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Eurotium Cristatum zinc-rich strain and domestication and fermentation method

A technology of S. coronis and fermentation method, which is applied in the field of zinc-rich strains of S. coronis and its domestication and fermentation. It can solve the problems of large gastrointestinal side effects, stimulation, and low absorption rate, and meet the needs of industrial production. Effect

Inactive Publication Date: 2020-03-27
NORTHWEST UNIV(CN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In daily life, people mainly take in zinc in the form of inorganic state, but its use is limited due to the stimulation of zinc ions to the mucous membrane, the side effects of the gastrointestinal tract, and the low absorption rate.

Method used

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  • Eurotium Cristatum zinc-rich strain and domestication and fermentation method
  • Eurotium Cristatum zinc-rich strain and domestication and fermentation method
  • Eurotium Cristatum zinc-rich strain and domestication and fermentation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Screening of the Zinc-rich wild bacterial strain of Coronoidis coronoides

[0027] Weigh 10g of Fuzhuan tea with bright yellow closed cystic shell from the commercially available "Jingwei Fucha" in Xi'an, put it into a mortar and roughly crush it, then add 90mL of sterile water with 3-5 glass beads in the Erlenmeyer flask. Shake and mix at 150rpm for about 30min. Take 1mL of the spore mixture and make a 10-fold gradient dilution with sterile water, and select 10 -3 and 10 -4 Draw 0.1 mL of the two gradient spore suspensions and spread on the PDA solid medium containing 5 mg / L zinc element, and culture at 30°C for 60 hours. Pick larger colonies and bright yellow colonies and streak them on a PDA plate containing 5 mg / L zinc element to continue screening for 8-10 generations, culture on a PDA slant, and store at 4°C.

Embodiment 2

[0028] Mutagenesis, domestication and isolation and purification of the wild bacterial strain of embodiment 2

[0029] The bacterial lawn screened in Example 1 was eluted with sterile physiological saline, dispersed with glass beads, and the concentration was adjusted to 2×10 6 -2×10 8 spores / mL of spore suspension, draw a certain amount of spore suspension and place it in a petri dish (so that the liquid level does not exceed 3mm), irradiate with a 20W ultraviolet lamp tube for 25min at a distance of 30cm, and draw 0.1mL of bacterial suspension into the culture dish. Containing different concentrations (0; 5; 10; 15; 20 mg / L) of inorganic zinc in PDA solid plates and coated. Incubate at 30°C for 60h. Select several colonies with large colonies and bright colors on different concentrations of zinc to streak on the PDA solid plate containing 5mg / L inorganic zinc, and compare the colony size and colony appearance again. Select the largest colony and the brightest color as the ...

Embodiment 3

[0031] Example 3 Preservation of the Zinc-rich strain of Coronoidis coronoides

[0032] Pick the bacterial lawn of Zinc-rich S. coronis that was screened in Example 2 on a solid PDA slope, and cultivate it at 30°C for about 60 hours. Add 20 mL of sterile water and scrape the bacterial lawn with a bamboo stick. After mixing, the spore concentration is 2×10 6 ~2×10 8 cells / mL, and then add PDA liquid medium containing 50% glycerol at a volume ratio of 1:1, and mix well. Pre-freeze at -20°C first, and then transfer to -80°C for medium and long-term storage. The spore germination rate is above 90%.

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Abstract

The invention discloses a Eurotium Cristatum zinc-rich strain (Eurotium Cristatum XD-02) which is preserved in the China general microbiological culture Collection center on April 2, 2018, and the preservation number is CGMCC No. 15396. In addition, the invention further discloses a domestication and fermentation method of the strain. The Eurotium Cristatum zinc-rich strain is derived from Fuzhuantea, is ecological, safe and harmless, can tolerate inorganic zinc on an optimized culture medium, can efficiently convert the inorganic zinc into organic zinc, has the intracellular organic zinc content of 380 mg / kg, can be used as a production strain for efficiently converting the inorganic zinc into the organic zinc, and meets the industrial production requirements.

Description

technical field [0001] The invention belongs to the technical field of biochemical engineering, and in particular relates to a zinc-rich strain of Saccharomyces coronoides and a domestication and fermentation method. Background technique [0002] Zinc element is an essential nutrient element for human and animal body to maintain the life process, and it is an indispensable trace element, which specifically participates in the oxygen transport process in the blood. Zinc deficiency can cause symptoms such as "zinc deficiency anemia" in humans and animals. In daily life, people mainly take in zinc in the form of inorganic state, but its use is limited due to the stimulation of zinc ions to the mucous membrane, the side effects of the gastrointestinal tract, and the low absorption rate. [0003] Research on low-toxic and high-efficiency zinc supplements has always been the direction of development. Using biotransformation method to convert inorganic state zinc into organic sta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/02C12N13/00C12N1/36C12R1/645
CPCC12N1/02C12N1/14C12N1/36C12N13/00C12N1/145C12R2001/645
Inventor 范代娣惠俊峰段志广宋养斌
Owner NORTHWEST UNIV(CN)
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