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Endophytic aspergillus strain of radix ophiopogonis from Xiangyang in Hubei province and application thereof

A technology of Aspergillus and Aspergillus genus, applied in the field of microorganisms, can solve the problems of Hubei Ophiopogon japonicus polysaccharide market restrictions, etc., and achieve the effects of avoiding unfavorable factors, avoiding pollution, and having great application value

Active Publication Date: 2020-03-24
HUBEI UNIV OF ARTS & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The main purpose of the present invention is to propose an endophytic Aspergillus strain of Xiangmaidong and its application, aiming to solve the problem that the marketing of Hubei Ophiopogon japonicus polysaccharides is limited due to various unfavorable factors in the planting and production of Xiangmaidong medicinal materials

Method used

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  • Endophytic aspergillus strain of radix ophiopogonis from Xiangyang in Hubei province and application thereof
  • Endophytic aspergillus strain of radix ophiopogonis from Xiangyang in Hubei province and application thereof
  • Endophytic aspergillus strain of radix ophiopogonis from Xiangyang in Hubei province and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0045] The preparation method of above-mentioned Hubei Ophiopogon japonicus polysaccharide comprises the following steps:

[0046] Step A10, take the above-mentioned Aspergillus sp. bacterium EF063, under aseptic conditions, pick a small amount of hyphae with an inoculation needle, insert it into a sterilized PDA medium test tube, and activate and cultivate it at 28° C. for 72 hours;

[0047] Step A20, taking the activated cultured strain, transferring it into a sterilized liquid potato seed medium under aseptic conditions, and vibrating at 180 rpm at 28°C for 72 hours to obtain seeds;

[0048] Step A30, put the prepared liquid fermentation medium into 250mL Erlenmeyer flasks, each bottle is about 100mL, sterilize and cool for later use; under sterile conditions, insert the seeds according to the inoculum size of 10%, at 28°C 180rpm shaking culture for 7 days;

[0049] Step A40, after the fermentation is completed, the fermented solid-liquid mixture is quickly frozen into a s...

specific Embodiment

[0056] Take the above-mentioned Aspergillus sp. (Aspergillus sp.) bacteria EF063, under aseptic conditions, pick a small amount of hyphae with an inoculation needle, insert it into a sterilized PDA medium test tube, and activate it at 28°C for 72 hours; take the activation culture Under aseptic conditions, transfer the strains after the strain into the sterilized liquid potato seed culture medium, shake and cultivate them at 180rpm at 28°C for 72 hours to obtain seeds; put the prepared liquid fermentation medium into 250ml pots respectively. In a triangular flask, each bottle is about 100ml, sterilized, and cooled for later use; under sterile conditions, insert the seeds according to the inoculum amount of 10%, and vibrate at 180rpm at 28°C for 7 days; after fermentation, put the fermented solid-liquid mixture in After being quickly frozen into a solid state in an ultra-low temperature refrigerator, a small freeze dryer is used for low-temperature freeze-drying to obtain a drie...

Embodiment 1

[0058] Embodiment 1 produces the screening of the bacterial strain of Ophiopogon japonicus polysaccharide

[0059] (1) Use a large amount of tap water to fully wash the soil on the surface of Ophiopogon japonicus root tubers in Hubei, then put them into a clean beaker, add deionized water, and place them in an ultrasonic cleaner for repeated cleaning until the water after cleaning becomes extremely clear; After the filter paper is dipped to dry the water on the surface of the tuber root of Ophiopogon japonicus in Hubei Province, it is processed according to the following steps: first soak the tuber tuber with 75% (V / V) ethanol for 2 minutes, rinse it with sterile water for 3 times; then use 4-6% available chlorine Soak in sodium hypochlorite solution for 2 minutes, then rinse continuously with a large amount of sterile water for 4 times, and then dip dry with sterile filter paper;

[0060] (2) Take the above surface-sterilized Ophiopogon japonicus root tuber sample, cut off th...

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Abstract

The invention discloses an endophytic aspergillus strain of radix ophiopogonis from Xiangyang in Hubei province and application thereof. The endophytic aspergillus strain is aspergillus strain EF063 and has the preservation number of CCTCC No: M2018784; and the preservation time is November 12th, 2018. The endophytic aspergillus strain of radix ophiopogonis from Xiangyang can be applied to preparation of radix ophiopogonis polysaccharide in Hubei province, thereby avoiding environmental pollution caused by chemical synthesis in the existing method for preparing the Hubei radix ophiopogonis polysaccharide. Various adverse factors in planting production of Xiangyang radix ophiopogonis medicinal materials can be avoided. The endophytic aspergillus strain is an important microorganism for searching for new resources of the Hubei radix ophiopogonis polysaccharide and has the high application value.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to an endophytic Aspergillus strain of Xiangmaidong and its application. Background technique [0002] Hubei Ophiopogon japonicus polysaccharide is one of the main active ingredients of Hubei Ophiopogon japonicus Liriope spicata var. Anti-myocardial ischemia, anti-thrombosis, anti-hypoxia, anti-aging, hypoglycemic and other pharmacological effects, it has a positive impact on improving the body's immune system and enhancing gastrointestinal motility. Has anti-tumor effect. [0003] The traditional production of Ophiopogon japonicus polysaccharides in Hubei is mainly obtained by directly separating the root extract of Ophiopogon japonicus. However, in the actual planting and production of Xiangmaidong, there are factors such as "relatively small planting area due to continuous cropping obstacles", "greatly affected by climatic conditions", "long production cycle of root tube...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P19/04C12R1/66
CPCC12P19/04C12N1/145C12R2001/66
Inventor 余海忠郑炜炜张得祥田文清于博王海燕黄升谋阎华孙永林李云捷
Owner HUBEI UNIV OF ARTS & SCI
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