A kind of high performance liquid phase detection method of hexaphenoxycyclotriphosphazene
A technology of hexaphenoxycyclotriphosphazene and high-performance liquid phase, which is applied in the field of analytical chemistry, can solve the problems of low separation of raw materials or impurities, cumbersome pretreatment steps, and difficult operation, etc. Appropriate peak time and good stability
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Embodiment 1 6
[0020] Embodiment 1 hexaphenoxy cyclotriphosphazene content determination test
[0021] The embodiment of the present invention provides a high-efficiency liquid phase detection method for hexaphenoxycyclotriphosphazene, comprising the following steps:
[0022] Hexaphenoxycyclotriphosphazene: produced by Shandong Taixing New Materials Co., Ltd.;
[0023] High performance liquid chromatography conditions:
[0024] Chromatographic column: WondaSil C18-WR, size 5μL, 4.6×150mm;
[0025] The mobile phase ratio is: a mixed solution of acetonitrile and methanol with a volume ratio of 50:10;
[0026] Use isocratic elution;
[0027] The detection wavelength is 210nm, the flow rate is 0.6mL / min, the column temperature is 27°C, the injection volume is 20μL, and the injection concentration of the sample solution is 0.1mg / mL.
[0028] Sample solution preparation: Accurately weigh about 10 mg of hexaphenoxycyclotriphosphazene into a 50 mL beaker, add an appropriate amount of acetonitril...
Embodiment 2 6
[0030] The precision test of embodiment 2 hexaphenoxy cyclotriphosphazene content determination
[0031] Hexaphenoxycyclotriphosphazene sample: 190824, produced by Shandong Taixing New Materials Co., Ltd.;
[0032] High performance liquid chromatography conditions:
[0033] Chromatographic column: WondaSil C18-WR, size 5μL, 4.6×150mm;
[0034] The mobile phase ratio is: a mixed solution of acetonitrile and methanol with a volume ratio of 50:10;
[0035] Use isocratic elution;
[0036] The detection wavelength is 210nm, the flow rate is 0.6mL / min, the column temperature is 27°C, the injection volume is 20μL, and the injection concentration of the sample solution is 0.1mg / mL.
[0037] Sample solution preparation: Accurately weigh 10.02 mg of hexaphenoxycyclotriphosphazene into a 50 mL beaker, add an appropriate amount of acetonitrile to dissolve, transfer to a 100 mL volumetric flask and dilute to the mark, and shake well. The concentration of this sample solution is 0.1002m...
Embodiment 3 6
[0041] The repeatability test of embodiment 3 hexaphenoxy cyclotriphosphazene content determination
[0042] Hexaphenoxycyclotriphosphazene sample: 1900808, produced by Shandong Taixing New Materials Co., Ltd.;
[0043] High performance liquid chromatography conditions:
[0044] Chromatographic column: Wondasil C18-WR, specification 5μL, 4.6×150mm;
[0045] The mobile phase ratio is: a mixed solution of acetonitrile and methanol with a volume ratio of 50:10;
[0046] Use isocratic elution;
[0047] The detection wavelength is 210nm, the flow rate is 0.6mL / min, the column temperature is 27°C, the injection volume is 20μL, and the injection concentration of the sample solution is 0.1mg / mL.
[0048] Sample solution preparation: Accurately weigh 6 parts of the same hexaphenoxycyclotriphosphazene sample (10.02mg, 10.06mg, 10.10mg, 10.03mg, 10.09mg, 10.13mg) into a 50mL beaker, add an appropriate amount of acetonitrile to dissolve, Transfer to a 100mL volumetric flask and dilute...
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