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Crocodile egg white hydrolysate as well as preparation method and application thereof

A crocodile and water technology is applied to crocodile egg white hydrolyzate and its preparation and application fields to achieve the effects of improving anxiety-like behavior and memory loss behavior, improving space exploration ability and object recognition ability

Active Publication Date: 2020-04-28
福建鼍龙实业有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the industrial research on egg white polypeptide technology is limited to traditional poultry such as eggs and duck eggs.

Method used

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  • Crocodile egg white hydrolysate as well as preparation method and application thereof
  • Crocodile egg white hydrolysate as well as preparation method and application thereof
  • Crocodile egg white hydrolysate as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Preparation of crocodile egg white hydrolyzate: peel crocodile egg white, crocodile eggshell, and crocodile egg yolk, mix 10 g of crocodile egg white with 50 ml of double distilled water, and crush for 10 min-20 min (tissue masher DS-1, Shanghai specimen model) plant), using 0.5-1.0mol / L NaOH to adjust the pH to 10.25±0.5, according to the mass g of alkaline protease (purchased from Sangon Bioengineering (Shanghai) Co., Ltd., Shanghai, China) and the mass of crocodile egg white after crushing The mass g ratio is 3.79%±0.1% (g / g), adding alkaline protease, the enzymolysis time is 4.63h±30min, the enzymolysis temperature is 55.59±0.5°C, and the boiling water bath inactivation time is 15±1min. Centrifuge at 12000±100g for 30±2min at 4°C. Freeze-drying to obtain crocodile egg white hydrolyzate freeze-dried powder. The degree of hydrolysis of the obtained crocodile egg white hydrolyzate was 0.852.

Embodiment 2

[0083] According to the method of Example 1.1, under the condition of pH 11.4, 5 g of crocodile egg white is mixed with 50 ml of double distilled water, and the mass g ratio of the crocodile egg white after the mass g of alkaline protease and the crushing treatment is 4% (g / g), After hydrolysis for 3 hours, the degree of hydrolysis of the obtained crocodile egg white hydrolyzate was 0.851.

Embodiment 3

[0085] According to the method of Example 1.1, under the condition of pH 9.4, 20 g of crocodile egg white is mixed with 240 ml of double distilled water, and the mass g ratio of the crocodile egg white after the mass g of alkaline protease and the crushing treatment is 3% (g / g), After hydrolysis for 3 hours, the degree of hydrolysis of the obtained crocodile egg white hydrolyzate was 0.6851.

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Abstract

The present application discloses a crocodile egg white hydrolysate. The crocodile egg white hydrolysate contains chondroitin sulfate, amino acids and small molecule peptides, wherein 5.8%-25.8% by mass of the chondroitin sulfate is contained in the crocodile egg white hydrolysate, and the amino acids and small molecule peptides include the following materials: threonine, serine, valine, glutamicacid, methionine, glycine, lysine, histidine, isoleucine, arginine, leucine, alanine, tyrosine, proline, aspartic acid and tyrosine. The crocodile egg white hydrolysate scavenges free radicals, promotes regeneration of hippocampal nerve cells, promotes expression of BDNF and CREB1, activates phosphorylation of PKA, ERK and the CREB1, and inhibits expression of GSK-3; and the hydrolysate improves expression of mRNA of antioxidant enzymes in hippocampus, inhibits acetylcholinesterase, improves anxiety-like behavior and memory decline behavior, and improves space exploration ability and object recognition ability.

Description

technical field [0001] This application belongs to the field of biological products, in particular, it relates to the use of crocodile egg white hydrolyzate and its preparation method and application. The crocodile egg white hydrolyzate can repair the hippocampus and cerebral cortex through the PKA / ERK / CREB1 / BDNF signaling pathway And to improve the memory ability of aged mice, it can be applied to the fields of health care products that can improve the memory of the elderly, anti-Alzheimer's disease drugs, etc. Background technique [0002] Siamese crocodile is a medium-sized crocodile, mainly distributed in Vietnam, Laos, Myanmar, Thailand, Cambodia, Brunei, Malaysia, Indonesia and other places. It inhabits swamps in tropical and subtropical regions, areas with slow water flow in protected areas of rivers, freshwater waters such as lakes, and brackish waters. Siamese crocodile has the advantages of low feeding cost, strong reproductive ability, quick feeding and remarkabl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L15/00A23L33/00A61K35/58A61P25/28
CPCA23L15/25A23L33/00A61K35/58A61P25/28A23V2002/00A23V2200/322
Inventor 刘笑天熊佑熊李华亮董欣陈清西
Owner 福建鼍龙实业有限责任公司
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