Construction and identification method of origin traceability model of sea cucumber based on lead pb stable isotope
A stable isotope and origin traceable technology, applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of nutritional quality differences and price differences, and achieve the effect of fewer detection indicators, simple methods, and high detection accuracy
Active Publication Date: 2021-08-06
QINGDAO YIHAIFENG AQUATIC PRODS
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Problems solved by technology
Due to the different growth environments of japonicus from different origins, there are great differences in nutritional quality, resulting in great differences in prices
Method used
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Embodiment 1
[0025] 1) Sample collection
[0026] From October to November 2018, 26 sea cucumber samples were collected from the main production area of sea cucumber in Qingdao, Shandong, and 14 samples were collected from the main production area of sea cucumber in Dalian, Liaoning. In March 2019, 20 samples of sea cucumber from Xiapu, Fujian were randomly collected. The total number of sea cucumber samples is 60.
[0027] 2) Freeze-drying and crushing of samples
[0028] The collected sea cucumber samples were all adult ginseng. They were packaged in sterile plastic sealed bags during transportation, stored in cold storage, and transported back to the laboratory. They were immediately rinsed with ultrapure water, dissected to remove intestinal glands and lime mouth, and their body walls were collected through After vacuum freeze-drying, it is pulverized to a particle size of 100-200 mesh.
[0029] 3) Pb stable isotope ratio determination
[0030] Sample digestion: Accurately weigh...
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The invention relates to a construction and identification method of a japonicus origin traceability model based on lead and Pb stable isotopes, which belongs to the field of aquatic product origin traceability. The ratio of Pb isotope, discriminant analysis and cross-validation were carried out to determine the establishment method of the traceability identification model of sea cucumber from different origins. The present invention simultaneously establishes identification models of sea cucumbers from three producing areas of Qingdao in Shandong, Dalian in Liaoning and Xiapu in Fujian. The invention is a method for tracing the origin of sea cucumber based on stable isotope fingerprints, which uses the Pb stable isotope fingerprint features with large mass numbers and is not prone to fractionation to establish an origin discrimination model, has few detection indicators, high detection accuracy, good reproducibility, and is simple and convenient. , strong operability, reducing the uncertainty based on commodity labels and subjective judgments, and the overall discrimination rate of sea cucumber origins is over 91%.
Description
technical field [0001] The invention belongs to the field of origin traceability of aquatic products, and in particular relates to a method for constructing and identifying a sea cucumber origin traceability model based on lead and Pb stable isotopes. Background technique [0002] Apostichopus, also known as Apostichopus japonicas (Selenka), has high nutritional value and has various biological activities such as anticoagulant and antitumor. As people pay more and more attention to the nutritional value of sea cucumber and its products, the consumption demand of sea cucumber is increasing rapidly. Sea cucumber farming has become another emerging pillar industry in the aquaculture industry after kelp, prawns, scallops, and marine fish farming. Sea cucumbers are naturally distributed in Liaoning, Shandong Peninsula and other places along the coast of the Yellow Sea and Bohai Sea. However, in recent years, with continuous breakthroughs in seedling cultivation and cultivation ...
Claims
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IPC IPC(8): G01N27/626
CPCG01N27/62
Inventor 赵艳芳康绪明宁劲松尚德荣翟毓秀丁海燕盛晓风
Owner QINGDAO YIHAIFENG AQUATIC PRODS