A kind of microbe-enhanced bacterial agent and its application that can significantly promote the composting effect before domestic waste incineration
A domestic waste incineration and microbial technology, which is applied in the field of microbial enhanced bacterial agents, can solve the problems of increasing operating costs of waste incineration, unstable natural composting process, and reduced low-level calorific value of waste, so as to increase the calorific value of incineration and promote waste recovery. The effect of increasing the lower calorific value and promoting the leaching of water
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Embodiment 1
[0039] 1. Isolation, screening and identification of Lactobacillus pasteri GBW-HB1903
[0040] 1. Screening of Lactobacillus pasteri GBW-HB1903
[0041] Municipal domestic sewage was used as the sample, and after gradient dilution, the MRS medium containing calcium carbonate was used to pour culture to screen the colony with obvious "calcium dissolution circle". After multiple purifications, a single colony was obtained, named GBW-HB1903, and preserved.
[0042] Such as figure 1 As shown, the colony of the strain GBW-HB1903 on the MRS plate is round, milky white, 1-2 μm in diameter, smooth, moist and shiny, slightly raised in the middle, opaque, with neat edges and no halos. The strain GBW-HB1903 can grow and reproduce normally in the temperature range of 10-45°C, and its optimum growth temperature is 25-35°C; it can grow in the range of pH 6-9, and the optimum growth pH value is 6.5 ~7.5; it can grow normally when the dissolved oxygen content is less than 0.5mg / L or greater...
Embodiment 2
[0068] The preparation method of the microorganism-enhanced bacterial agent for significantly promoting the composting effect before domestic waste incineration described in the present invention is as follows:
[0069] 1. Activate Lactobacillus pasterii, Clostridium butyricum, Bacillus lentus and Bacillus psychrophilus in corresponding medium respectively, and then culture them in respective mediums.
[0070] Among them, Lactobacillus pasteri culture medium: soybean peptone 12g, yeast extract 6g, K 2 HPO 4 5g, triammonium citrate 2g, MgSO 4 ·7H 2 O0.5g, sodium acetate 5g, glucose 10g, Tween 801ml, CaCO 3 5g, MnSO 4 4H 2 O0.25g, distilled water 1000ml, pH 6.0, culture time 12 hours, culture temperature 28 ℃. Clostridium butyricum medium: glucose 10g, soybean peptone 10g, yeast extract 5g, sodium acetate 4g, sodium chloride 1g, MgSO 4 0.1g, K 2 HPO 4 2g, Na 2 CO 3 3g, 1000ml distilled water, pH 6.5, culture time 24 hours, culture temperature 32°C. The culture medium...
Embodiment 3
[0074] The preparation method of the microorganism-enhanced bacterial agent for significantly promoting the composting effect before domestic waste incineration described in the present invention is as follows:
[0075] 1. Activate Lactobacillus pasterii, Clostridium butyricum, Bacillus lentus and Bacillus psychrophilus in corresponding culture medium respectively, and then culture them in respective culture medium.
[0076] 2. Add 1.0 kg of talcum powder to the fermented broth of 1.0 L of Bacillus lentus and Bacillus psychrophilus with a volume ratio of 1:1 after cultivation. After mixing evenly, the temperature inside the tower is 82°C, and the temperature of the outlet air is 78°C. Under the condition of ℃, spray drying method was used to make solid powder, and 2kg of carrier was added again to prepare solid bacterial powder A with a bacterial content of 50 billion / g.
[0077]3. Add 1.0kg of sterilized calcium carbonate to the cultured 1.0L Clostridium butyricum and Lactoba...
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