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A kind of endophytic Aspergillus strain of Ophiopogon japonicus and its application

A technology of producing Aspergillus and Aspergillus, which is applied in the field of microorganisms, can solve the problems of Hubei Ophiopogon japonicus polysaccharide market restrictions, etc., and achieve the effects of avoiding unfavorable factors, great application value, and avoiding pollution

Active Publication Date: 2021-03-02
HUBEI UNIV OF ARTS & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The main purpose of the present invention is to propose a strain of the endophytic Aspergillus genus of Ophiopogon japonicus and its application, aiming to solve the problem that various unfavorable factors in the planting and production of Ophiopogon japonicus in Hubei lead to the restriction of the market release of Ophiopogon japonicus polysaccharides in Hubei

Method used

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  • A kind of endophytic Aspergillus strain of Ophiopogon japonicus and its application
  • A kind of endophytic Aspergillus strain of Ophiopogon japonicus and its application
  • A kind of endophytic Aspergillus strain of Ophiopogon japonicus and its application

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Experimental program
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Effect test

preparation example Construction

[0045] The preparation method of above-mentioned Hubei Ophiopogon japonicus polysaccharide comprises the following steps:

[0046] Step A10, take the above-mentioned Aspergillus sp. bacterium EF029, under aseptic conditions, pick a small amount of hyphae with an inoculation needle, insert it into a sterilized PDA medium test tube, and activate and cultivate it at 28° C. for 72 hours;

[0047] Step A20, taking the activated cultured strain, transferring it into a sterilized liquid potato seed medium under aseptic conditions, and vibrating at 180 rpm at 28°C for 72 hours to obtain seeds;

[0048] Step A30, put the prepared liquid fermentation medium into 250mL Erlenmeyer flasks, each bottle is about 100mL, sterilize and cool for later use; under sterile conditions, insert the seeds according to the inoculum size of 10%, at 28°C 180rpm shaking culture for 7 days;

[0049] Step A40, after the fermentation is completed, the fermented solid-liquid mixture is quickly frozen into a s...

specific Embodiment

[0056] Take the above-mentioned Aspergillus sp. (Aspergillus sp.) bacteria EF029, under aseptic conditions, pick a small amount of hyphae with an inoculation needle, insert it into a sterilized PDA medium test tube, and activate it at 28 ° C for 72 hours; take the activated culture Under aseptic conditions, transfer the strains after the strain into the sterilized liquid potato seed culture medium, shake and cultivate them at 180rpm at 28°C for 72 hours to obtain seeds; put the prepared liquid fermentation medium into 250ml pots respectively. In a triangular flask, each bottle is about 100ml, sterilized, and cooled for later use; under sterile conditions, insert the seeds according to the inoculum amount of 10%, and vibrate at 180rpm at 28°C for 7 days; after fermentation, put the fermented solid-liquid mixture in After being quickly frozen into a solid state in an ultra-low temperature refrigerator, a small freeze dryer is used for low-temperature freeze-drying to obtain a dri...

Embodiment 1

[0058] Embodiment 1 produces the screening of the bacterial strain of Ophiopogon japonicus polysaccharide

[0059] (1) Use a large amount of tap water to fully wash the soil on the surface of fresh Hubei Ophiopogon japonicus tubers, then put them into a clean beaker, add deionized water, and place them in an ultrasonic cleaner for repeated cleaning until the water after cleaning becomes extremely clear; After the bacteria filter paper is dipped to dry the water on the surface of the tuber root of Hubei Ophiopogon japonicus, it is processed according to the following steps: first soak the tuber tuber with 75% (V / V) ethanol for 2 minutes, rinse it with sterile water for 3 times; % sodium hypochlorite solution for 2 minutes, rinse with a large amount of sterile water for 4 times, and then use sterile filter paper to dry the water;

[0060] (2) Take the above surface-sterilized Ophiopogon japonicus root tuber sample, cut off the browned part at both ends of the tuber under aseptic...

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Abstract

The invention discloses an endophytic Aspergillus strain of Ophiopogon japonicus Hubei and its application. The endophytic Aspergillus strain of Ophiopogon japonicus Hubei is Aspergillus sp. EF029, the preservation number is CCTCC No: M2018781, and the preservation time is for November 12, 2018. The endophytic Aspergillus strain of Ophiopogon japonicus provided by the present invention can be applied to the preparation of Ophiopogon japonicus polysaccharides, thereby avoiding environmental pollution caused by chemical synthesis in the existing method for preparing Ophiopogon japonicus hubei polysaccharides, and more avoiding the cultivation of Ophiopogon japonicus medicinal materials Various unfavorable factors in the production are important microorganisms to search for new resources of Ophiopogon japonicus polysaccharides in Hubei, and have great application value.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to an endophytic Aspergillus strain of Ophiopogon japonicus from Hubei and its application. Background technique [0002] Hubei Ophiopogon japonicus polysaccharide is one of the main active ingredients of Hubei Ophiopogon japonicus Liriopespicata var. It has pharmacological effects on thrombosis, hypoxia resistance, anti-aging, hypoglycemia, etc. It has a positive impact on improving the body's immune system and enhancing gastrointestinal motility. The latest research shows that it also has anti-tumor effects. [0003] The traditional Hubei Ophiopogon japonicus polysaccharide production is mainly obtained by directly separating the tuber root extract of Ophiopogon japonicus. However, in the actual planting production of Ophiopogon japonicus in Hubei, there are factors such as "relatively small planting area due to continuous cropping obstacles", "greatly affected by climatic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P19/04C12R1/66
CPCC12N1/14C12P19/04C12N1/145C12R2001/66
Inventor 余海忠王颖张得祥田文清于博王海燕黄升谋阎华孙永林李云捷
Owner HUBEI UNIV OF ARTS & SCI
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