[0107] 3) Test method
[0108] Use potted plants. See 1.1 for weed cultivation, and carry out with reference to "Pesticide Indoor Bioassay Test Guidelines for Herbicides". See 2.3.2 for the medication method, and the stem and leaf treatment method is adopted. After treatment, they were transferred to the greenhouse for routine cultivation.
[0109] 4) Data investigation and statistical analysis
[0110] 4.1), investigation method
[0111] Using the absolute number investigation method, the whole seedlings of surviving weeds were cut off along the soil surface with a blade, and the fresh weight of the weeds was weighed with an analytical balance. For dead weeds, the fresh weight is zero.
[0112] 4.2), investigation time and frequency
[0113] The investigation was conducted 20 days after the treatment, and a total of 1 investigation was conducted.
[0114] 4.3), statistical analysis of data
[0115] The theoretical fresh weight inhibition rate (E0=X+Y-X*Y/100) of the mixed combination of each treatment was calculated by the Gowing method, and then compared with the measured inhibition rate (E) to evaluate the combined effect of the two on weeds. E-E0 values greater than 10% are synergistic effects, less than -10% are antagonistic effects, and between -10% and 10% are additive effects. And according to the actual control effect and herbicide characteristics, formula balance and other factors to determine the best ratio. In the formula, X is the fresh weight inhibition rate when the amount of active ingredient A is P; Y is the fresh weight inhibition rate when the amount of active ingredient B is Q.
[0116] Soil sealing treatment:
[0117] 1) Test conditions
[0118] 1.1), test target
[0119] Broken rice chestnuts were collected from Xinghua, Jiangsu.
[0120] 1.2), culture conditions
[0121] It is carried out in a controllable solar greenhouse, with a temperature of 20 to 30°C, natural light, and a relative humidity of 57% to 72%. The soil type is loam, the organic matter content is 1.63%, pH=7.1, alkaline hydrolyzable nitrogen 84.3 mg/kg, available phosphorus 38.5 mg/kg, available potassium 82.1 mg/kg. The test soil was quantitatively filled to 3/4 of the pot, and then watered from the bottom of the pot to make the soil completely wet to saturation. The pretreated weed seeds for testing were evenly and quantitatively sown on the surface, covered with soil 0.5-2 cm according to the size of the seeds, and used 24 hours after sowing.
[0122] 1.3), instruments and equipment
[0123] GA10 type 1/10,000 electronic balance (Germany); ZDR2000 intelligent data recorder (Hangzhou Zeda Instrument Co., Ltd.); SPX type intelligent biochemical incubator (Ningbo Jiangnan Instrument Factory), pipette, etc.
[0124] 2) Experimental design
[0125] 2.1), reagents
[0126] The original medicines are all made of acetone as solvent, diluted with 0.1% emulsifier T-80 aqueous solution, and diluted with current use.
[0127] 2.2), test processing
[0128] 2.2.1), dose setting
[0129] When determining the proportion or content of each component of A and active ingredient B, it should be measured from the action characteristics and toxicity of the two agents, and the main purpose of use of the formula should also be considered. In this study, on the basis of the preliminary test, the single and mixed dosages of A and B active ingredients are shown in the table respectively. The water containing no drug, the same solvent and emulsifier was used as a blank control.
[0130] 2.2.2), test repetition
[0131] Each treatment was repeated 4 times, with 3 pots per treatment, and 30 weed seeds were sown in each pot.
[0132] 3) Test method
[0133] Place the pots for sowing and covering soil evenly on an area of 0.5m 2 On the platform, use 3WP-2000 type walking spray tower to spray the soil surface, and the spray amount is 675 kg/ha. The spray pressure is 0.3MPa. After all the liquid medicine is sprayed, close the air valve, open the spray tower door after 30 seconds, and take out the nutrition bowl. Then open the air valve, spray 50mL of water, and clean the spray pipe.
[0134] 4) Data investigation and statistical analysis
[0135] It is the same as the post-emergence stem and leaf spray treatment, so it will not be repeated.
