Gene sequencing chip and packaging method thereof

Abstract

The invention provides a gene sequencing chip and a packaging method thereof. The chip comprises a protecting frame, an aminated substrate and a cover plate, wherein the aminated substrate is arrangedin the protecting frame and the cover plate stacks on the substrate. The method is characterized in that the contact areas of the protecting frame, the substrate and the cover plate are adhered through the curing reaction of coated silica gel to form a sealed structure, wherein the silica gel comprises one or the combination of dealcoholized silica gel, deaminated silica gel and deamidized silicagel, and the silica gel and the curing reaction byproduct of the silica gel do not have reaction with amino. The method has the advantages that the silica gel series glue which does not have reactionwith the amino is used to perform adhering and sealing, the problem that the glue pollutes the surface of the chip during the packaging of the aminated chip is solved effectively, complete sequencingdata is guaranteed, and sequencing quality is increased.

Description

technical field [0001] The invention relates to the technical field of gene sequencing, in particular to a method for encapsulating a gene sequencing chip and a gene sequencing chip formed by applying the method. Background technique [0002] This section is intended to provide a background or context for implementations of the invention that are recited in the claims. The descriptions herein are not admitted to be prior art by inclusion in this section. [0003] The gene chip is an important carrier for gene sequencing, and its surface can be immobilized with DNA after certain modification. Then the DNA to be tested undergoes a series of biochemical reactions on the surface of the chip, and the sequence information of the DNA to be tested can be obtained through detection by related instruments. The chip whose surface is modified with amino groups is a kind of gene chip, and the purpose of immobilizing DNA is achieved by using the interaction between amino groups on the s...

AI Technical Summary

Problems solved by technology

However, since the sequencing chip is detected at the nanometer level, a little bit of glue diffusion and contamination on the macroscopic level will be very obvious on the microscopic level, which will have a great impact on the test results.

Therefore, at the nanoscale, it is a disadvantage that the components of the glue or the curing reaction product will react with the amino groups, because the amino groups on the chip will be consumed, which will deteriorate the sequencing quality, indirectly reduce the amount of sequencing data, and ultimately increase the sequencing efficiency. cost

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