Unlock instant, AI-driven research and patent intelligence for your innovation.

A method for counting and stimulating Cryptocaryon cyst by sucrose suspension method

A technology to stimulate Cryptocaryon and Cryptocaryon cysts, applied in counting mechanisms/items, instruments, fish farming, etc., can solve the problems of stimulating Cryptocaryon cysts and counting difficulties, achieve accurate and reliable counting results, and facilitate immunization The effect of the study

Active Publication Date: 2021-10-29
SOUTH CHINA AGRI UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the problem of difficulty in counting the cysts of Cryptocaryon stimuli falling off from the fish body, and provides a method for counting the cysts of Cryptocaryon stimuli by a sucrose suspension method. Capsule counting, not only easy to operate, easy to use, but also accurate and reliable counting results

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for counting and stimulating Cryptocaryon cyst by sucrose suspension method
  • A method for counting and stimulating Cryptocaryon cyst by sucrose suspension method
  • A method for counting and stimulating Cryptocaryon cyst by sucrose suspension method

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach

[0014] 1. Optimization of sucrose concentration in Cryptocaryon cysts stimulated by counting by sucrose suspension method

[0015] 1. Stimulate Cryptocaryonia strains

[0016] The Cryptocaryon stimulator strain used in this experiment was collected from a pompano pomfret farm in Daya Bay, Huizhou City, Guangdong Province, and was identified as Cryptocaryon stimulator by clinical symptoms, morphological and molecular biology methods. Scrape the trophozoites from the body surface and gills of the diseased Pompano pomfret, clean and set aside.

[0017] 2. Stimulate Cryptocaryon subculture

[0018] Artificial subculture of stimulated Cryptocaryonia was carried out according to the method of Dan et al. (Dan et al., 2006). First, put the trophozoites collected in the previous step into a 500L culture tank, and put 5 healthy oval pomfrets at the same time to carry out running water culture with low water exchange. After 3 days, check whether there are white spots on the surface of...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention adopts the sucrose suspension method for the first time to count the stimulated cryptocystosis cysts, which is not only simple to operate and convenient to use, but also has accurate and reliable counting results, which greatly facilitates the immune research of fish stimulated cryptocystosis. By adding 37.5% sucrose solution into the encapsulation beaker, stirring well, taking out 20 parts of 1ml sucrose solution from the beaker with a pipette gun, and finally calculating the total amount of encapsulation.

Description

technical field [0001] The invention belongs to the field of fish disease prevention and control, and in particular relates to a method for counting and stimulating Cryptocaryon cyst by a sucrose suspension method. Background technique [0002] Cryptocaryon irritans (Cryptocaryon irritans) is a kind of protociliate, which mainly parasitizes on the skin, gills and fin rays of bony fishes in seawater, and a large number of infections often cause serious death of fish cultured in seawater and cause great economic losses (Colorni, 1987; Dickerson, 1995; Li et al., 2019). In recent years, due to the overloaded development of marine aquaculture in my country and the deterioration of the aquaculture environment, Cryptocaryoniasis has become a large-scale and frequent outbreak of severe infectious diseases, causing significant economic losses to marine fisheries. Due to the open nature of mariculture, it is difficult to eliminate the infection of Cryptocaryon irritant to fish, and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/13A01K61/10G06M1/12
CPCA01K61/00Y02A40/81
Inventor 李言伟但学明王久乐莫泽权何智昌
Owner SOUTH CHINA AGRI UNIV