Mouse cartilage cataplasia inducing fibrous ring, nucleus pulposus cataplasia model and application

Abstract

The invention discloses a mouse cartilage cataplasia inducing fibrous ring, a nucleus pulposus cataplasia model and an application. The tail skin of a mouse is split, and after the vertebra is exposed, windowing is performed on the vertebra to eliminate bone marrow in a bone-marrow cavity and damage the bone-marrow cavity in the metaphysis; a small magnet is placed in the bone-marrow cavity to produce pressure and block the nutrition supply of a cartilage endplate, and the skin is sutured; after edema disappears, a magnetic ring of certain length sleeves the skin at of the tail of the mouse; two sides of the magnetic ring are fixed by an adhesive tape; and a plurality of layers of adhesive tapes are wound to the tail of the mouse and below the tail end of a sleeve. According to the mouse cartilage cataplasia inducing fibrous ring, the nucleus pulposus cataplasia model and the application disclosed by the invention, a new method is used to manufacture the cartilage cataplasia model to simulate the a pathological process that human cartilage endplate inflammation and cartilage endplate cataplasia caused by various factors easily cause intervertebral disc nucleus pulposus cataplasia;the cartilage and the intervertebral disc are not processed, so that the mouse cartilage cataplasia inducing fibrous ring and the nucleus pulposus cataplasia model more conform to a human body pathological change process; besides, the effects that nutrition supply is comprehensively blocked, positive magnetic force pressurizing is performed, and the cell aging and cataplasia are accelerated by thepositive magnetic field are achieved, consumed time is short, and obvious intervertebral disc cataplasia can be produced within about two weeks.

Description

Technical field [0001] The invention belongs to the technical field of animal experimental models, and in particular relates to a rat cartilage degeneration-induced annulus fibrosus and nucleus pulposus degeneration model and application. Background technique [0002] Intervertebral disc degeneration (IVDD) is considered a global health threat and one of the most important causes of low back pain. Intervertebral disc degeneration is a disease caused by a variety of factors such as genetic susceptibility, environment, smoking, aging, biochemical and biomechanical factors, among which the reduction of intervertebral disc cell nutrition is considered to be the final common way of intervertebral disc degeneration. Many studies have shown that the endplate is very important for the nutrient supply of the intervertebral disc. Since there is no blood supply to the intervertebral disc under normal circumstances, most nutrients diffuse through the cartilage endplate to nourish the interve...

AI Technical Summary

Problems solved by technology

[0005] (1) Existing technology simulates cartilage endplate inflammation and cartilage endplate degeneration leading to intervertebral disc degeneration, weakened annulus fibrosus resistance, prone to disorder and fracture, accelerated apoptosis of nucleus pulposus cells, stem cells in cartilage endplate and fibrosis The reduction of migration and replenishment will easily lead to the outward protrusion of the nucleus pulposus tissue and the nearby fibrous ring, compressing the nerves, and causing low back pain

[0006] (2) At this stage, there is no clear modeling method for cartilage endplate inflammation model, and animal models have not yet been constructed, and the efficiency of rat intervertebral disc degeneration (IVDD) model by acupuncture and external fixator pressure is low

[0007] Difficulty in solving the above technical problems: the experiment is more difficult and requires a higher level of surgical skills for the experimenter. If the size of the magnet and the bone marrow cavity do not match, the vertebrae need to be trimmed to a certain extent; compared with the acupuncture model, it is more complicated. Moreover, compared with the vertical pressure model of the external fixator, the pressure generated by the magnet is relatively small; the tail vertebra of the rat has a certain diameter requirement, and if it is too thin, it cannot be operated; it is inconvenient to purchase a strong magnet with a suitable size and a magnetic ring

Images