A rat model of cartilage degeneration-induced degeneration of annulus fibrosus and nucleus pulposus and its application

Abstract

The invention discloses a rat cartilage degeneration-induced degeneration model of annulus fibrosus and nucleus pulposus and its application. The rat tail skin is incised, the vertebrae are exposed and the vertebrae are opened, and the marrow in the marrow cavity is removed and the metaphysis is removed. The bone marrow cavity was destroyed; a small magnet was placed in the bone marrow cavity to generate pressure and block the nutrient supply of the cartilage endplate, and the skin was sutured. After the edema subsided, a magnetic ring of a certain length was placed on the skin of the tail of the rat, and the two sides of the magnetic ring were fixed with tape. ; Wrap multiple layers of tape around the rat's tail below the end of the sleeve. The invention uses a new method to build a cartilage degeneration model, and simulates the pathological process of human cartilage endplate inflammation and cartilage endplate degeneration caused by various factors that are more likely to cause degeneration of the intervertebral disc nucleus pulposus. The invention does not treat cartilage and intervertebral disc, which is more in line with the pathological change process of the human body, and comprehensively blocks nutrition supply, positive magnetic pressure, and positive magnetic field to accelerate cell aging and degeneration. of intervertebral disc degeneration.

Description

technical field [0001] The invention belongs to the technical field of animal experimental models, and in particular relates to a rat cartilage degeneration-induced degeneration model of annulus fibrosus and nucleus pulposus and its application. Background technique [0002] Intervertebral disc degeneration (IVDD) is considered a global health threat and is one of the most important causes of low back pain. Intervertebral disc degeneration is a disease caused by a variety of factors including genetic predisposition, environment, smoking, aging, biochemical and biomechanical factors, among which the reduction of intervertebral disc cell nutrition is considered to be the final common pathway of intervertebral disc degeneration. Many studies have shown that the endplate is crucial for the nutrient supply of the intervertebral disc. Since there is no blood supply to the intervertebral disc under normal conditions, most nutrients diffuse through the cartilage endplate to nourish ...

AI Technical Summary

Problems solved by technology

[0005] (1) Existing technology simulates cartilage endplate inflammation and cartilage endplate degeneration leading to intervertebral disc degeneration, weakened annulus fibrosus resistance, prone to disorder and fracture, accelerated apoptosis of nucleus pulposus cells, stem cells in cartilage endplate and fibrosis The reduction of migration and replenishment will easily lead to the outward protrusion of the nucleus pulposus tissue and the nearby fibrous ring, compressing the nerves, and causing low back pain

[0006] (2) At this stage, there is no clear modeling method for cartilage endplate inflammation model, and animal models have not yet been constructed, and the efficiency of rat intervertebral disc degeneration (IVDD) model by acupuncture and external fixator pressure is low

[0007] Difficulty in solving the above technical problems: the experiment is more difficult and requires a higher level of surgical skills for the experimenter. If the size of the magnet and the bone marrow cavity do not match, the vertebrae need to be trimmed to a certain extent; compared with the acupuncture model, it is more complicated. Moreover, compared with the vertical pressure model of the external fixator, the pressure generated by the magnet is relatively small; the tail vertebra of the rat has a certain diameter requirement, and if it is too thin, it cannot be operated; it is inconvenient to purchase a strong magnet with a suitable size and a magnetic ring

Images