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Use of extract of artocarpus heterophyllu white core for regulating gene expression and improving menopausal syndrome

A jackfruit and extract technology, applied in the field of plant extracts, can solve problems such as side effects

Inactive Publication Date: 2020-02-28
TCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term administration or excessive use of sex hormones may cause breast cancer and have side effects

Method used

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  • Use of extract of artocarpus heterophyllu white core for regulating gene expression and improving menopausal syndrome
  • Use of extract of artocarpus heterophyllu white core for regulating gene expression and improving menopausal syndrome
  • Use of extract of artocarpus heterophyllu white core for regulating gene expression and improving menopausal syndrome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preparation of the extract of embodiment 1. jackfruit white core

[0032] First, prepare the white core of jackfruit and clean it for subsequent extraction. Next, take the washed jackfruit white core and homogenize it, and then extract the homogenate with water (2-10:1-5 liquid-solid ratio) at 50-100° C. for 0.5-3 hours. Afterwards, cool to room temperature and filter through a 400-mesh filter, and then concentrate the filtered product under reduced pressure at 45-70° C. to obtain the extract of jackfruit white core.

Embodiment 2

[0033] Example 2. Evaluation of the effectiveness of the extract of jackfruit white core in improving osteoporosis

[0034] 2.1 Inhibition of osteoclast differentiation test

[0035] First, peripheral blood (PB) was provided by a healthy donor, and then 20 mL of peripheral blood was diluted with 15 mL of PBS. Next, 15 mL of Ficoll (GE Healthcare) was added to a centrifuge tube having a volume of 50 mL, and then 35 mL of diluted peripheral blood was added on the Ficoll layer, and centrifuged at 400 g for 40 minutes. Afterwards, peripheral blood mononuclear cells (PBMCs) were taken out and washed twice with PBS.

[0036] Then, the peripheral blood mononuclear cells were divided into two groups, including a control group and an experimental group. Add 10% fetal bovine serum (FBS) (Gibco), 1X penicillin (penicillin) / streptomycin (streptomycin) (Gibco), 40ng / mL human nuclear factor κ-B ligand receptor activator (receptor activator α-MEM (Gibco , 12000-022) were used as the oste...

Embodiment 3

[0048] Example 3. Evaluation of the efficacy of the extract of jackfruit white core on cardiovascular health care

[0049] In this example, the applicant explores whether the extract of jackfruit white core can achieve the effect of cardiovascular health by regulating the expression of genes related to HDL production and cholesterol metabolism.

[0050] First, HepG2 cells were cultured in 6-well culture plates in DMEM medium (Gibco, 12100-038) supplemented with FBS (Gibco, 10437-028), penicillin / streptomycin (Gibco, 15140122), and the cell concentration was 1× 10 5 cells / well, followed by incubation at 37°C for 24 hours, and removal of medium.

[0051] Afterwards, the cultured cells were divided into two groups, including a control group and an experimental group. The extract of jackfruit white core obtained in Example 1 was diluted with a culture medium to have a concentration of 0.25 mg / ml, and then added to the cells of the experimental group, and the culture medium was a...

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PUM

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Abstract

The invention relates to the field of plant extracts, particularly to use of an extract of an artocarpus heterophyllu white core for regulating gene expression and improving a menopausal syndrome. Theextract of an artocarpus heterophyllu white core can be used for regulating expression of CFOS gene, CSF1R gene, RANK gene, SYK gene, TRAP gene, APA1 gene, ABCA1 gene, IL-6 gene, VCAM1 gene, CASP8 gene, DDC gene and ASMTL protein gene, and improving A menopausal syndrome.

Description

technical field [0001] The invention relates to the field of plant extracts, in particular to the use of a jackfruit (Artocarpusheterophyllus) white core extract for regulating gene expression and improving menopausal syndrome. Background technique [0002] Menopause is associated with a rapid decrease in circulating sexual hormones in a woman, and refers to the transition period in which a woman's ovarian function gradually declines, eventually leading to the cessation of menstruation, while Postmenopause refers to the period after a woman has completely lost ovarian function. Menopausal syndrome (menopausal syndrome) usually occurs in menopausal or postmenopausal women, and the symptoms of menopausal syndrome mainly include: hot flushes (hot flushes), osteoporosis (osteoporosis), cardiovascular disease (cardiovascular disease), insomnia ( insomnia), skin aging, anxiety and depression. [0003] At present, hormone replacement therapy (HRT) is mostly used in the treatment ...

Claims

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Application Information

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IPC IPC(8): A61K36/60A61P15/12A61P19/10A61P9/00A61P25/20A23L33/00A61K131/00
CPCA23V2002/00A61K36/60A23L33/00A61P9/00A61P15/12A61P19/10A61P25/20A23V2200/306A23V2200/326A23V2200/322A23V2200/30
Inventor 林咏翔
Owner TCI CO LTD
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