Method for promoting saccharification of lignocelluloses

Abstract

The invention relates to a method for promoting saccharification of lignocelluloses. The method comprises the following steps: adding a bola-type lipopeptide in a lignocellulose enzymolysis process, and the sequence of the lipopeptide is NH2-KCnK-NH2, wherein Cn represents a hydrophobic alkyl chain (n is more than or equal to 12 and less than or equal to 18), and K represents lysine. According tothe invention, the bola-type lipopeptide is used as an enzymolysis auxiliary agent, so that the enzymolysis efficiency and the concentration of fermentable sugar are improved, and the method has the characteristics of high saccharification rate, small usage amount of auxiliary agents and the like.

Description

technical field

[0001] The invention belongs to the technical field of biomass energy, and in particular relates to a method for promoting enzymatic hydrolysis and saccharification of lignocellulose. Background technique

[0002] As a rich and cheap renewable resource, cellulose is one of the most abundant biomass in nature, accounting for 50% of the total biomass in the world, and the dry matter produced by photosynthesis of green plants around the world can reach 1.55×10 per year. 11 Tons, including agricultural and forestry waste, waste paper, energy crops, etc., most of which are currently unused and discarded, of which the carbohydrate content is as high as 75%, which can be used as an important source of fermentable sugars to produce liquid fuels and other chemicals. With the shortage of petroleum resources and people's increasing attention to energy and environmental issues, using cellulose biomass as raw material and converting it into biomass energy that can replace...

Examples

Embodiment 1

[0031] Synthesis of NH by Microwave Assisted Fmoc Solid Phase Method 2 -KC 16 K-NH 2 , the specific steps are:

[0032] (1) Deprotection: Use 20% piperidine / DMF solution to remove the Fmoc protecting group 9-fluorenylmethoxycarbonyl of the amino group on the Rink amide-MBHA resin.

[0033] (2) Activation: Use an activator to activate the carboxyl group of lysine. The activator is HOBT and HBTU (ratio 1:1), DMF is the solvent, and the concentration is 0.45mol / L.

[0034] (3) Cross-linking: After the carboxyl group of lysine is activated, it undergoes an acylation reaction with the free amino group in the presence of microwave-assisted heating and an activated base to form a peptide bond. The activated base is DIEA, DMF is the solvent, the concentration is 2mol / L, and the reaction temperature is 75°C.

[0035] (4) Synthesis: Repeat steps (1)-(3) to make the dibasic acid COOH-C in the sequence 16 -COOH is connected to the resin to obtain a solid phase carrier with the targe...

Embodiment 2

[0040] With embodiment 1, difference is: get lipopeptide NH 2 -KC 16 K-NH 2 Dissolve it in a citric acid-sodium citrate buffer solution with a pH of 5, the concentration is 2g / L, and then add it in an amount of 0.10% of the total mass of the enzymatic hydrolysis system, and the saccharification rate is 84.47%.

Embodiment 3

[0042] With embodiment 1, difference is: with dibasic acid COOH-C 14 -COOH as raw material, NH synthesized by microwave-assisted Fmoc solid-phase method 2 -KC 14 K-NH 2 , The saccharification rate is 81.67%.

technical field

[0001] The invention belongs to the technical field of biomass energy, and in particular relates to a method for promoting enzymatic hydrolysis and saccharification of lignocellulose. Background technique

[0002] As a rich and cheap renewable resource, cellulose is one of the most abundant biomass in nature, accounting for 50% of the total biomass in the world, and the dry matter produced by photosynthesis of green plants around the world can reach 1.55×10 per year. 11 Tons, including agricultural and forestry waste, waste paper, energy crops, etc., most of which are currently unused and discarded, of which the carbohydrate content is as high as 75%, which can be used as an important source of fermentable sugars to produce liquid fuels and other chemicals. With the shortage of petroleum resources and people's increasing attention to energy and environmental issues, using cellulose biomass as raw material and converting it into biomass energy that can replace...

Examples

Embodiment 1

[0031] Synthesis of NH by Microwave Assisted Fmoc Solid Phase Method 2 -KC 16 K-NH 2 , the specific steps are:

[0032] (1) Deprotection: Use 20% piperidine / DMF solution to remove the Fmoc protecting group 9-fluorenylmethoxycarbonyl of the amino group on the Rink amide-MBHA resin.

[0033] (2) Activation: Use an activator to activate the carboxyl group of lysine. The activator is HOBT and HBTU (ratio 1:1), DMF is the solvent, and the concentration is 0.45mol / L.

[0034] (3) Cross-linking: After the carboxyl group of lysine is activated, it undergoes an acylation reaction with the free amino group in the presence of microwave-assisted heating and an activated base to form a peptide bond. The activated base is DIEA, DMF is the solvent, the concentration is 2mol / L, and the reaction temperature is 75°C.

[0035] (4) Synthesis: Repeat steps (1)-(3) to make the dibasic acid COOH-C in the sequence 16 -COOH is connected to the resin to obtain a solid phase carrier with the targe...

Embodiment 2

[0040] With embodiment 1, difference is: get lipopeptide NH 2 -KC 16 K-NH 2 Dissolve it in a citric acid-sodium citrate buffer solution with a pH of 5, the concentration is 2g / L, and then add it in an amount of 0.10% of the total mass of the enzymatic hydrolysis system, and the saccharification rate is 84.47%.

Embodiment 3

[0042] With embodiment 1, difference is: with dibasic acid COOH-C 14 -COOH as raw material, NH synthesized by microwave-assisted Fmoc solid-phase method 2 -KC 14 K-NH 2 , The saccharification rate is 81.67%.