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Bacillus licheniformis producing thermoprotease and its application

A technology of Bacillus licheniformis and protease, applied in the application, bacteria, microorganism and other directions, to achieve the effects of simple production process, increased crude protein and active peptide content, and remarkable effect

Active Publication Date: 2022-06-21
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are still few studies on the use of high-temperature protease-producing strains to directly solid-state ferment oil cakes to produce biological feedstuffs without substrate sterilization.

Method used

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  • Bacillus licheniformis producing thermoprotease and its application
  • Bacillus licheniformis producing thermoprotease and its application
  • Bacillus licheniformis producing thermoprotease and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] (1) Strain activation: Bacillus licheniformis YYC4 was inoculated into LB slant medium under sterile conditions, cultured at 60°C for 24h to recover the strain, then streaked on LB plate medium, cultured at 60°C for 24h, and then picked Single colony strains were respectively inoculated into shaker flasks (250mL) equipped with 100mL liquid medium, cultured at 160r / min at 60°C for 24h to obtain Bacillus YYC4 fermentation broth; Expanding the culture in the liquid medium to obtain the Bacillus licheniformis fermented seed liquid;

[0045] The formula of LB medium: tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, pH 7.4;

[0046] (2) Cake fermentation: select soybean meal, sunflower meal and rapeseed meal for oil cake; take soybean meal, sunflower meal or rapeseed meal respectively 20g, oil cake and water according to the mass volume ratio of 1g: 0.8mL Mix in proportion to obtain a mixed solution; then add the Bacillus licheniformis fermented seed solution prepa...

Embodiment 2

[0050] (1) bacterial classification activation: concrete steps are with the step (1) of embodiment 1;

[0051] (2) Fermentation of oil cake: select soybean meal, sunflower meal and rapeseed meal for oil cake; take soybean meal, sunflower meal or rapeseed meal respectively 20g, oil cake and water according to the mass volume ratio of 1g: 2mL Mix in proportion to obtain a mixed solution; then add the Bacillus licheniformis fermented seed solution prepared in step (1) into the mixed solution, wherein the consumption ratio of the Bacillus licheniformis fermented seed solution and the mixed solution is 10mL: 100g, at a humidity of 80%, Ferment for 72 hours at a temperature of 60° C. to obtain the active peptide biological feed.

[0052] Control group 2: the operation is the same as the step (2) of embodiment 2, the difference is that the fermented seed liquid of Bacillus licheniformis is not inoculated.

[0053] The contents of fermented cake and control crude protein and active p...

Embodiment 3

[0055] Application of Bacillus licheniformis YYC4 in the preparation of bioactive peptide feed by oil cake fermentation:

[0056] (1) bacterial classification activation: concrete steps are with the step (1) of embodiment 1;

[0057] (2) Fermentation of oil cake: select soybean meal, sunflower meal and rapeseed meal for oil cake; take 20g each of soybean meal, sunflower meal or rapeseed meal respectively, and the mass-volume ratio of oil cake and water is 1g: 1.4mL Mix in the ratio to obtain a mixed solution; then add the Bacillus licheniformis fermented seed solution prepared in step (1) into the mixed solution, wherein the consumption ratio of the Bacillus licheniformis fermented seed solution and the mixed solution is 6mL: 100g, at a humidity of 60% and ferment for 54 hours at a temperature of 55° C. to obtain the active peptide biological feed.

[0058] Control group 3: the operation is the same as step (2) of embodiment 3, the difference is that the fermented seed liquid...

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Abstract

The invention belongs to the field of microbial screening and fermentation engineering, and relates to a high-temperature protease-producing Bacillus licheniformis and its application; the invention firstly provides a high-temperature protease-producing Bacillus licheniformis (Bacillus licheniformis) YYC4, and the preservation number is CCTCC NO:M 2019599; then apply Bacillus licheniformis YYC4 to the fermentation of oil cake to prepare bioactive peptide feed, the steps are: add oil cake to water, stir and mix to obtain a mixed solution; Fermentation is carried out under temperature conditions, and active peptide biological feed is obtained after fermentation; the high-temperature protease-producing strain provided by the invention can grow well under the condition of 60°C, and the oil cake protein is directly prepared by high-temperature solid-state fermentation under non-sterilizing conditions The bioactive peptide feed has remarkable effect, extremely low cost and broad application prospect.

Description

technical field [0001] The invention belongs to the field of microbial screening and fermentation engineering, and in particular relates to a high-temperature protease-producing bacillus licheniformis and the use of the high-temperature fermentation cake of the strain to prepare polypeptide biological feed. Background technique [0002] Thermobacteria are a class of important microorganisms whose optimum growth temperature is greater than 50°C. They can produce enzymes and other active substances with special functions. They have attracted more and more attention in terms of survival mechanism and application. The application of high-temperature bacteria to the fermentation industry can effectively solve the problem of the mismatch between the optimum growth temperature (25-37°C) of mesophilic microorganisms and the high-temperature environment (50-65°C) of the fermentation process. In addition, the generation time of high-temperature bacteria is short, and the synthesis of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/12A23K10/18C12R1/10
CPCA23K10/12A23K10/18C12R2001/10C12N1/205
Inventor 代春华侯宜芝何荣海黄六容马海乐
Owner JIANGSU UNIV