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Nigella saliniva seed polysaccharide and extraction method thereof, and application of Nigella saliniva seed polysaccharide in preparation of medicine for treating type 2 diabetes mellitus

An extraction method and a technology for seeds are applied in the application field of preparing medicines for treating type 2 diabetes, achieving the effects of remarkable curative effect and no toxic and side effects

Active Publication Date: 2020-04-17
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, there is no Nigella sativa Related reports on the treatment of seed polysaccharides on type 2 diabetic mice

Method used

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  • Nigella saliniva seed polysaccharide and extraction method thereof, and application of Nigella saliniva seed polysaccharide in preparation of medicine for treating type 2 diabetes mellitus
  • Nigella saliniva seed polysaccharide and extraction method thereof, and application of Nigella saliniva seed polysaccharide in preparation of medicine for treating type 2 diabetes mellitus
  • Nigella saliniva seed polysaccharide and extraction method thereof, and application of Nigella saliniva seed polysaccharide in preparation of medicine for treating type 2 diabetes mellitus

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Experimental program
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Effect test

Embodiment 1

[0022] A sort of Nigella sativa The extraction method of seed polysaccharide is specifically: taking Nigella sativa 3.7 kg of seeds, add water until submerged Nigella sativa The seeds are decocted three times with water (the decocting water temperature is 80-85°C, the first decoction is 2 hours, the second decoction is 1 hour, and the third decoction is 0.5 hours). Combine the filtrates, concentrate under reduced pressure, add absolute ethanol to the concentrated solution until the final concentration of ethanol is 75%, let it stand in the refrigerator overnight (12 h), centrifuge, collect the precipitate, and use the Sevage method to remove protein after the precipitate is dissolved in water (choose volume ratio It is 4:1 chloroform-n-butanol mixture, and conventional techniques in this field can be used). Add absolute ethanol to the supernatant to a final concentration of 70%, let it stand in the refrigerator overnight (12 h), rinse the precipitate with acetone, petroleum ...

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Abstract

The invention discloses an extraction method for Nigella saliniva seed polysaccharide. The method comprises the following concrete steps of: decocting Nigella saliciva seeds with water, carrying out filtering, adding absolute ethyl alcohol into a filtrate until a final concentration is 70 + / -5 V%, carrying out standing, collecting a precipitate, removing protein by a Sevage method after water dissolution of the precipitate, taking a supernatant, adding absolute ethyl alcohol until a final concentration is 70 + / -5 V%, performing standing, washing a precipitate, and freeze-drying the washed precipitate to obtain the Nigella saliniva seed polysaccharide. Experiments show that the Nigella saliniva seed polysaccharide can significantly reduce the levels of FBG, glycated serum protein (GSP), triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), TNF-alpha, IL-6 and IL-1 beta of mice, and can significantly reduce the levels of FBG, glycated serum protein GSP,triglyceride TG, total cholesterol TC, low-density lipoprotein cholesterol LDL-C and increase the expression levels of insulin (INS), high-density lipoprotein cholesterol (HDL-C) and total antioxidantcapacity (T-AOC). The results of intestinal flora composition analysis show that the abundance of the f_Microbacterium_Unclassified bacterial genus and the Bacteroides genus in a model group is obviously reduced, and the Nigella saliniva seed polysaccharide remarkably increases the abundance of the f_Microbacterium_Unclassified bacterial genus and the Bacteroides genus, which indicates that the Nigella saliniva seed polysaccharide can improve the abnormal state of diabetic mice by regulating intestinal microbiota, and is an ideal medicine for treating type 2 diabetes mellitus.

Description

technical field [0001] The invention belongs to the technical field of polysaccharide medicines, and specifically relates to a Nigella sativa Seed polysaccharide, its extraction method and its application in the preparation of medicine for treating type 2 diabetes. Background technique [0002] Type 2 diabetes mellitus (T2DM) is a complex chronic metabolic disorder, mainly caused by pancreatic β-cell dysfunction caused by insulin resistance and insulin deficiency; it is characterized by disorders of sugar, lipid and protein metabolism, while insulin resistance is accompanied by Generation of low-grade chronic inflammation. T2DM can cause a variety of organ damage, resulting in a variety of complications. For example, diabetic nephropathy, diabetic foot, diabetic neuropathy and cardiovascular and cerebrovascular diseases have become major health problems worldwide. [0003] The increasing prevalence of type 2 diabetes has become a global public health concern in the 21st c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P3/10
CPCA61K31/715A61P3/10C08B37/00C08B37/0003
Inventor 康文艺马常阳王金梅刘振花董靖蒋圣军梁琼心
Owner HENAN UNIVERSITY
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