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Micro-droplet preparation chip for detecting intestinal microorganisms

A technology for gut microbes and microdroplets, applied in the field of microfluidics, can solve the problems of unfavorable digital PCR detection, change of droplet size, and difficulty in regulation.

Pending Publication Date: 2020-02-21
THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although, such a problem can be solved by adjusting the flow rate of the continuous phase during the secondary wrapping so that the velocity of the generated droplets just matches the droplet spacing of the previous stage, so as to avoid the occurrence of single-wrapped droplets during the secondary wrapping, but in this way It is obviously difficult to adjust; more importantly, as mentioned earlier, changes in the flow rate of the continuous phase will lead to changes in the size of the droplets produced, such as reducing the flow rate of the continuous phase will lead to an increase in droplet size, and this has a significant impact on the number of PCR testing is unfavorable

Method used

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  • Micro-droplet preparation chip for detecting intestinal microorganisms
  • Micro-droplet preparation chip for detecting intestinal microorganisms
  • Micro-droplet preparation chip for detecting intestinal microorganisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] like Figure 4-5 As shown, a micro-droplet preparation chip for the detection of intestinal microorganisms is provided, and the micro-droplet preparation chip is used to prepare double-wrapped droplets, including a cover sheet layer and a microchannel layer; the upper layer of the microchannel layer The side surface is formed with a non-through microchannel structure, the cover sheet layer covers the upper surface of the microchannel layer, and one side thereof is provided with four through holes in fluid communication with the microchannel layer; The channel structure includes an inner core fluid channel 1, a first continuous phase channel 2, a single-wrapped droplet channel 3, a second continuous phase channel 5, and a double-wrapped droplet channel 6; the inner core fluid channel 1 and the first continuous phase channel 2 cross intersections, the downstream of the intersection is fluidly connected to the single-encapsulated droplet channel 3 through a diameter-reduci...

Embodiment 2

[0055] like Figure 4 , 6 As shown, a micro-droplet preparation chip for the detection of intestinal microorganisms is provided, which has a main structure similar to that of the micro-droplet preparation chip described in Example 1, the difference is that each of the single-package droplet channels 3 The regulating channels 7 on the side are all composed of four sub-channels 71 arranged side by side, and the distance between any two adjacent dry sub-channels 71 is 0.5-3 times the width of the sub-channels 71 themselves.

Embodiment 3

[0057] like Figure 4 , 7 As shown in -8, a micro-droplet preparation chip for the detection of intestinal microorganisms is provided. What is different from Examples 1 and 2 is that the adjustment channel 7 located on each side of the single-package droplet channel 3 includes The collection channel 72 that expands in the liquid flow direction and the drainage grid 73 arranged at the junction of the collection channel and the droplet channel, the connection line formed by the inner edges of the plurality of drainage grids 73 and the single-wrapped droplet channel The extension line of the side wall of 3 coincides or is located outside the extension line, so that the single-package droplet will not be hindered during the flow process in the single-package droplet channel 3; the drainage grid 73 is composed of several microcolumns 731 Composition, the gap 732 between two adjacent micropillars 731 forms the flow path of the first continuous phase between the single-wrapped dropl...

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Abstract

The invention relates to a droplet preparation chip for detecting intestinal microorganisms, which can accurately control and adjust the size of multiple coated droplets by arranging an adjusting channel; according to the change of droplet preparation requirements, multiple coated droplets with different sizes can be obtained by adjusting the change of channel power parameters; meanwhile, the adjustment is not limited to the overall size of the multiple coated droplets, and the core size, the coating layer thickness and the like of the multiple coated droplets can be independently adjusted; the droplet preparation chip allows preparation of homogenized double emulsion droplets of different specifications on the same chip, has universality within a certain droplet size range, and can be used as an independent droplet preparation unit to be combined with droplet use units of various specifications, so that the detection cost is greatly reduced.

Description

technical field [0001] The invention relates to the field of microfluidic technology, in particular to a micro-droplet preparation chip for detecting intestinal microorganisms. Background technique [0002] Intestinal microbes refer to the general term of microbial communities living in the human intestinal tract, including bacteria, archaea, and single-celled eukaryotes, which together with the intestinal environment constitute a huge and complex ecosystem. The human gut is a nutrient-rich microenvironment, carrying up to 100 trillion bacteria, and the number of genes in human gut microbes is 5 million, which is 150 times the number of human genes. Gut microbes are related to metabolic diseases, cardiovascular diseases, digestive system diseases, cancer, immune system diseases and central nervous system diseases. The research on their structure, function and pathogenic mechanism is beneficial to the treatment of diseases. and the development of new therapeutic modalities. ...

Claims

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Application Information

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IPC IPC(8): C12M1/00
CPCB01L3/5027
Inventor 徐迹曾晶晶吴明徐敏刘文兰徐勇黄建林
Owner THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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