[0136] The statistical results are shown in Table 1-15 below.
[0137] Table 1A Actual control effect and combined effect evaluation of mixed sulfometazone against Camelina officinalis (soil treatment)
[0138]
[0139] Table 2A Actual control effect and combined effect evaluation of mixed Phosphorus serrata against Camelina officinalis (soil treatment)
[0140]
[0141]
[0142] Table 3A Actual control effect and combined effect evaluation of mixed oxyfluorfen on Camelina officinalis (soil treatment)
[0143]
[0144] Table 4A Actual control effect and combined effect evaluation of mixed bispyrazone against Camelina officinalis (soil treatment)
[0145]
[0146] Table 5A Actual control effect and combined effect evaluation of mixed terbutin on broken rice camelina (soil treatment)
[0147]
[0148] Table 6A Actual control effect and combined effect evaluation of mixed Azafenazone against Camelina officinalis (soil treatment)
[0149]
[0150]
[0151] Table 7A Actual control effect and combined effect evaluation of mixed promethazine against Camelina officinalis (soil treatment)
[0152]
[0153] Table 8A Actual control effect and combined effect evaluation of compound Zhongdingling on broken rice chestnut (soil treatment)
[0154]
[0155] Table 9A Actual control effect and combined effect evaluation of mixed diflufenazone against Camelina officinalis (soil treatment)
[0156]
[0157] Table 10A Actual control effect and combined effect evaluation of mixed triflufenazone on Camelina officinalis (soil treatment)
[0158]
[0159]
[0160] Table 11A Actual control effect and combined effect evaluation of mixed mesotrione on Camelina officinalis (soil treatment)
[0161]
[0162] Table 12A Actual control effect and combined effect evaluation of mixed diflufenzopyr on Camelina officinalis (soil treatment)
[0163]
[0164] Table 13A Compounding Evaluation of Actual Control Effect and Combined Effect on Barnyardgrass (Stem and Leaf Treatment)
[0165]
[0166]
[0167] Table 14A The actual control effect and combined effect evaluation of the mixed cloflupyridate on Duckweed (stem and leaf treatment)
[0168]
[0169] Table 15A Actual control effect and combined effect evaluation of mixed cycloheptafen on Camelina officinalis (soil treatment)
[0170]
[0171] C) Daejeon Demonstration
[0172] Field weed effect tests were carried out using the herbicidal compositions prepared in Examples 1-15.
[0173] In 2017, a demonstration promotion test was carried out at the Xinghua test site in Jiangsu. The main weeds that occur in the field are: duck tongue, amaranth, quinoa, quinoa, barnyardgrass and so on.
[0174] experiment method:
[0175] Soil treatment: before weeds germinate, hand sprayer, water volume 45kg/667m 2 , and spray evenly on the soil surface.
[0176] Stem and leaf treatment: When the weeds are in the 3-4 leaf stage, use a manual sprayer with a water volume of 30 kg/667m 2 , spray evenly with stem and leaf spray.
[0177] The specific test agents and doses are shown in Table 16. The plot area is 50 square meters, and each treatment is repeated 4 times. See Table 16 for the investigation and control effects 20 days after application.
[0178]
[0179] Field demonstration effect of the compound composition described in Table 16
[0180]
[0181]
[0182] After a lot of tests and explorations, the present invention unexpectedly found that the composition is used to control weeds such as daffodil, amaranthus, quinoa, galanthus, barnyardgrass, etc., and has surprising and unexpected synergistic effects , This synergistic effect is more significant at low doses, which can reduce the dosage of the drug, reduce the pollution to the environment, and reduce the cost of agricultural use by rational compounding. It is highly effective for ALS and ACCase inhibitor-resistant weeds and has good application prospects. At the same time, it has been tested in wheat fields, corn fields, rice fields, peanuts, sugarcane, sorghum, millet, potatoes, rapeseed, soybeans, cotton, vegetables, bluegrass, tall fescue, Zoysia and other plants to show good selectivity and excellent The synergistic effect can be developed into herbicide mixtures with wide market value.
