Virus-free solid culture medium for stem apexes of sweet potatoes and method for preparing virus-free solid culture medium

A technology of solid culture medium and sweet potato shoot tips, applied in the directions of botanical equipment and methods, horticultural methods, applications, etc., can solve the problems of inconvenient adjustment of pH value, increase production cost, prolong cultivation period, etc., and improve the emergence rate of shoot tips. , the effect of saving production costs and shortening the cultivation cycle

Inactive Publication Date: 2017-09-12
TIANJIN FENGHUA YULONG AGRI DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Virus-free tissue culture of sweet potato shoot tip refers to the process of inducing callus and regenerating plants from the non-toxic or virus-free shoot tip of sweet potato. This process is mainly carried out on solid medium, but the traditional solid medium It is inconvenient to adjust the pH value. When the traditional solid medium is used, the emergence rate is low. Due to the poor ability of the shoot tip to form callus, the growth of the seedlings is slow, which greatly prolongs the culture period and increases the production cost.

Method used

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  • Virus-free solid culture medium for stem apexes of sweet potatoes and method for preparing virus-free solid culture medium
  • Virus-free solid culture medium for stem apexes of sweet potatoes and method for preparing virus-free solid culture medium
  • Virus-free solid culture medium for stem apexes of sweet potatoes and method for preparing virus-free solid culture medium

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Embodiment 1

[0020] A kind of solid culture medium for detoxification of sweet potato shoot tip is made up of the following raw materials in parts by weight:

[0021]

[0022]

[0023] Preparation method: Taking the preparation of the solid medium for detoxification of sweet potato shoot apex as an example, the preparation method of a kind of solid medium for detoxification of sweet potato shoot apex provided by the present embodiment is described as follows:

[0024] (1) Take 10 parts of potassium nitrate, 25 parts of ammonium nitrate, 5 parts of magnesium sulfate, 3 parts of calcium chloride, 0.01 parts of potassium iodide, 0.02 parts of copper sulfate, 0.4 parts of disodium edetate, 0.02 parts of Cobalt chloride, 0.2 part of ferrous sulfate, and they are mixed and prepared into basal medium;

[0025] (2) Add 3 parts of glycine, 0.3 parts of thiamine hydrochloride, 50 parts of inosose, 80 parts of sucrose, 1 part of bromocresol purple and 0.2 part of indole-3 to the basal medium in...

Embodiment 2

[0029] A kind of solid culture medium for detoxification of sweet potato shoot tip is made up of the following raw materials in parts by weight:

[0030]

[0031] Preparation method: Taking the preparation of the solid medium for detoxification of sweet potato shoot apex as an example, the preparation method of a kind of solid medium for detoxification of sweet potato shoot apex provided by the present embodiment is described as follows:

[0032] (1) Weigh 15 parts of potassium nitrate, 30 parts of ammonium nitrate, 8 parts of magnesium sulfate, 5 parts of calcium chloride, 0.03 parts of potassium iodide, 0.04 parts of copper sulfate, 0.7 parts of disodium edetate, 0.02 parts of Cobalt chloride, 0.4 part of ferrous sulfate are mixed and prepared into basal medium;

[0033] (2) Add 6 parts of glycine, 0.5 part of thiamine hydrochloride, 65 parts of inosose, 100 parts of sucrose, 3 parts of bromocresol purple and 0.4 part of indole-3 to the basal medium in step (1) according ...

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Abstract

The invention relates to a solid medium for detoxification of sweet potato stem tips and a preparation method thereof, which is composed of the following raw materials in parts by weight: 100-200 parts of agar; 43-58 parts of mother liquor I; 0.65-1.19 parts of mother liquor II; 3 parts of glycine ‑6 parts; thiamine hydrochloride 0.3‑0.5 parts; inosose 50‑65 parts; sucrose 80‑100 parts; bromocresol purple 1‑3 parts; indole‑3‑acetic acid 0.2‑0.4 parts; ; The preparation method: first prepare the base medium; then make the complete medium; add agar to stir and mix, and prepare the solid medium after high-pressure steam sterilization. The present invention facilitates the adjustment of the pH value of the solid medium by adding bromocresol violet; by adding sucrose, the germination rate of the shoot tip is greatly improved; by adding indole-3-acetic acid, the ability of the shoot tip to form callus is improved , the seedlings grow quickly, thus greatly shortening the cultivation period and saving the production cost.

Description

technical field [0001] The invention relates to the technical field of sweet potato tissue culture, in particular to a solid medium for detoxification of sweet potato shoot tips and a preparation method thereof. Background technique [0002] Virus-free tissue culture of sweet potato shoot tip refers to the process of inducing callus and regenerating plants from the non-toxic or virus-free shoot tip of sweet potato. This process is mainly carried out on solid medium, but the traditional solid medium It is inconvenient to adjust the pH value. When the traditional solid medium is used, the emergence rate is low. Due to the poor ability of the shoot tip to form callus, the growth of the seedlings is slow, which greatly prolongs the culture period and increases the production cost. Contents of the invention [0003] The invention aims to solve the deficiencies of the prior art, and provides a solid medium for detoxification of sweet potato shoot tips and a preparation method th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 王立国王刚李燕李建军高翔张嘉
Owner TIANJIN FENGHUA YULONG AGRI DEV CO LTD
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