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Sweet potato stem tip detoxification and culture method

A sweet potato stem tip and sweet potato technology, applied in the field of detoxification and breeding of sweet potato stem apex, can solve the problems of difficult operation and low detoxification efficiency, achieve rapid growth of seedlings, improve callus ability, and improve detoxification efficiency Effect

Inactive Publication Date: 2020-02-21
TIANJIN FENGHUA YULONG AGRI DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The more common detoxification method is to combine shoot tip culture or young shoot grafting with heat treatment, low temperature treatment and chemical treatment. However, these methods have disadvantages such as difficult operation and low detoxification efficiency.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A method for detoxification and breeding of sweet potato shoot tips, comprising the following specific steps:

[0040] (1) The extraction of sweet potato stem tips

[0041] Among fresh sweet potatoes, select tubers without mildew and lesions, soak them in ribavirin solution, and put them in a 34°C incubator for cultivation. When the shoots grow to 3.5 cm, cut off the 1 mm-long shoot apex meristem, and use Rinse with tap water for 10 minutes before use;

[0042] (2) Disinfection and basic cultivation of shoot tips

[0043] Soak the shoot apical meristem in 5% hydrogen peroxide for 2 minutes, sterilize it under ultraviolet light for 2 minutes, rinse it with sterile water for 4 times, inoculate the shoot apical meristem on the pre-culture medium and cultivate it for 1 day;

[0044] (3) Virus-free treatment of stem tips

[0045] Rinse the shoot apical meristem after the pre-cultivation in step (2) with sterile water, put it into the anti-pollution medium at room temperat...

Embodiment 2

[0055] A method for detoxification and breeding of sweet potato shoot tips, comprising the following specific steps:

[0056] (1) The extraction of sweet potato stem tips

[0057] Among fresh sweet potatoes, select tubers without mildew and lesions, soak them in ribavirin solution and put them in a 34°C incubator for cultivation. When the buds grow to 5cm, cut off the 2mm-long shoot apex meristem, and wash them with tap water. Rinse for 20 minutes before use;

[0058] (2) Disinfection and basic cultivation of shoot tips

[0059] The shoot apical meristem is soaked in 15% hydrogen peroxide for 4 minutes, sterilized under an ultraviolet lamp for 3 minutes, and then rinsed with sterile water for 5 times, and the shoot apical meristem is inoculated on the pre-medium and cultivated for 2 days;

[0060] (3) Virus-free treatment of stem tips

[0061] Rinse the shoot apical meristem after the pre-cultivation in step (2) with sterile water, put it into anti-pollution medium at room ...

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Abstract

The invention relates to a sweet potato stem tip detoxification and culture method, which includes the following specific steps: firstly, culturing sweet potato blocks without the mildew disease untilsprouting, then cutting stem tip meristems, sterilizing with hydrogen peroxide, placing the stem tip meristems into a pre-culture medium for culturing, flushing the stem tip meristems, placing the stem tip meristems into an anti-pollution culture medium for culturing to obtain virus-free seedlings, inoculating the virus-free seedlings into a proliferation culture medium for culturing, and screening out non-toxic plants for greenhouse culturing. According to the method, indole-3-acetic acid is added, so that the ability of the stem tips to form calluses is improved, and seedlings grow fast; and at the same time, the stem tip meristems are put into the anti-pollution culture medium for culturing, so that the problems of low virus-free efficiency and high pollution rate during cutting off and rapid propagation in sweet potato virus-free tissue culture can be avoided, and the virus-free efficiency is effectively improved.

Description

technical field [0001] The invention relates to the technical field of detoxification of sweet potato shoot tips, in particular to a method for detoxification and breeding of sweet potato shoot tips. Background technique [0002] Sweet potato, also known as sweet potato, sweet potato, etc., is a plant of the genus Dioscorea in the family Convolvulaceae. Sweet potato is widely planted and is an important global food crop, as well as an important raw material for light industry and feed. Sweet potato is rich in various nutrients such as soluble sugar, vitamin C and dietary fiber, and has health care functions such as lowering cholesterol and preventing cardiovascular and cerebrovascular diseases. Therefore, it is listed as the first of the 13 best vegetables by the World Health Organization. [0003] Sweet potatoes are mainly planted through cuttings. Various viruses can be transmitted by insects and juices, and the combined infection rate is very high. Sweet potato plume mo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 王立国
Owner TIANJIN FENGHUA YULONG AGRI DEV CO LTD
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