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Application of APOO autoantibody detection reagent in preparation of lung cancer screening kit

An autoantibody and kit technology, applied in biological tests, measuring devices, material testing products, etc., can solve the problems of no lung cancer existing technology, no APOO protein, etc., and achieve the effect of effective screening and good application prospects.

Active Publication Date: 2020-02-11
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no related reports on APOO protein autoantibodies, and there is no prior art related to lung cancer.

Method used

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  • Application of APOO autoantibody detection reagent in preparation of lung cancer screening kit
  • Application of APOO autoantibody detection reagent in preparation of lung cancer screening kit
  • Application of APOO autoantibody detection reagent in preparation of lung cancer screening kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Relationship between APOO Autoantibodies in Plasma and Lung Cancer

[0027] 1. Clinical data

[0028] 30 lung cancer patients and 29 healthy controls were selected. The basic information is as follows:

[0029] Basic Information lung cancer patients healthy control number of people 30 29 age 49.5±5.7 42.0±8.9 male ratio 20(66.7％) 13(44.8％)

[0030] 2. Detection principle

[0031] HuProt TM APOO protein is immobilized on the human protein custom chip (the APOO protein used is a full-length protein, and the protein sequence number is Ensembl: ENSP00000488524). After incubation with serum, APOO autoantibodies (mainly including IgG and IgM antibodies, and some Other types of antibodies) will bind to it, wash to remove unbound antibodies and other proteins, and then detect with anti-human IgM fluorescently labeled secondary antibody (cy5-labeled, red) and anti-human IgG fluorescent secondary antibody (cy3-labeled, green) ,...

Embodiment 2

[0048] Embodiment 2 The composition of detection kit of the present invention and its using method

[0049] 1. Kit composition

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Abstract

The invention relates to the field of in-vitro diagnostic reagents, in particular to application of an APOO autoantibody detection reagent in preparation of a lung cancer screening kit. It is found for the first time that the autoantibody level of APOO protein in serum of a lung cancer patient is significantly lower than that in a healthy one. The reagent for detecting the APOO protein autoantibody is used for preparing the lung cancer screening kit, and effective screening of lung cancer can be achieved.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic reagents, in particular to the use of APOO autoantibody detection reagents in the preparation of lung cancer screening kits. Background technique [0002] Lung cancer is one of the most common malignant tumors in the world. Its morbidity and mortality are increasing year by year. Currently, the incidence rate ranks first in the world, which seriously threatens human health and life. [0003] Lung cancer is a disease that is good at concealment. It often shows clinical symptoms when the disease develops to an advanced stage. 70-80% of lung cancer patients are already in the middle or late stage when they are diagnosed with lung cancer symptoms. The best cure time, five-year survival rate is low. For patients with early lung cancer, timely treatment can greatly improve the 5-year survival rate and quality of life of the patients. Therefore, early diagnosis and effective screening of lung cancer ...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/68
CPCG01N33/57423G01N33/57488G01N33/6854G01N2333/775
Inventor 李为民张立
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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