, collect th...

Embodiment 3

[0057] 1. Preparation of immobilization solution

[0058] 1% agar + 3% carrageenan: Weigh 1g agar and 3g carrageenan and mix, dissolve in distilled water, make up to 100mL, sterilize at 121°C for 30min, cool to 40-45°C after sterilization.

[0059] 2. Enrichment culture of reducing phenolic bacteria

[0060] Use an inoculation loop to pipette the depressor bacteria preserved in the 3-ring solid medium into a conical flask containing 100mL sterilized MPYE liquid medium, mix well, seal the mouth of the conical flask with a sealing film, and place it in a shaker. In the bed, the temperature is 30°C, the rotation speed is 200r / min, and the cultivation is carried out for 24 hours to obtain the enriched culture solution of reducing phenolic bacteria.

[0061] 3. Preparation of phenolic bacteria concentrate

[0062] Centrifuge the depletion bacteria enriched culture solution in a high-speed centrifuge at a speed of 8000r / min for 5 minutes, discard the supernatant, collect the bacte...

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Abstract

The invention provides phenol-degrading bacterium immobilization spherical granules and a preparation method and application thereof. A method that carrageenin and agar which are high in mechanical property are mixed in a certain proportion, is adopted, so that prepared mixed microspheres as a phenol-degrading bacterium carrier are applied to treatment of phenol-containing waste water. The phenol-degrading bacterium mixed immobilization spherical granules formed by the method have high mechanical properties, small bore diameter and large specific surface area, can overcome the defects of bacterium revelation, and besides, can be favorable for increment of the mass transfer rate of the system, so that the sufficient bacterial adhesive rate is guaranteed, and the phenol-degrading propertiesand the cyclic utilization efficiency of the phenol-degrading bacterium immobilization spherical granules are improved.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and relates to an immobilized spherical particle of reducing phenol bacteria, a preparation method and application thereof. Background technique [0002] Phenol-containing wastewater mainly refers to phenolic organic pollutant wastewater produced in coking, oil refining, papermaking, plastics, ceramics, textile and other industries. As one of industrial wastewater, it has a wide range of sources and has a huge impact on environmental pollution. Phenol and its derivatives belong to aromatic (Aromatic) compounds, which are protoplasmic poisons, have toxic effects on organisms, and are difficult to be degraded. Phenolic substances have been listed as one of the 129 priority pollutants blacklisted by the US Environmental Protection Agency. Phenol-containing wastewater has been listed as one of the harmful wastewater that needs to be addressed in my country's water pollution control...

Claims

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Application Information

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IPC IPC(8): C12N11/10C02F3/34C02F101/34
CPCC02F3/34C02F2101/345C12N11/10
Inventor 王雪青房玉婷张安龙陈朵罗清王哲毅
Owner SHAANXI UNIV OF SCI & TECH
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